LOCUS       sequence-471158-       12463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12463)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1777
FEATURES             Location/Qualifiers
     source          1..12463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6778..6789
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    8320..8908
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8373..8393)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8791..8802)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8980..9213
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9291..9412
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9328..9347)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9382..9401
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9452..9587
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9607..9626)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9608..9626)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9636..9653)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9636..9652)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9645..9667)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9794..10249
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9881..9900)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10091..10112
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10275..10379
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10380..10448,10449..11240)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10598..10617)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11411..11999
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11900..11919
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12153..12170
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12287..12308
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12323..12328,12329..12346,12347..12353)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12361..12377
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12366..12388
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12385..12401
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12385..12401
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12420..12440
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12422..12440
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggaa tttagcatca agcagagtcc
     4141 cctttctgtt cagagtgttg taaagtgcat aaagatgaag caggcaccag aaatcctcgg
     4201 cagtgccaac gggaagactc cgagctgcga ggtgaaccgc gagtgttctg tgttcctcag
     4261 caaagcccag ctctccagta gcctgcagga gggggtcatg cagaagttta acggccacga
     4321 cgccctgccc tttattccag ccgacaagct gaaagatctt acttcccggg tgtttaatgg
     4381 agaacccggc gcacacgatg ccaaactgcg ttttgagtcc caggaaatga aagggattgg
     4441 gacaccccct aacactaccc ctatcaaaaa tggctctcca gaaattaagc tgaaaatcac
     4501 caaaacatac atgaatggga agcctctctt tgaatcttcc atttgtggtg acagtgctgc
     4561 tgatgtgtct cagtcagaag aaaatggaca aaaaccagaa aacaaggcga gaaggaacag
     4621 gaagaggagc ataaaatatg actccttgct ggagcagggc cttgtcgaag cagctcttgt
     4681 gtctaagatc tcaagtcctt cagataaaaa gattccagct aagaaagagt cttgtccaaa
     4741 cactggaaga gacaaagacc acctgttgaa atacaacgtt ggtgatttgg tgtggtccaa
     4801 agtgtcgggt tacccttggt ggccttgcat ggtttctgca gatccactcc ttcacagcta
     4861 taccaaactt aaaggtcaga aaaagagtgc acgccagtat cacgtacagt tctttggtga
     4921 cgccccagaa agagcttgga tatttgagaa gagcctcgta gcttttgaag gagaaggaca
     4981 gtttgaaaaa ttatgccagg aaagtgccaa gcaggcaccc acgaaagctg agaaaattaa
     5041 gctattgaaa ccaatttcag ggaaattgag ggcccagtgg gaaatgggca ttgttcaagc
     5101 agaagaagct gcaagcatgt cagtggagga gcggaaagcc aagttcacct ttctctatgt
     5161 gggggaccag cttcatctca accctcaagt agccaaggag gctggcattg ctgcagagtc
     5221 tttgggagaa atggcagaat cctcaggagt cagtgaagaa gctgctgaaa accccaagtc
     5281 tgtgagagaa gagtgcattc ccatgaagag aaggcggagg gccaaactgt gtagctctgc
     5341 agagaccctg gagagtcacc ccgacatagg gaagagtact cctcaaaaga cggcagaggc
     5401 tgaccccaga agaggagtag ggtctcctcc tgggaggaag aagaccacag tctccatgcc
     5461 acgaagcagg aagggagatg cagcatccca gtttttggtc ttctgtcaaa aacacaggga
     5521 tgaggtggta gctgagcacc cagatgcttc aggtgaggag attgaagagc tgctcaggtc
     5581 acagtggagt ctgctgagtg agaagcagag agcacgctac aacaccaagt ttgccctggt
     5641 ggcccctgtc caggctgaag aagactctgg taatgtaaat gggaaaaaaa gaaaccacac
     5701 aaagaggata caggacccta cagaagatgc tgaagctgag gacacaccca ggaaaagact
     5761 caggacggac aagcacagtc ttcggaagag agacacaatc actgacaaaa cggccagaac
     5821 aagctcttac aaggccatgg aggcagcctc ctcgctcaag agccaggcag caacgaaaaa
     5881 tctgtctgat gcatgtaaac cactgaagaa gcgaaatcgg gcttccacgg cagcatcttc
     5941 agctcttggg tttagcaaaa gttcatctcc ttctgcatcc ttaactgaga atgaggtctc
     6001 ggacagcccg ggagacgagc cctcggagtc cccatacgaa agtgcagacg aaacacaaac
     6061 tgaagtatct gtctcatcca aaaagtctga gcgaggagtg actgccaaaa aggagtatgt
     6121 gtgccagctg tgtgagaagc cgggcagcct cctgctctgt gaaggaccct gctgcggagc
     6181 tttccacctc gcctgccttg ggctttcccg gaggccagaa gggaggttca cctgcagcga
     6241 gtgtgcctca gggattcact catgtttcgt gtgtaaagag agcaagacag atgttaagcg
     6301 ctgtgtggta actcagtgtg gaaaatttta ccatgaggct tgtgtgaaaa aataccctct
     6361 gactgtattt gagagccgag gtttccgctg ccccctccac agctgtgtga gctgccatgc
     6421 ttccaaccct tcaaacccaa ggccgtcaaa aggtaaaatg atgcggtgtg tccgctgccc
     6481 cgttgcctat cacagcgggg atgcttgtct ggcagcagga tgctcagtga tcgcctccaa
     6541 cagcatcatc tgcactgccc acttcactgc tcggaagggg aagcgacacc acgcccacgt
     6601 caacgtgagc tggtgcttcg tgtgctccaa aggggggagc cttctgtgct gtgagtcctg
     6661 cccagcggcc ttccaccctg actgcctgaa catcgagatg cctgacggca gctggttctg
     6721 caatgactgc agggctggga agaagctgca cttccaggat atcatttggg tgaaacttgg
     6781 gaactacaga tggtggccgg cagaagtttg ccatcccaaa aatgttcccc caaatattca
     6841 gaaaatgaag cacgagattg gagaattccc tgtgtttttc tttgggtcta aagattatta
     6901 ctggacgcat caggcgcgag tgttcccgta catggagggg gaccggggca gccgctacca
     6961 gggggtcaga gggatcggaa gagtcttcaa aaacgcactg caagaagctg aagctcgttt
     7021 tcgtgaaatt aagcttcaga gggaagcccg agaaacacag gagagcgagc gcaagccccc
     7081 accatacaag cacatcaagg tgaataagcc ttacgggaaa gtccagatct acacagcgga
     7141 tatttcagaa atccctaagt gcaactgcaa gcccacagat gagaatcctt gtggctttga
     7201 ttcggagtgt ctgaacagga tgctgatgtt tgagtgccac ccgcaggtgt gtcccgcggg
     7261 cgagttctgc cagaaccagt gcttcaccaa gcgccagtac ccagagacca agatcatcaa
     7321 gacagatggc aaagggtggg gcctggtcgc caagagggac atcagaaagg gagaatttgt
     7381 taacgagtac gttggggagc tgatcgacga ggaggagtgc atggcgagaa tcaagcacgc
     7441 acacgagaac gacatcaccc acttctacat gctcactata gacaaggacc gtataataga
     7501 cgctggcccc aaaggaaact actctcgatt tatgaatcac agctgccagc ccaactgtga
     7561 gaccctcaag tggacagtga atggggacac tcgtgtgggc ctgtttgccg tctgtgacat
     7621 tcctgcaggg acggagctga cttttaacta caacctcgat tgtctgggca atgaaaaaac
     7681 ggtctgccgg tgtggagcct ccaattgcag tggattcctc ggggatagac caaagacctc
     7741 gacgaccctt tcatcagagg aaaagggcaa aaagaccaag aagaaaacga ggcggcgcag
     7801 agcaaaaggg gaagggaaga ggcagtcaga ggacgagtgc ttccgctgcg gtgatggcgg
     7861 gcagctggtg ctgtgtgacc gcaagttctg caccaaggcc taccacctgt cctgcctggg
     7921 ccttggcaag cggcccttcg ggaagtggga atgtccttgg catcattgtg acgtgtgtgg
     7981 caaaccttcg acttcatttt gccacctctg ccccaattcg ttctgtaagg agcaccagga
     8041 cgggacagcc ttcagctgca ccccggacgg gcggtcctac tgctgtgagc atgacttagg
     8101 ggcggcatcg gtcagaagca ccaagactga gaagcccccc ccagagccag ggaagccgaa
     8161 ggggaagagg cggcggcgga ggggctggcg gagagtcaca gagggcaaat agtaatgaac
     8221 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     8281 taatcttagc gtcacttctt ggaaacgcgt taagtcgaca atcaacctct ggattacaaa
     8341 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     8401 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     8461 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     8521 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     8581 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     8641 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     8701 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     8761 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     8821 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     8881 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     8941 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     9001 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     9061 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     9121 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     9181 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     9241 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     9301 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     9361 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     9421 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     9481 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     9541 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     9601 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     9661 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     9721 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     9781 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     9841 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     9901 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     9961 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
    10021 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    10081 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
    10141 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
    10201 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
    10261 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    10321 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    10381 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    10441 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    10501 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    10561 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    10621 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    10681 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    10741 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    10801 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    10861 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    10921 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    10981 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    11041 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    11101 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    11161 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    11221 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    11281 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    11341 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    11401 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    11461 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    11521 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    11581 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    11641 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    11701 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    11761 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    11821 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    11881 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    11941 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    12001 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    12061 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    12121 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    12181 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    12241 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    12301 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    12361 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
    12421 caattaaccc tcactaaagg gaacaaaagc tggagctgca agc
//
