LOCUS       sequence-471111-       10813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast two-hybrid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Sung YC, Li Y, Bernasconi Z, Baik S, Asuke S, Keller B, Fahima T, 
            Coaker G
  TITLE     Wheat tandem kinase RWT4 directly binds a fungal effector to 
            activate defense.
  JOURNAL   Nat Genet. 2025 Apr 14. doi: 10.1038/s41588-025-02162-w.
  PUBMED    40229601
REFERENCE   2  (bases 1 to 10813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2025 Apr 14. doi: 10.1038/s41588-025-02162-w."
COMMENT     Sequence Label: pGADT7-RWT4Ca_Reg1
FEATURES             Location/Qualifiers
     source          1..10813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             422..442
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             458..799
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     805..824
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        805..823
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      830..839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             842..868
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    875..899
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3656..3721
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(3728..3752)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4142..4329
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4197..4221)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(4446..5540)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(5541..5946)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(5988..6004)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5988..6004)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6001..6023)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6012..6028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6036..6042,6043..6060,6061..6066))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6081..6102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    6157..6190
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    6268..6301
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6370..6387)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6541..7129)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6621..6640)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7300..8091,8092..8160))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7923..7942
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8161..8265)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8333..8351
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8389..8411)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8511..8530
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      8547..9711
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        join(10485..10813,1..376)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
ORIGIN
        1 cttttctctc tcccccgttg ttgtctcacc atatccgcaa tgacaaaaaa atgatggaag
       61 acactaaagg aaaaaattaa cgacaaagac agcaccaaca gatgtcgttg ttccagagct
      121 gatgaggggt atctcgaagc acacgaaact ttttccttcc ttcattcacg cacactactc
      181 tctaatgagc aacggtatac ggccttcctt ccagttactt gaatttgaaa taaaaaaaag
      241 tttgctgtct tgctatcaag tataaataga cctgcaatta ttaatctttt gtttcctcgt
      301 cattgttctc gttccctttc ttccttgttt ctttttctgc acaatatttc aagctatacc
      361 aagcatacaa tcaactccaa gctttgcaaa gatggataaa gcggaattaa ttcccgagcc
      421 tccaaaaaag aagagaaagg tcgaattggg taccgccgcc aattttaatc aaagtgggaa
      481 tattgctgat agctcattgt ccttcacttt cactaacagt agcaacggtc cgaacctcat
      541 aacaactcaa acaaattctc aagcgctttc acaaccaatt gcctcctcta acgttcatga
      601 taacttcatg aataatgaaa tcacggctag taaaattgat gatggtaata attcaaaacc
      661 actgtcacct ggttggacgg accaaactgc gtataacgcg tttggaatca ctacagggat
      721 gtttaatacc actacaatgg atgatgtata taactatcta ttcgatgatg aagatacccc
      781 accaaaccca aaaaaagaga tctttaatac gactcactat agggcgagcg ccgccatgga
      841 gtacccatac gacgtaccag attacgctca tcaaacaagt ttgtacaaaa aagcaggctc
      901 caccatgatg ggtggctatg agttccagag ggcagaattg gacgcccttg agggtgtagt
      961 tcgtgaccca accgccgaac ccatgtcttt aacactacct cttttgcgtc acattacgaa
     1021 tgatttctcc cctgagttcg agatatctaa ggacgactcc gccgtggtat atctaggagt
     1081 gctaccatca ggatttaggg tggcagtgaa gaaatcacat tttaggtttt gtctggacga
     1141 cgaggacgcc ttcactaatg aagtttcaat tgcaatgaaa gccgcacata agaatactgt
     1201 aagagtaatc ggctattgtc atcatacaca tgagcagatt gccgagtatg agggaaagca
     1261 agtcttcgca gaacttacgg agaggttgat atgtactgag tacgtgccca atggacctct
     1321 atccggccat atcgaaggca aaatttgtgc acaaatggac ggctacgagt tccagcgtgc
     1381 tgagcttgat gcattggaga gagttgttag agatacctct gccgagccaa tgtccttgac
     1441 actgcccctt ttgcgtcata tcacaaatga tttctcagat gaatccagga tcggacgtgg
     1501 aggattcgct gttgtctatc ttggtgtttt gccatccggc ctgagaatag ctgttaagag
     1561 gttgtcaaat atagcttata tgaatgaaag tgcattccag aacgaagtat tcatcacaat
     1621 gaaagccaca cataaaaata cagtgagatt catgggatac tgctcacaga ttcaaggcaa
     1681 actaatagaa catgatggac aacatgtctt tgcacagctt gaagagaggc taatttgtgt
     1741 ggaatacgca cctaagggaa cattagacgc tcacatcgga gattatggtg aactggactg
     1801 gaaccaaaga tatcagattc tgaaaggcat atgtcagggt ttacatcatc tacatgacga
     1861 aatgcatgtc tttcatggag atatcaaacc cgccaacatt cttatcggcg ataacctagt
     1921 tcctaaaatc tatgatttcg gattgagtca aatgttcgag gaggaggaaa cggaaagaat
     1981 tgtggaaaac attgccggta cattcggcta catggcacct gagttctgta caaacaacat
     2041 ggtatccttc aaggccgaga tttacagttt aggcgttgta ataggagaac ttcttatcgg
     2101 taaaaaagga tggtttgacg aggacgtgcg gaagcttttt gttcagcagt tgaaaggcct
     2161 tcgcaaaact ttggtgaagg aaggcgcgtt ttccagctgg gagaataaat accatcaagt
     2221 gcgcacgtgt atggagattg gtcaggattg catcgatcca aatcctcata aacggcctac
     2281 gcttctcgaa attattcaac gccttaacga agccgaagac atgaattata gtgcggcatc
     2341 actctggcag gttcagtctg gtgatgaaga atcagatctc tccgatactg aagcgttgga
     2401 gacagagacc acgagcgagt ttcttccgtc cgatgaagaa ccggcgtccg tcggtaagac
     2461 cggcgagacc tctacacagg aaccggacaa acccgatctt atatctaaac ttcccgcttc
     2521 ggttgacctt tctgatctca aggtgcttga aaagataacc gatgatttta gccatgagag
     2581 aatagtcggg aaagacggga cttttaaagg atgccataag gctttcgttt ataaaggtga
     2641 cattcctctg agggagatga ttgcagttaa acggctcatt ggtgttgaga tacccttcga
     2701 gaagttcaag agagaggctg aacaattcat cagtttggat cataagaaca tagttaaggt
     2761 tgcaagttat tgccacgacc agagccgggg acatagactc gtccagttca aggggaagcc
     2821 tctgccccaa ctttttaatg ggcctgagca actgttgtgt tacgagtaca tgcacaatgg
     2881 ttctcttaga gattacttga tgggccaagg ctcccgggtc atagattggc agatgcggta
     2941 caagctgata aaagggacct gcgcaggctt gcattatctt cataagggtc gcgcaggatg
     3001 tccgatcgtt caccttaatc tgagtcccag caacgtgctt ttggatcata attacattcc
     3061 gcggataact ggcttcgact tttccaagct tataggagaa aaaaacacta aatctgtggt
     3121 cttgaagctc aatggaccca tcgcttatct gccccccgac ttcttccaca gcaaaggtac
     3181 agatctcaag tacctggcga cagttgatat atactctttg ggcctgatga tactcgagat
     3241 tgcaacacag caggaaatca agggcataca tggtgttctg ataaagtcaa tcgaggaaaa
     3301 ctggcgggaa gagtcccaaa tcacacgcct ctatacctct cttggggctg atgagctgag
     3361 gcaggtgaag atgtgtattg atataggact tgactgtgtc aagagtaacc cagagaagcg
     3421 ccccactgca ggagcaatta tgttgtggct tgataaagaa tctaagccag tccccgtgtc
     3481 acgggccgga gcgggcgttt tgccacggcc cccagttccg accaacatca accatgcggg
     3541 gcgcatccag ggtatcccaa tcgagaaatc acaagtcctt attccttcat tcgcttgtaa
     3601 tgaatatagc tctgtcaaaa ttcaaaattt gcacttgctg ctctttatgg gtagcgacta
     3661 caaggaccac gacggtgact acaaggacca cgacatcgac tacaaggacg acgacgacaa
     3721 gatctagacc cagctttctt gtacaaagtg gttcgatcga gctgcagatg aatcgtagat
     3781 actgaaaaac cccgcaagtt cacttcaact gtgcatcgtg caccatctca atttctttca
     3841 tttatacatc gttttgcctt cttttatgta actatactcc tctaagtttc aatcttggcc
     3901 atgtaacctc tgatctatag aattttttaa atgactagaa ttaatgccca tctttttttt
     3961 ggacctaaat tcttcatgaa aatatattac gagggcttat tcagaagctt tggacttctt
     4021 cgccagaggt ttggtcaagt ctccaatcaa ggttgtcggc ttgtctacct tgccagaaat
     4081 ttacgaaaag atggaaaagg gtcaaatcgt tggtagatac gttgttgaca cttctaaata
     4141 agcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt
     4201 atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc
     4261 tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctctta ttgaccacac
     4321 ctctaccggc cggtcgaaat tcccctaccc tatgaacata ttccattttg taatttcgtg
     4381 tcgtttctat tatgaatttc atttataaag tttatgtaca aatatcataa aaaaagagaa
     4441 tctttttaag caaggatttt cttaacttct tcggcgacag catcaccgac ttcggtggta
     4501 ctgttggaac cacctaaatc accagttctg atacctgcat ccaaaacctt tttaactgca
     4561 tcttcaatgg ccttaccttc ttcaggcaag ttcaatgaca atttcaacat cattgcagca
     4621 gacaagatag tggcgatagg gttgacctta ttctttggca aatctggagc agaaccgtgg
     4681 catggttcgt acaaaccaaa tgcggtgttc ttgtctggca aagaggccaa ggacgcagat
     4741 ggcaacaaac ccaaggaacc tgggataacg gaggcttcat cggagatgat atcaccaaac
     4801 atgttgctgg tgattataat accatttagg tgggttgggt tcttaactag gatcatggcg
     4861 gcagaatcaa tcaattgatg ttgaaccttc aatgtaggaa attcgttctt gatggtttcc
     4921 tccacagttt ttctccataa tcttgaagag gccaaaacat tagctttatc caaggaccaa
     4981 ataggcaatg gtggctcatg ttgtagggcc atgaaagcgg ccattcttgt gattctttgc
     5041 acttctggaa cggtgtattg ttcactatcc caagcgacac catcaccatc gtcttccttt
     5101 ctcttaccaa agtaaatacc tcccactaat tctctgacaa caacgaagtc agtaccttta
     5161 gcaaattgtg gcttgattgg agataagtct aaaagagagt cggatgcaaa gttacatggt
     5221 cttaagttgg cgtacaattg aagttcttta cggattttta gtaaaccttg ttcaggtcta
     5281 acactacctg taccccattt aggaccaccc acagcaccta acaaaacggc atcagccttc
     5341 ttggaggctt ccagcgcctc atctggaagt gggacacctg tagcttcgat agcagcacca
     5401 ccaattaaat gattttcgaa atcgaacttg acattggaac gaacatcaga aatagcttta
     5461 agaaccttaa tggcttcggc tgtgatttct tgaccaacgt ggtcacctgg caaaacgacg
     5521 atcttcttag gggcagacat tagaatggta tatccttgaa atatatatat atattgctga
     5581 aatgtaaaag gtaagaaaag ttagaaagta agacgattgc taaccaccta ttggaaaaaa
     5641 caataggtcc ttaaataata ttgtcaactt caagtattgt gatgcaagca tttagtcatg
     5701 aacgcttctc tattctatat gaaaagccgg ttccggcgct ctcacctttc ctttttctcc
     5761 caatttttca gttgaaaaag gtatatgcgt caggcgacct ctgaaattaa caaaaaattt
     5821 ccagtcatcg aatttgattc tgtgcgatag cgcccctgtg tgttctcgtt atgttgagga
     5881 aaaaaataat ggttgctaag agattcgaac tcttgcatct tacgatacct gagtattccc
     5941 acagttgggg gatctcgact ctagctagag gatcaattcg taatcatgtc atagctgttt
     6001 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6061 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6121 cccgctttcc agtcgggaaa cctgtcgtgc cagctgataa cttcgtataa tgtatgctat
     6181 acgaagttat taggtctgaa gaggagttta cgtccagcca agctagcttg gctgcaggtc
     6241 gagcggccgc gatccggaac ccttaatata acttcgtata atgtatgcta tacgaagtta
     6301 tcagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     6361 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6421 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6481 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6541 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6601 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6661 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6721 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6781 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6841 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6901 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6961 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     7021 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7081 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7141 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7201 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7261 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7321 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7381 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7441 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7501 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7561 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7621 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7681 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7741 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7801 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7861 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7921 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7981 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     8041 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     8101 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     8161 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8221 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8281 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     8341 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     8401 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     8461 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     8521 gcagattgta ctgagagtgc accataacgc atttaagcat aaacacgcac tatgccgttc
     8581 ttctcatgta tatatatata caggcaacac gcagatatag gtgcgacgtg aacagtgagc
     8641 tgtatgtgcg cagctcgcgt tgcattttcg gaagcgctcg ttttcggaaa cgctttgaag
     8701 ttcctattcc gaagttccta ttctctagct agaaagtata ggaacttcag agcgcttttg
     8761 aaaaccaaaa gcgctctgaa gacgcacttt caaaaaacca aaaacgcacc ggactgtaac
     8821 gagctactaa aatattgcga ataccgcttc cacaaacatt gctcaaaagt atctctttgc
     8881 tatatatctc tgtgctatat ccctatataa cctacccatc cacctttcgc tccttgaact
     8941 tgcatctaaa ctcgacctct acatttttta tgtttatctc tagtattact ctttagacaa
     9001 aaaaattgta gtaagaacta ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat
     9061 ttcctatacg tagtatatag agacaaaata gaagaaaccg ttcataattt tctgaccaat
     9121 gaagaatcat caacgctatc actttctgtt cacaaagtat gcgcaatcca catcggtata
     9181 gaatataatc ggggatgcct ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca
     9241 gtaaacgcgg gaagtggagt caggcttttt ttatggaaga gaaaatagac accaaagtag
     9301 ccttcttcta accttaacgg acctacagtg caaaaagtta tcaagagact gcattataga
     9361 gcgcacaaag gagaaaaaaa gtaatctaag atgctttgtt agaaaaatag cgctctcggg
     9421 atgcattttt gtagaacaaa aaagaagtat agattctttg ttggtaaaat agcgctctcg
     9481 cgttgcattt ctgttctgta aaaatgcagc tcagattctt tgtttgaaaa attagcgctc
     9541 tcgcgttgca tttttgtttt acaaaaatga agcacagatt cttcgttggt aaaatagcgc
     9601 tttcgcgttg catttctgtt ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag
     9661 cgctctcgcg ttgcattttt gttctacaaa atgaagcaca gatgcttcgt tgcttgcatg
     9721 cctgcaggtc gagatccggg atcgaagaaa tgatggtaaa tgaaatagga aatcaaggag
     9781 catgaaggca aaagacaaat ataagggtcg aacgaaaaat aaagtgaaaa gtgttgatat
     9841 gatgtatttg gctttgcggc gccgaaaaaa cgagtttacg caattgcaca atcatgctga
     9901 ctctgtggcg gacccgcgct cttgccggcc cggcgataac gctgggcgtg aggctgtgcc
     9961 cggcggagtt ttttgcgcct gcattttcca aggtttaccc tgcgctaagg ggcgagattg
    10021 gagaagcaat aagaatgccg gttggggttg cgatgatgac gaccacgaca actggtgtca
    10081 ttatttaagt tgccgaaaga acctgagtgc atttgcaaca tgagtatact agaagaatga
    10141 gccaagactt gcgagacgcg agtttgccgg tggtgcgaac aatagagcga ccatgacctt
    10201 gaaggtgaga cgcgcataac cgctagagta ctttgaagag gaaacagcaa tagggttgct
    10261 accagtataa atagacaggt acatacaaca ctggaaatgg ttgtctgttt gagtacgctt
    10321 tcaattcatt tgggtgtgca ctttattatg ttacaatatg gaagggaact ttacacttct
    10381 cctatgcaca tatattaatt aaagtccaat gctagtagag aaggggggta acacccctcc
    10441 gcgctctttt ccgatttttt tctaaaccgt ggaatatttc ggatatcctt ttgttgtttc
    10501 cgggtgtaca atatggactt cctcttttct ggcaaccaaa cccatacatc gggattccta
    10561 taataccttc gttggtctcc ctaacatgta ggtggcggag gggagatata caatagaaca
    10621 gataccagac aagacataat gggctaaaca agactacacc aattacactg cctcattgat
    10681 ggtggtacat aacgaactaa tactgtagcc ctagacttga tagccatcat catatcgaag
    10741 tttcactacc ctttttccat ttgccatcta ttgaagtaat aataggcgca tgcaacttct
    10801 tttctttttt ttt
//
