LOCUS       sequence-471138-       12865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12865)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0432
FEATURES             Location/Qualifiers
     source          1..12865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     RBS             8185..8193
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    8722..9310
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8775..8795)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9193..9204)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9382..9615
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9693..9814
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9730..9749)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9784..9803
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9854..9989
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10009..10028)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10010..10028)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10038..10055)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10038..10054)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10047..10069)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10196..10651
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10283..10302)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10493..10514
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10677..10781
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10782..10850,10851..11642)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11000..11019)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11813..12401
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12302..12321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12555..12572
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12689..12710
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12725..12730,12731..12748,12749..12755)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12763..12779
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12768..12790
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12787..12803
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12787..12803
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12822..12842
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12824..12842
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgatg atggcaagaa agcaagatgt
     4141 ccgaattccc acctacaaca tcagtgtggt gggattatct gggaccgaga aggaaaaggg
     4201 ccagtgtggg attggaaagt cttgtttgtg caaccgcttc gtgcgcccga gtgctgacga
     4261 gtttcacttg gaccatacct ccgtcctcag caccagtgac tttggagggc gagtggtcaa
     4321 taatgaccac tttctctact ggggagaagt tagccgctcc ctggaggatt gtgtggaatg
     4381 taagatgcac attgtggagc agactgaatt tattgatgat cagacttttc aacctcatcg
     4441 aagcacggcc ctgcagccct atatcaagag agctgctgcg accaagcttg catcagctga
     4501 aaaactcatg tacttttgca ctgaccagct ggggctggag caggactttg agcagaaaca
     4561 aatgccagac ggaaagctgc tggttgatgg ttttcttctt ggtattgatg ttagcagggg
     4621 catgaatagg aactttgatg accagctcaa gtttgtctcc aatctctaca atcagcttgc
     4681 aaaaacaaaa aagcccatag tggtggtcct gactaagtgt gacgaaggtg ttgagcggta
     4741 cattagagat gcacatactt ttgccttaag caaaaagaac ctccaggttg tggagacctc
     4801 agcgagatcc aatgtaaacg tggacttggc tttcagcacc ttagtgcaac tcattgataa
     4861 aagtcgggga aagacaaaaa tcattcctta ttttgaagct ctcaagcagc agagtcagca
     4921 gatagctaca gcaaaagaca agtatgagtg gctggtgagt cgcattgtga aaaaccacaa
     4981 tgagaactgg ctgagtgtca gccgaaagat gcaggcctct ccagaatacc aggactatgt
     5041 ctacctggaa gggactcaga aagccaagaa gctgtttcta cagcacatcc accgcctcaa
     5101 gcatgagcat atcgagcgta ggagaaagct gtacctggca gccctgccat tagcttttga
     5161 agctcttata cctaatctag atgaaataga ccacctaagc tgcataaaag ccaaaaagct
     5221 cttagaaacc aagccagaat tcttgaagtg gtttgttgtg cttgaagaga ccccatggga
     5281 tgccaccagt cacattgaca acatggaaaa cgaacggatt ccctttgatt taatggatac
     5341 cgtccctgca gagcagctat acgaggccca cttagagaag ctgaggaacg aaaggaaaag
     5401 agttgagatg cgaagggcgt ttaaagaaaa cctggagact tctcctttca taactcccgg
     5461 aaagccttgg gaagaggccc gtagttttat tatgaatgag gatttctacc agtggctgga
     5521 ggaatctgta tacatggata tttatggcaa acaccaaaag caaattatag ataaagcaaa
     5581 ggaagaattt caggagttgc ttttggaata ttcagaattg ttttatgaac tggagctgga
     5641 tgctaagccc agcaaggaga agatgggtgt tattcaggat gttctgggag aggaacagcg
     5701 atttaaagca ttacaaaagc tccaagcaga gcgtgatgcc cttattctga aacacattca
     5761 ttttgtgtac cacccaacaa aggagacatg ccccagctgc ccagcttgtg tggacgctaa
     5821 gattgagcac ttgattagtt ctcggtttat ccggccgtct gaccggaatc agaaaaattc
     5881 actctctgac cctaacattg atagaatcaa cttggttata ttgggcaaag acggccttgc
     5941 ccgagagttg gccaatgaga ttcgagctct ttgtacaaat gatgacaagt atgtgataga
     6001 tggtaaaatg tatgagcttt ccctgaggcc aatagagggg aatgtcaggc ttcctgtgaa
     6061 ctctttccag acgccaacat ttcagcccca cggctgtctc tgcctttaca attcaaagga
     6121 atcgctatcc tatgtagtgg aaagtataga gaagagtaga gagtccacgc tgggccggcg
     6181 ggataatcat ttagtccatc tcccccttac attaattttg gttaacaaga gaggagacac
     6241 cagtggagag actctgcata gcttaataca gcaaggtcaa caaattgcta gcaaacttca
     6301 gtgtgtcttt ctcgaccctg cttctgctgg cattggttac ggacgcaaca ttaatgaaaa
     6361 gcaaatcagt caagttttga agggactcct ggactctaag cgtaacttaa acctggtcag
     6421 ttctactgct agcatcaaag atttggctga tgttgatctg cgaattgtta tgtgtctgat
     6481 gtgtggagat ccttttagtg cagatgacat actttttcct gtccttcagt cccaaacctg
     6541 taaatcttcc cattgtggaa gcaacaactc tgttttactt gaactaccaa tcggactgca
     6601 caagaagcgg attgaactgt ctgttctttc ataccattcc tcctttagca tcagaaagag
     6661 ccggttggtt catgggtaca ttgtttttta ttcagccaaa cgtaaggcct ctttggctat
     6721 gttacgtgcc tttctttgtg aagtgcagga tattatccct attcagcttg tagcactcac
     6781 tgatggcgct gtagatgtcc tggacaatga cttaagtagg gaacagctaa ctgaggggga
     6841 ggagattgct caagaaattg acggaaggtt cacaagcatc ccctgtagcc aaccccagca
     6901 taaacttgag atctttcacc cattttttaa agatgtggtg gaaaaaaaga acataatcga
     6961 ggctactcat atgtacgata atgctgccga ggcctgtagc accaccgaag aggtgtttaa
     7021 ctccccccgg gcaggatcac cgctctgcaa ctcaaacctg caggattcag aagaagatat
     7081 cgagccatct tacagcctgt ttcgagaaga cacatcactg ccttctctgt ccaaagacca
     7141 ttctaagctc tctatggaac tggagggaaa tgatgggctg tctttcatta tgagcaattt
     7201 tgagagtaaa ctgaacaaca aagtacctcc gccagtcaaa ccaaagcctc ctgtccattt
     7261 tgaaattaca aagggggatc tatcttattt agaccaaggc catagggatg gacagaggaa
     7321 gtctgtgtct tctagcccct ggctgcctca ggatgggttt gatccttctg actatgctga
     7381 acccatggat gctgtggtga agccaaggaa tgaagaagaa aacatatact ccgtgcccca
     7441 tgacagcacc caaggcaaaa tcatcaccat tcggaatatc aacaaagccc agtccaacgg
     7501 cagcgggaat ggttctgaca gtgaaatgga caccagctct ctagagcgag ggcgcaaggt
     7561 ttccatcgtg agcaagccag tgctgtacag gacgagatgc acccggctgg ggcggtttgc
     7621 tagttaccgg accagcttca gcgtggggag tgatgatgag ctggggccca tccggaagaa
     7681 agaggaggat caggcatccc agggttataa aggggacaat gctgtcattc catacgaaac
     7741 agacgaagac ccgcggagga ggaatattct tcgcagccta aggaggaaca ctaagaaacc
     7801 aaagcccaaa ccccggccat ccatcacaaa ggcaacctgg gagagtaact attttggggt
     7861 gcccttaaca actgtcgtga ctccagagaa gccgatcccc atttttattg aaagatgtat
     7921 tgagtacatt gaagccacag gactgagcac ggaaggcatc taccgggtca gcgggaacaa
     7981 gtctgagatg gagagtctgc agagacagtt tgatcaagac cacaacctgg acctggcaga
     8041 gaaagacttt acggtgaata ccgtggctgg tgccatgaag agctttttct cagaactgcc
     8101 tgaccccctg gtcccgtata acatgcagat cgacttggtg gaagcacaca aaatcaacga
     8161 ccgggagcag aagttgcatg cccttaagga ggtattaaag aaatttccaa aggaaaacca
     8221 cgaagtcttc aagtatgtca tctctcacct aaacaaggtc agccacaaca acaaggtgaa
     8281 tctcatgacc agcgagaacc tctccatctg cttctggccc accttgatga gacctgattt
     8341 cagcactatg gacgccctca cagccacgcg cacctaccag acaatcattg aactctttat
     8401 ccagcagtgc cccttcttct tctacaatcg gcccatcacc gagccccccg gcgccaggcc
     8461 cagctccccc tctgccgtgg cttccaccgt ccccttcctc acttccacgc ctgtcacaag
     8521 tcagccgtcg cccccacagt cgcctccacc caccccccag tccccaatgc agccactgct
     8581 tccctcccag cttcaagccg aacacacgct gtagtaatga actagtccgt aacttgaaag
     8641 tatttcgatt tcttggcttt atatatcttg tggaaaggac gactaagcat tgtttactga
     8701 atctggacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     8761 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     8821 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     8881 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     8941 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     9001 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     9061 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     9121 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     9181 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     9241 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     9301 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     9361 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     9421 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     9481 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     9541 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     9601 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     9661 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     9721 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     9781 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     9841 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     9901 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     9961 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
    10021 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
    10081 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    10141 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
    10201 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    10261 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    10321 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    10381 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    10441 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    10501 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    10561 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    10621 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
    10681 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    10741 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
    10801 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    10861 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    10921 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    10981 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
    11041 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    11101 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    11161 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    11221 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    11281 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    11341 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    11401 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    11461 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    11521 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    11581 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    11641 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    11701 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    11761 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    11821 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    11881 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    11941 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    12001 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    12061 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    12121 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    12181 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    12241 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    12301 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    12361 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    12421 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    12481 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    12541 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    12601 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    12661 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    12721 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    12781 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
    12841 gggaacaaaa gctggagctg caagc
//
