LOCUS       sequence-471156-       13435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13435)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0951
FEATURES             Location/Qualifiers
     source          1..13435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    9292..9880
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9345..9365)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9763..9774)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9952..10185
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10263..10384
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10300..10319)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10354..10373
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10424..10559
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10579..10598)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10580..10598)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10608..10625)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10608..10624)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10617..10639)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10766..11221
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10853..10872)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11063..11084
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11247..11351
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11352..11420,11421..12212)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11570..11589)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12383..12971
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12872..12891
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13125..13142
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13259..13280
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13295..13300,13301..13318,13319..13325)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13333..13349
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13338..13360
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13357..13373
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13357..13373
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13392..13412
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13394..13412
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgggt tccaagagaa gaagagctac
     4141 ctccccttcc agcagtgtca gcggggactt tgatgatggg caccattctg tgtcaacacc
     4201 aggcccaagc aggaaaagga ggagactttc caatcttcca actgtagatc ctattgccgt
     4261 gtgccatgaa ctctataata ccatccgaga ctataaggat gaacagggca gacttctctg
     4321 tgagctcttc attagggcac caaagcgaag aaatcaacca gactattatg aagtggtttc
     4381 tcagcccatt gacttgatga aaatccaaca gaaactaaaa atggaagagt atgatgatgt
     4441 taatttgctg actgctgact tccagcttct ttttaacaat gcaaagtcct attataagcc
     4501 agattctcct gaatataaag ccgcttgcaa actctgggat ttgtaccttc gaacaagaaa
     4561 tgagtttgtt cagaaaggag aagcagatga cgaagatgat gatgaagatg ggcaagacaa
     4621 tcagggcaca gtgactgaag gatcttctcc agcttacttg aaggagatcc tggagcagct
     4681 tcttgaagcc atagttgtag ctacaaatcc atcaggacgt ctcattagcg aactttttca
     4741 gaaactgcct tctaaagtgc aatatccaga ttattatgca ataattaagg agcctataga
     4801 tctcaagacc attgcccaga ggatacagaa tggaagctac aaaagtattc atgcaatggc
     4861 caaagatata gatctcctcg caaaaaatgc caaaacttat aatgagcctg gctctcaagt
     4921 attcaaggat gcaaattcaa ttaaaaaaat attttatatg aaaaaggctg aaattgaaca
     4981 tcatgaaatg gctaagtcaa gtcttcgaat gaggactcca tccaacttgg ctgcagccag
     5041 actgacaggt ccttcacaca gtaaaggcag ccttggtgaa gagagaaatc ccactagcaa
     5101 gtattaccgt aataaaagag cagtacaagg aggtcgttta tcagcaatta caatggcact
     5161 tcaatatggc tcagaaagtg aagaagatgc tgctttagct gctgcacgct atgaagaggg
     5221 agagtcagaa gcagaaagca tcacttcctt tatggatgtt tcaaatcctt tttatcagct
     5281 ttatgacaca gttaggagtt gtcggaataa ccaagggcag ctaatagctg aaccttttta
     5341 ccatttgcct tcaaagaaaa aataccctga ttattaccag caaattaaaa tgcccatatc
     5401 actacaacag atccgaacaa aactgaagaa tcaagaatat gaaactttag atcatttgga
     5461 gtgtgatctg aatttaatgt ttgaaaatgc caaacgctat aatgtgccca attcagccat
     5521 ctacaagcga gttctaaaat tgcagcaagt tatgcaggca aagaagaaag agcttgccag
     5581 gagagacgat atcgaggacg gagacagcat gatctcttca gccacctctg atactggtag
     5641 tgccaaaaga aaaagtaaaa agaacataag aaagcagcga atgaaaatct tattcaatgt
     5701 tgttcttgaa gctcgagagc caggttcagg cagaagactt tgtgacctat ttatggttaa
     5761 accatccaaa aaggactatc ctgattatta taaaatcatc ttggagccaa tggacttgaa
     5821 aataattgag cataacatcc gcaatgacaa atatgctggt gaagagggaa tgatagaaga
     5881 catgaagctg atgttccgga atgccaggca ctataatgag gagggctccc aggtttataa
     5941 tgatgcacat atcctggaga agttactcaa ggagaaaagg aaagagctgg gcccactgcc
     6001 tgatgatgat gacatggctt ctcccaaact caagctgagt aggaagagtg gcatttctcc
     6061 taaaaaatca aaatacatga ctccaatgca gcagaaacta aatgaggtct atgaagctgt
     6121 aaagaactat actgataaga ggggtcgccg cctcagtgcc atatttctga ggcttccctc
     6181 tagatctgag ttgcctgact actatctgac tattaaaaag cccatggaca tggaaaaaat
     6241 tcgaagtcac atgatggcca acaagtacca agatattgac tctatggttg aggactttgt
     6301 catgatgttt aataatgcct gtacatacaa tgagccggag tctttgatct acaaagatgc
     6361 tcttgttcta cacaaagtcc tgcttgaaac acgcagagac ctggagggag atgaggactc
     6421 tcatgtccca aatgtgactt tgctgattca agagcttatc cacaatcttt ttgtgtcagt
     6481 catgagtcat caggatgatg agggaagatg ctacagcgat tctttagcag aaattcctgc
     6541 tgtggatccc aactttccta acaaaccacc ccttacattt gacataatta ggaagaatgt
     6601 tgaaaataat cgctaccgtc ggcttgattt atttcaagag catatgtttg aagtattgga
     6661 acgagcaaga aggatgaatc ggacagattc agaaatatat gaagatgcag tagaacttca
     6721 gcagtttttt attaaaattc gtgatgaact ctgcaaaaat ggagagattc ttctttcacc
     6781 ggcactcagc tataccacaa aacatttgca taatgatgtg gagaaagaga gaaaggaaaa
     6841 attgccaaaa gaaatagagg aagataaact aaaacgagaa gaagaaaaaa gagaagctga
     6901 aaagagtgaa gattcctctg gtgctgcagg cctctcaggc ttacatcgca catacagcca
     6961 ggactgtagc tttaaaaaca gcatgtacca tgttggagat tacgtctatg tggaacctgc
     7021 agaggccaac ctacaaccac atatcgtctg tattgaaaga ctgtgggagg attcagctgg
     7081 tgaaaaatgg ttgtatggct gttggtttta ccgaccaaat gaaacattcc acctggctac
     7141 acgaaaattt ctagaaaaag aagtttttaa gagtgactat tacaacaaag ttccagttag
     7201 taaaattcta ggcaagtgtg tggtcatgtt tgtcaaggaa tactttaagt tatgcccaga
     7261 aaacttccga gatgaggatg tttttgtctg tgaatcacgg tattctgcca aaaccaaatc
     7321 ttttaagaaa attaaactgt ggaccatgcc catcagctca gtcaggtttg tccctcggga
     7381 tgtgcctctg cctgtggttc gcgtggcctc tgtatttgca aatgcagata aaggtgatga
     7441 tgagaagaat acagacaact cagaggacag tcgagctgaa gacaatttta acttggaaaa
     7501 ggaaaaagaa gatgtccctg tggaaatgtc caatggtgaa ccaggttgcc actactttga
     7561 gcagctccat tacaatgaca tgtggctgaa ggttggcgac tgtgtcttca tcaagtccca
     7621 tggcctggtg cgtcctcgtg tgggcagaat tgaaaaagta tgggttcgag atggagctgc
     7681 atatttttat ggccccatct tcattcaccc agaagaaaca gagcatgagc ccacaaaaat
     7741 gttctacaaa aaagaagtat ttctgagtaa tctggaagaa acctgcccca tgacatgtat
     7801 tctcggaaag tgtgctgtgt tgtcattcaa ggacttcctc tcctgcaggc caactgaaat
     7861 accagaaaat gacattctgc tttgtgagag ccgctacaat gagagcgaca agcagatgaa
     7921 gaaattcaaa ggattgaaga ggttttcact ctctgctaaa gtggtagatg atgaaattta
     7981 ctacttcaga aaaccaattg ttcctcagaa ggagccatca cctttgctgg aaaagaagat
     8041 ccagttgcta gaagctaaat ttgccgagtt agaaggtgga gatgatgata ttgaagagat
     8101 gggagaagaa gatagtgagg tcattgaacc tccttctcta cctcagcttc agacccccct
     8161 ggccagtgag ctggacctca tgccctacac acccccacag tctaccccaa agtctgccaa
     8221 aggcagtgca aagaaggaag gctccaaacg gaaaatcaac atgagtggct acatcctgtt
     8281 cagcagtgag atgagggctg tgattaaggc ccaacaccca gactactctt tcggggagct
     8341 cagccgcctg gtggggacag aatggagaaa tcttgagaca gccaagaaag cagaatatga
     8401 agagcgggca gctaaagttg ctgagcagca ggagagagag cgagcagcac agcaacagca
     8461 gccgagtgct tctccccgag caggcacccc tgtgggggct ctcatggggg tggtgccacc
     8521 accaacacca atggggatgc tcaatcagca gttgacacct gttgcaggca tgatgggtgg
     8581 ctatccgcca ggccttccac ctttgcaggg cccagttgat ggccttgtta gcatgggcag
     8641 catgcagcca cttcaccctg gggggcctcc accccaccat cttccgccag gtgtgcctgg
     8701 cctcccgggc atcccaccac cgggtgtgat gaaccaagga gtggccccta tggtagggac
     8761 tccagcacca ggtggaagtc catatggaca acaggtggga gttttggggc ctccagggca
     8821 gcaggcacca cctccatatc ccggcccaca tccagctgga ccccctgtca tacagcagcc
     8881 aacaacaccc atgtttgtag ctcccccacc aaagacccag cggcttcttc actcagaggc
     8941 ctacctgaaa tacattgaag gactcagtgc ggagtccaac agcattagca agtgggatca
     9001 gacactggca gctcgaagac gcgacgtcca tttgtcgaaa gaacaggaga gccgcctacc
     9061 ctctcactgg ctgaaaagca aaggggccca caccaccatg gcagatgccc tctggcgcct
     9121 tcgagatttg atgctccggg acaccctcaa cattcgccaa gcatacaacc tagaaaatgt
     9181 ttagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     9241 tggaaaggac gatgcgagtc ctagatgact atacctacgc gttaagtcga caatcaacct
     9301 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     9361 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     9421 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     9481 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     9541 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     9601 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     9661 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     9721 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     9781 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     9841 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     9901 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     9961 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
    10021 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
    10081 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    10141 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
    10201 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
    10261 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    10321 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    10381 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
    10441 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    10501 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    10561 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
    10621 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
    10681 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
    10741 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
    10801 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    10861 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    10921 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    10981 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    11041 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    11101 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
    11161 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
    11221 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    11281 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    11341 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    11401 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
    11461 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
    11521 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
    11581 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    11641 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    11701 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    11761 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    11821 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    11881 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    11941 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    12001 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    12061 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    12121 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    12181 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    12241 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    12301 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    12361 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    12421 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    12481 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    12541 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    12601 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    12661 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    12721 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    12781 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    12841 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    12901 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    12961 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    13021 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    13081 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    13141 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    13201 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    13261 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    13321 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    13381 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagc
//
