LOCUS       sequence-471125-       15688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15688)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 15688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0705
FEATURES             Location/Qualifiers
     source          1..15688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    11545..12133
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11598..11618)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12016..12027)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12205..12438
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12516..12637
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12553..12572)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12607..12626
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12677..12812
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12832..12851)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12833..12851)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12861..12878)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12861..12877)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12870..12892)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13019..13474
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13106..13125)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13316..13337
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13500..13604
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13605..13673,13674..14465)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13823..13842)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14636..15224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15125..15144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15378..15395
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    15512..15533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15548..15553,15554..15571,15572..15578)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15586..15602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15591..15613
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15610..15626
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15610..15626
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     15645..15665
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        15647..15665
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtca agttcaggtt atcctcccaa
     4141 ccaaggagca ttcagcacag aacaaagtcg ttatcctcct cactctgtcc agtatacatt
     4201 tcccaacacc cgccaccagc aggagttcgc agtccctgat tatcgttcct ctcatcttga
     4261 agtgagtcag gcatcacagc ttttgcagca acagcagcag caacagcttc gaaggcgacc
     4321 ttccttgctt tcagaatttc acccaggttc tgacaggcct caagaaagga gaactagtta
     4381 tgaaccgttt catccaggcc catccccagt ggatcatgat tcactggaat cgaagcgacc
     4441 acgtctggaa caggtttctg attctcattt tcagcgtgtc agtgctgcgg ttttgccttt
     4501 agtgcacccg ctgccagaag ggctgagggc ttctgcagat gctaagaagg atccagcatt
     4561 cggaggcaaa catgaagctc catcctctcc aatttcgggg caaccatgtg gagatgatca
     4621 aaatgcttca ccttcaaaac tctcaaagga agagttaata cagagtatgg atcgtgtaga
     4681 tcgagaaatt gcaaaagtag aacagcagat ccttaaactg aaaaagaaac aacaacagct
     4741 tgaagaagag gcagctaaac ctcctgagcc tgagaagccc gtgtcccctc ctcctgtgga
     4801 gcagaaacac cgcagtattg tccaaattat ttatgatgag aatcggaaaa aagcagaaga
     4861 agctcataaa atttttgaag gtcttggccc aaaagttgaa ctgccactgt ataaccagcc
     4921 atcagatacc aaggtgtacc atgagaacat caagacaaac caggtgatga ggaaaaaact
     4981 cattttattt tttaaaagaa gaaatcatgc aagaaaacaa agggaacaaa aaatctgcca
     5041 gcgttatgat cagctcatgg aggcatggga gaaaaaagtg gacagaatag aaaataatcc
     5101 tcggaggaaa gctaaagaaa gcaaaacaag ggaatactat gaaaagcagt ttccagaaat
     5161 tcgaaaacaa agagaacagc aagaaagatt tcagcgagtt gggcagaggg gagctggtct
     5221 ttcagccacc attgctagga gtgagcatga gatttctgaa attattgatg ggctctctga
     5281 gcaggagaat aatgagaaac aaatgcggca gctctctgtg attccaccta tgatgtttga
     5341 tgcagaacaa agacgagtca agttcattaa catgaatggg cttatggagg accctatgaa
     5401 agtgtataaa gataggcagt ttatgaatgt ttggactgac catgaaaagg agatctttaa
     5461 ggacaagttt atccagcatc caaaaaactt tggactaatt gcatcatact tggagaggaa
     5521 gagtgttcct gattgtgttt tgtattacta tttaaccaag aaaaatgaga attataaagc
     5581 cctcgtcaga aggaattatg ggaaacgcag aggcagaaac cagcaaattg ctcgaccctc
     5641 gcaagaagaa aaagtagaag aaaaagaaga ggataaagca gaaaaaacag aaaaaaaaga
     5701 agaagaaaag aaagatgaag aggaaaaaga tgaaaaagaa gactccaaag aaaataccaa
     5761 ggaaaaggac aagatagatg gtacagcaga agaaactgag gaaagagagc aagccacacc
     5821 ccgggggcga aagactgcca acagtcaggg ccgccgtaag ggccggatca ccaggtccat
     5881 gacaaacgaa gctgcagctg ccagtgctgc agccgcagcg gctactgaag agcccccacc
     5941 acctctgcca ccgccaccag aacccatttc tacagagcct gtggagacct ctcgatggac
     6001 agaagaagaa atggaagttg ctaaaaaagg tctagtagaa catggtcgta actgggcagc
     6061 aattgctaaa atggtgggaa cgaaaagtga agctcaatgt aaaaacttct attttaacta
     6121 taaaaggcga cacaatcttg acaacctctt acagcagcat aaacagaaaa cttcacgaaa
     6181 acctcgtgaa gagcgagatg tgtctcaatg tgaaagtgtc gcttccactg tttctgctca
     6241 ggaggatgaa gatattgaag cctccaatga agaagaaaat ccagaagaca gcgaagttga
     6301 agctgtcaag cccagcgagg acagtcctga aaatgctact tctcgaggaa acacagaacc
     6361 tgcggttgag cttgagccca ccacggaaac tgcacccagt acatctccct ccttagcagt
     6421 tccaagtaca aaaccagctg aagatgaaag tgtggagacc caggtgaatg acagcatcag
     6481 tgctgagaca gcagagcaga tggatgtaga tcagcaggag cacagtgctg aagagggttc
     6541 tgtttgtgat cccccacccg ctaccaaagc tgactctgtg gacgttgaag tgagggtgcc
     6601 agaaaaccat gcatctaaag ttgaaggtga taataccaaa gaaagagact tggatagagc
     6661 cagtgagaag gtggaaccta gagatgaaga tttggtggta gctcagcaaa taaatgccca
     6721 aaggcccgag ccccagtcag acaatgattc cagtgccacg tgcagcgctg atgaggatgt
     6781 ggatggagag ccagagaggc agagaatgtt tcctatggac tcaaagcctt cactgttaaa
     6841 ccccactgga tctatactcg tctcatctcc gttaaaacca aatccactgg atctgccaca
     6901 gcttcagcat cgagctgctg ttatcccacc aatggtatcc tgcaccccat gtaacatacc
     6961 aattggaacc ccagtgagcg gctatgctct ctaccagcga cacattaaag caatgcatga
     7021 gtcagcactc ctggaggagc agcggcagag acaagaacag atagatttgg aatgtagaag
     7081 ttctacaagt ccatgtggca catccaagag tccaaacaga gagtgggaag tccttcagcc
     7141 tgctccacat caagtgataa ctaatctccc tgaaggcgtt cggcttccga caactcgacc
     7201 aaccaggcca ccgccccctc tcatcccgtc atccaaaacc acagtggctt cagaaaaacc
     7261 atcttttata atgggaggct ccatctcaca gggaacacca ggcacttatt tgacttctca
     7321 taatcaggct tcctacactc aagaaacacc caagccgtca gtgggatcta tctctcttgg
     7381 actgccacgg caacaggaat ctgccaaatc agctactttg ccctacatca agcaggaaga
     7441 attttctccc cgaagccaaa actcacaacc tgagggtctg ttggtcaggg cccaacatga
     7501 aggtgtagtc agaggtaccg caggagccat acaagaagga agtataactc ggggaactcc
     7561 aaccagcaaa atttcagtgg agagcattcc atccctacgg ggctctatca ctcagggcac
     7621 cccggctctg ccccagactg gcataccaac agaggctttg gtgaaggggt ccatttcgag
     7681 aatgcccatt gaagacagca gtcctgagaa aggcagagag gaagctgcat ccaaaggcca
     7741 tgttatttat gaaggcaaaa gtggacatat cttgtcatat gataatatta agaatgcccg
     7801 agaagggact aggagtccaa gaacagctca tgaaatcagt ttaaagagaa gctatgaatc
     7861 agtggaagga aatataaagc aagggatgtc aatgagggag tctcctgtat cagcaccgtt
     7921 agaggggctg atatgccgag cattacccag ggggagtcct cattctgacc tcaaagaaag
     7981 gactgtattg tctggctcca taatgcaggg gacaccaaga gcaacaactg aaagctttga
     8041 agatggcctt aaatatccca aacaaattaa aagggaaagt cctcccatac gagcatttga
     8101 aggtgccatt accaaaggaa aaccatatga tggcatcacc accatcaaag aaatggggcg
     8161 ttccattcat gagattccaa ggcaagatat tttaactcag gaaagtcgga aaactccaga
     8221 agtggtccag agcacacggc cgataattga gggttccatt tcccagggca caccaataaa
     8281 gtttgacaac aactcaggtc aatctgccat caaacacaat gtcaaatcct taatcacggg
     8341 gcctagcaaa ctatcccgtg gaatgcctcc gctggaaatt gtgccagaga acataaaagt
     8401 ggtagaacgg ggaaaatatg aggatgtgaa agcaggcgag accgtgcgtt cccggcacac
     8461 gtcagtggta agctctggcc cctccgttct taggtccaca ctgcatgaag ctcccaaagc
     8521 acaactgagc cctgggattt atgatgacac cagtgcacgg aggacccctg tgagttatca
     8581 aaacaccatg tccagaggct cacccatgat gaacagaact tctgatgtta caatttcttc
     8641 taacaagtct accaatcatg aaaggaaatc gacactgacc cctacccaga gggaaagtat
     8701 cccagcgaag tctccagtgc ctggggtgga ccctgtcgtg agccacagtc cgtttgatcc
     8761 ccatcacaga ggcagcactg caggcgaggt ttatcggagc cacctgccca cgcacttgga
     8821 tccagccatg ccttttcaca gggctttgga tcctgcagcg gctgcttacc tgtttcagag
     8881 acagctttca ccaactccag gttacccaag tcagtatcag ctttacgcaa tggagaacac
     8941 aagacagaca atcttaaatg attacattac ctcacaacag atgcaagtga acttgcgtcc
     9001 agatgtggcc agaggactct ccccaagaga gcagccactg ggtctcccat acccagcaac
     9061 gagaggaatc attgacctga ccaatatgcc tccaacaatt ttagtgcctc atccaggggg
     9121 aacaagcact cctcccatgg acagaatcac ttatattcct ggtacacaga ttactttccc
     9181 tcccaggccg tacaactctg cttccatgtc tccaggacac ccaacacacc ttgcagctgc
     9241 tgcaagtgct gagagggaac gggaacggga gcgggagaag gagcgggagc gggaacggat
     9301 tgctgcagct tcctccgacc tctacctgcg gccaggctca gaacagcctg gccgacctgg
     9361 cagtcatgga tatgttcgct ccccttcccc ttcagtaaga actcaggaga ccatgttgca
     9421 acagagaccc agtgttttcc aaggaaccaa tggaaccagt gtaatcacac ctttggatcc
     9481 aactgctcag ctacgaatca tgccactgcc tgctgggggc ccttcaataa gccaaggcct
     9541 gccagcctcc cgttacaaca ctgctgcgga tgccctggct gctcttgtgg atgctgcagc
     9601 ttctgcaccc cagatggatg tgtccaaaac aaaagagagt aagcatgaag ctgccaggtt
     9661 agaagaaaat ttgagaagca ggtcagcagc agttagtgaa cagcagcagc tagagcagaa
     9721 aaccctggag gtggagaaga gatctgttca gtgtttatac acttcttcag cctttccaag
     9781 tggcaagccc cagcctcatt cttcagtagt ttattctgag gctgggaaag ataaagggcc
     9841 tcctccaaaa tccagatatg aggaagagct aaggaccaga gggaagacta ccattactgc
     9901 agctaacttc atagacgtga tcatcacccg gcaaattgcc tcggacaagg atgcgaggga
     9961 acgtggctct caaagttcag actcttctag tagcttatct tctcacaggt atgaaacacc
    10021 tagcgatgct attgaggtga taagtcctgc cagctcacct gcgccacccc aggagaaact
    10081 gcagacctat cagccagagg ttgttaaggc aaatcaagcg gaaaatgatc ctaccagaca
    10141 atatgaagga ccattacatc actatcgacc acagcaggaa tcaccatctc cccaacaaca
    10201 gctgccccct tcttcacagg cagagggaat ggggcaagtg cccaggaccc atcggctgat
    10261 cacacttgct gatcacatct gtcaaattat cacacaagat tttgctagaa atcaagtttc
    10321 ctcgcagact ccccagcagc ctcctacttc tacattccag aactcacctt ctgctttggt
    10381 atctacacct gtgaggacta aaacatcaaa ccgttacagc ccagaatccc aggctcagtc
    10441 tgtccatcat caaagaccag gttcaagggt ctctccagaa aatcttgtgg acaaatccag
    10501 gggaagtagg cctggaaaat ccccagagag gagtcacgtc tcttcggagc cctacgagcc
    10561 catctcccca ccccaggttc cggttgtgca tgagaaacag gacagcttgc tgctcttgtc
    10621 tcagaggggc gcagagcctg cagagcagag gaatgatgcc cgctcaccag ggagtataag
    10681 ctacttgcct tcattcttca ccaagcttga aaatacatca cccatggtta aatcaaagaa
    10741 gcaggagatt tttcgtaagt tgaactcctc tggtggaggt gactctgata tggcagctgc
    10801 tcagccagga actgagatct ttaatctgcc agcagttact acgtcaggct cagttagctc
    10861 tagaggccat tcttttgctg atcctgccag taatcttggg ctggaagaca ttatcaggaa
    10921 ggctctcatg ggaagctttg atgacaaagt tgaggatcat ggagttgtca tgtcccagcc
    10981 tatgggagta gtgcctggta ctgccaacac ctcagttgtg accagtggtg agacacgaag
    11041 agaggaaggg gacccatcac ctcattcagg aggagtttgc aaaccaaagc tgatcagcaa
    11101 gtcaaacagc aggaaatcta agtctcctat acctgggcaa ggctacttag gaacggaacg
    11161 gccctcttca gtctcctctg tacattcaga aggggattac cataggcaga cgccagggtg
    11221 ggcctgggaa gacaggccct cttcaacagg ctcaactcag tttccttata accctctgac
    11281 tatgcggatg ctcagcagta ctccaccaac accgattgca tgtgctccct ctgcggtgaa
    11341 ccaagcagct cctcaccaac agaacaggat ctgggagcga gagcctgccc cactgctctc
    11401 agcacagtac gagaccctgt cggatagtga tgactagtaa tgaactagtc cgtaacttga
    11461 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgatgcac ggtggccaat
    11521 aacggagaca cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
    11581 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
    11641 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
    11701 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
    11761 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
    11821 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
    11881 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
    11941 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
    12001 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
    12061 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
    12121 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
    12181 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
    12241 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
    12301 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
    12361 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
    12421 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
    12481 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
    12541 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    12601 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
    12661 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
    12721 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
    12781 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
    12841 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
    12901 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
    12961 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
    13021 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
    13081 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
    13141 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
    13201 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
    13261 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
    13321 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
    13381 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
    13441 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
    13501 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    13561 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    13621 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    13681 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    13741 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    13801 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    13861 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    13921 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    13981 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    14041 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    14101 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    14161 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    14221 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    14281 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    14341 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    14401 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    14461 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    14521 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    14581 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    14641 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    14701 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    14761 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    14821 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    14881 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    14941 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    15001 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    15061 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    15121 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    15181 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    15241 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    15301 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    15361 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    15421 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    15481 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    15541 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    15601 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
    15661 aaagggaaca aaagctggag ctgcaagc
//
