LOCUS       sequence-471137-       14086 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14086)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 14086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0065
FEATURES             Location/Qualifiers
     source          1..14086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    9943..10531
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9996..10016)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10414..10425)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10603..10836
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10914..11035
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10951..10970)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11005..11024
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11075..11210
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11230..11249)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11231..11249)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11259..11276)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11259..11275)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11268..11290)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11417..11872
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11504..11523)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11714..11735
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11898..12002
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12003..12071,12072..12863)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12221..12240)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13034..13622
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13523..13542
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13776..13793
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13910..13931
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13946..13951,13952..13969,13970..13976)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13984..14000
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13989..14011
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14008..14024
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14008..14024
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14043..14063
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14045..14063
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgatt gaagacaagg ggcctcgtgt
     4141 tgctgactac tttgttgtag caggattaac tgatgtttca aagcctctag aagaagaaat
     4201 tcacttcaat gatgcttgtc ataaagtagc taaaccaaaa gaacctatta cagatgtttc
     4261 agttattatc aaatctcttg gggaggaagt cccacaggat tatatctgta ttgatgttac
     4321 cccaactgga ttgtcagctg atcttaataa tggaagtctt gtggggccac agatttacct
     4381 ttgctataga agaggaagag ataagcctcc acttacagat ctgggggttt tatatgactg
     4441 gaaagaaaga ttgaaacagg gttgtgaaat tattcagagt actccctatg ggcgccccgc
     4501 aaatattagt gggagtacct catcacaaag aatttatatc acttaccgaa gagcctctga
     4561 aaacatgact cagaatacgt tggctgtcac tgacatatgt attattatac ccagtaaagg
     4621 agaaagccca ccacacacgt tctgcaaagt cgacaagaat ctcaataaca gtatgtgggg
     4681 atcagcagta tacttgtgtt ataaaaaatc ggtggcaaag acgaacactg tatcttacaa
     4741 agctggccta atttgtagat atcctcaaga agattatgag tcattctcac taccggaatc
     4801 tgttcctcta ttttgtttac caatgggtgc aaccattgaa tgttggccat caaatagcaa
     4861 ataccctctg cctgtatttt ctacttttgt tttaactgga gcctcagctg aaaaggttta
     4921 tggtgctgct attcagtttt atgaaccata ctctgaggag aatctcacag aaaagcagag
     4981 acttcttttg ggtttaacat cagcagatgg gaagtctgat agttccaaaa caattcatac
     5041 taacaaatgc atctgtcttc tttctcactg gccttttttt gatgcattca ggaagtttct
     5101 gacttttctg tatcgttatt ccatctctgg gcctcatgtc cttccaattg agaagcatat
     5161 ttctcatttt atgcataaag ttccttttcc atctcctcag agaccacgga ttttagttca
     5221 gttatcacca catgataatc tgattctcag tcagcctgtg tcttcacctc ttccactaag
     5281 tggtggcaag ttttcaacac tactgcagaa tttaggccct gaaaatgctg tgacactact
     5341 ggtgtttgca gtaacagaac ataaaattct tatccattcc ctacggccat ccgtgcttac
     5401 tagtgtgaca gaagcattag tgtctatgat tttccctttc cactggccat gcccgtatgt
     5461 tcctctctgc ccactggctt tagcagatgt cttgagtgca ccatgtccat tcatagtagg
     5521 gattgattca agatactttg atctctatga tcctccacca gatgtcagtt gtgttgatgt
     5581 agataccaac actatttctc aaattggtga caagaaaaat gtagcctgga agatacttcc
     5641 aaagaagcca tgtaaaaatc tgatgaacac actgaataat ttgcatcagc aattggcaaa
     5701 attgcagcag agaccgagag atgatggact catggattta gcaataaatg actatgattt
     5761 taattctgga aagaggttgc acatgataga tttggaaatc caagaggcat ttttgttttt
     5821 catggcctct attttaaaag gttacagatc atacttaagg ccaataacac aagccccatc
     5881 tgagacagcc acagatgcag cctctctctt tgccctacaa gctttcttaa gaagccggga
     5941 ccggtcacat caaaaattct ataacatgat gaccaaaaca caaatgttta ttcgcttcat
     6001 tgaagaatgt tcttttgtta gtgacaaaga tgcaagcctg gcattttttg atgattgtgt
     6061 agataaagtg gatatggaca agagcggtga agtacgactt atagaactgg atgaatcttt
     6121 caaaagtgaa cacactgttt ttgtaacacc acctgagatc ccccacctac ccaatggtga
     6181 agaaccccct ttacagtaca gctataatgg atttccagtt cttaggaaca atttatttga
     6241 aagacctgaa ggatttctac aagcaaagaa gaacaaattg ccttcaaaat caagtagtcc
     6301 taatagccct ttgcccatgt tcagaagaac aaaacaggaa attaaatcag cccataaaat
     6361 tgcaaagagg tactcttcca tccctcagat gtggtctagg tgtctactgc gccactgtta
     6421 tggactgtgg tttatttgtc tcccagctta tgtgaaagtc tgtcattcaa aagtcagggc
     6481 tctgaaaaca gcatatgatg tgcttaaaaa aatgcagtca aagaagatgg atccacctga
     6541 tgaggtatgc taccgcattc ttatgcaact ctgtggacaa tatgatcagc cagtgcttgc
     6601 agttcgagtg ctttttgaaa tgcagaaagc tggtattgac cccaatgcca ttacttatgg
     6661 ttattataat aaggctgttt tggaaagtac ctggccttca agaagtcgta gtggctattt
     6721 tctttggaca aaagtaagaa atgttgtttt aggagtaaca cagttcaaaa gagctttaaa
     6781 gaagcatgca cacttatcac aaacaactct ctcagatggc agtgacctgg atgctgttag
     6841 tcatggcagc atggatagtg gtcatgggac acacactgtg gagcaggcac cttttaatac
     6901 gggtttaatc aaagtatatg ctactgatga tagatctagt acaggtggcc agtctgatct
     6961 tggatataat tcattatcta aagatgaagt tagaagaggg gatacatcta ctgaagacat
     7021 tcaagaagaa aaagataaaa aagggagtga ttgtagttcc ttgtcagaga gtgagagtac
     7081 aaaaggaagt gctgattgcc ttcctaagct cagttatcaa aattcttcca gtatcgttcg
     7141 cctcactggt acaagtaaca acagtgctgg taaaatatca ggagaaagca tggaaagcac
     7201 acctgagctg ctcttaatat catctcttga agatacaaat gaaacacgaa acattcaaag
     7261 tagatgcttc aggaaaagac ataaaagtga caatgaaact aatttgcagc agcaagtggt
     7321 ctggggaaat agaaaccgta atcttagtgg aggggtactg atgggattta tgctcaatag
     7381 aattaaccag gaagcaaccc ctggagatat agttgaaaaa ttgggagctg atgcaaaaat
     7441 tctttcaaat gttatctcaa aaagcacgag accaaatact cttgatattg ggaagccacc
     7501 tttaagatca aaaagagaca gtctagaaaa ggaatctagt gatgatgata caccttttga
     7561 tggttctaac tatttggcag ataaagtgga ttctcctgtt atttttgact tagaagactt
     7621 agacagtgaa acagatgtat caaaagcagg atgtgttgct acacaaaatc ccaagaggat
     7681 tcaacgtatg aacagcagct tttcagtaaa accttttgaa aaaactgatg tcgcaacagg
     7741 atttgatccc ctctctcttt tggttgctga gactgaacag cagcaaaaag aagaagagga
     7801 ggaggatgaa gatgatagca aaagcatttc aacaccatct gctaggcgtg atttagctga
     7861 agaaattgtg atgtatatga ataacatgag cagtcctttg acaagtcgta caccaagcat
     7921 tgatttacaa cgggcatgtg atgataaatt aaataagaaa agtccaccat tggtcaaggc
     7981 atgcagaaga tctagtctgc ctcctaattc tcctaagcca gtgagactta ccaaatctaa
     8041 aagttacaca aaaagtgagg agaaaccaag ggatagactt tggtcttctc cagccttctc
     8101 acctacttgc ccatttaggg aagaatctca ggatacatta acccactcat caccttcatt
     8161 taacttagat acactactag tacctaaact agatgttcta agaaacagta tgttcactgc
     8221 tggaaaagga gttgcagaga aagcaagcaa atggtattca agattcacca tgtacaccac
     8281 atcatctaag gatcaaagtt ctgatcgtac cagtctttct tcagtgggag cccaggattc
     8341 tgaatctacc tctttaacag atgaagatgt ctgccatgag ttggaaggac ctatctcctc
     8401 tcaagagacc agtgctactt cagggactaa gagaattgat ctcagccgaa taagcctgga
     8461 aagttctgca tccttggaag gatctctgtc gaagtttgcc ttacctggga aatcagaagt
     8521 gacatcttcc ttcaacgcga gtaatacaaa tatcttccag aactatgcaa tggaggttct
     8581 catctcaagt tgctctcggt gtagaacttg tgattgtctt gtccatgatg aggaaatcat
     8641 ggctggctgg acagcagatg attcaaatct caatactaca tgcccattct gtggcaatat
     8701 cttcttaccc tttctgaata tagaaataag agatttaaga cgacctggaa gatactttct
     8761 aaagtcaagc ccatcaacag aaaatatgca ctttccatcc tccatttcaa gtcagacgag
     8821 gcagtcttgc atttcaacat cagcctctgg tcttgacaca tctgctctct ctgttcaagg
     8881 gaattttgat ctaaatagca aatctaaact gcaggaaaat ttttgcaccc gaagtattca
     8941 gatccctgct aatagatcaa aaacagctat gtctaaatgt ccaatatttc caatggccag
     9001 gagtattagt acttctggcc ccttggataa ggaagatact ggaagacaga agctcatttc
     9061 tacaggcagc ctgccagcta ctttacaagg agctacagat tctttaggtt tagaatggca
     9121 ccttccaagt cccgatcctg tcactgttcc gtatcttagt cctttagtgg tatggaaaga
     9181 acttgaaagc ttattggaaa atgaaggtga tcatgcaata acagtagcag actttgtgga
     9241 ccatcatcca attgtttttt ggaacctggt atggtatttc agacgtcttg acttgcccag
     9301 taacttacct ggattgattc tttcttctga gcactgtaac aagtattcaa agattccccg
     9361 ccactgtatg tctgaagata gcaaatatgt tttaatacaa atgttatggg acaatatgaa
     9421 gttacatcag gatccgggac agcccttgta cattctctgg aatgcacaca cccaaaaata
     9481 tccaatggtc catttattgc aaaaaagtga taactcattt aaccaggaac tgttgaaaag
     9541 tatggtaaaa agcattaaaa tgaatgatgt ctatggacca atgagtcaga ttttagagac
     9601 actgaataag tgtccacatt ttaaaagaca gaggagttta tacagagaaa tactatttct
     9661 ctcacttgtg gcactgggaa gagaaaacat tgatatagat gcttttgata aagagtacaa
     9721 gatggcatac gatcgtctca cacctagtca agtcaagagt acacacaact gtgatagacc
     9781 accaagtaca ggggtgatgg aatgtcgaaa aacctttgga gaaccttatc tttagtaatg
     9841 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     9901 cgacagatga aagcactgta tctgtacacg cgttaagtcg acaatcaacc tctggattac
     9961 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
    10021 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
    10081 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
    10141 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
    10201 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
    10261 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
    10321 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
    10381 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
    10441 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
    10501 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
    10561 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
    10621 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
    10681 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
    10741 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
    10801 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
    10861 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
    10921 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    10981 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    11041 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
    11101 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    11161 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
    11221 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
    11281 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
    11341 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    11401 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    11461 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    11521 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    11581 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    11641 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    11701 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    11761 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    11821 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
    11881 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
    11941 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    12001 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    12061 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    12121 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    12181 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    12241 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    12301 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    12361 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    12421 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    12481 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    12541 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    12601 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    12661 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    12721 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    12781 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    12841 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    12901 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    12961 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    13021 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    13081 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    13141 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    13201 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    13261 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    13321 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    13381 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    13441 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    13501 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    13561 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    13621 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    13681 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    13741 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    13801 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    13861 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    13921 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    13981 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
    14041 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagc
//
