LOCUS       sequence-471161-       13903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13903)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1784
FEATURES             Location/Qualifiers
     source          1..13903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    9760..10348
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9813..9833)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10231..10242)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10420..10653
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10731..10852
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10768..10787)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10822..10841
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10892..11027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11047..11066)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11048..11066)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11076..11093)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11076..11092)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11085..11107)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11234..11689
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11321..11340)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11531..11552
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11715..11819
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11820..11888,11889..12680)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12038..12057)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12851..13439
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13340..13359
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13593..13610
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13727..13748
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13763..13768,13769..13786,13787..13793)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13801..13817
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13806..13828
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13825..13841
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13825..13841
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13860..13880
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13862..13880
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgggc ctttgtgggc tgctggagag
     4141 atgttggctg caccatgacc cagatggagt tttaacattg aatgcggaga acactaatta
     4201 tgcctatcaa gttccaaact tccataaatg tgaaatctgt ctactatctt ttccaaaaga
     4261 atcccagttt caacgccaca tgagggatca cgagcgaaat gacaagccac atcgatgtga
     4321 ccagtgcccc caaacattta atgttgaatt caacctgaca cttcataaat gcacccacag
     4381 cggggaagat cctacctgcc ctgtgtgtaa caagaaattc tccagagtgg ctagtctcaa
     4441 agcgcatatt atgctacatg aaaaggaaga gaatctcatc tgttctgagt gtggggatga
     4501 gtttactctg cagagtcagc tggccgtgca catggaggag caccgccagg agctggctgg
     4561 aacccggcag catgcctgca aggcctgcaa gaaagagttc gagacctcct cggagctgaa
     4621 ggaacacatg aagactcatt acaaaattag ggtatcaagt acaaggtctt ataaccggaa
     4681 tatcgacaga agtggattca cgtattcgtg tccgcactgt ggaaagacgt ttcaaaagcc
     4741 aagccagtta acgcgacaca ttaggataca cacaggtgaa aggccgttca aatgtagtga
     4801 atgtggaaag gcttttaacc agaagggggc actgcagacc cacatgatca agcacacagg
     4861 tgaaaaaccc catgcctgtg ccttctgtcc tgccgccttc tctcagaaag ggaatcttca
     4921 gtcgcacgtg cagcgagtcc actcagaggt caagaatggt cctacctata actgtacaga
     4981 atgtagttgt gtatttaaaa gtttaggcag cttaaacacg catatcagca agatgcatat
     5041 gggtgggcca cagaattcaa caagttctac agagactgct catgttttaa cggccacact
     5101 ttttcagacg ttacctcttc aacagacgga agcccaagcc acgtcggcct caagccagcc
     5161 gagctcccag gcggtgagcg acgtcatcca gcagctcctg gagctctcag agccggcgcc
     5221 ggtggagtcg gggcagtccc cgcagcctgg gcagcagctg agcatcacag tgggcatcaa
     5281 ccaggacatt ttacagcaag ccttagaaaa cagtgggctg tcttcaattc cagctgcagc
     5341 acatcctaat gactcctgcc atgccaagac ctctgcacca cacgctcaaa acccagatgt
     5401 ttccagcgtt tcaaatgagc agacggaccc cacagacgca gagcaagaaa aagaacagga
     5461 aagcccggag aaactggata aaaaagaaaa aaaaatgata aagaagaagt caccgtttct
     5521 acctggctcc atccgcgagg agaacggcgt gcgctggcat gtgtgtccct actgcgccaa
     5581 ggagttccgc aagcccagcg acctggtccg ccacatccgc atccacaccc acgagaagcc
     5641 cttcaagtgc ccgcagtgct tccgcgcctt cgccgtgaag agcacgctga cagcgcacat
     5701 caagacgcac accggcatca aggcgttcaa gtgccagtac tgcatgaaga gcttctccac
     5761 ctctggcagc ctcaaggtgc acattcgcct gcacacagga gttagacctt ttgcttgtcc
     5821 tcactgtgac aaaaaatttc gaacctcagg ccataggaag actcacattg cttcccactt
     5881 taaacatacg gaattaagga aaatgaggca ccagcgtaaa cctgcaaagg tccgtgttgg
     5941 caagacgaat attccagtcc ctgatattcc tttgcaggaa ccaatcctca taactgactt
     6001 aggtctcatc cagcccattc caaaaaacca gtttttccaa agctatttca ataataattt
     6061 tgtcaatgaa gcagatagac catacaagtg tttttactgt catcgtgcat ataaaaaatc
     6121 ttgccacctt aaacaacaca tcagatccca tacaggtgaa aaacctttta aatgttctca
     6181 gtgtggaaga ggctttgttt ctgcaggcgt gctcaaagca cacatcagaa cacacacagg
     6241 actgaaatct ttcaagtgtc tgatatgtaa tggggctttc actactggtg gcagcttacg
     6301 gcgacacatg ggtatccaca acgaccttcg tccctatatg tgtccctatt gccaaaaaac
     6361 atttaagact tcactaaatt gcaaaaagca catgaaaacc cacagatatg agcttgccca
     6421 gcagctccaa cagcatcagc aggcagcctc gatagatgac agcactgtag accagcagag
     6481 catgcaggcc tccactcaaa tgcaggtgga gatcgagagc gacgagctgc cgcagacggc
     6541 agaggtggtc gcagcgaacc ccgaggccat gctggacctg gagcctcagc atgtggtggg
     6601 cacggaggaa gcagggctgg gccagcagtt ggcagatcag cccctggaag cagatgaaga
     6661 tgggtttgtg gctccacagg accctctgcg agggcacgta gaccagtttg aagagcagag
     6721 ccctgcgcaa cagtccttcg aaccagcagg gctaccccaa ggttttacag tgactgatac
     6781 gtaccatcag cagcctcagt ttccacctgt ccaacagcta caggattcca gcacacttga
     6841 gtctcaggcc ctctccacaa gcttccacca gcagagcttg ctgcaggctc ccagctctga
     6901 tgggatgaat gtaacaactc gcttgattca ggagtcatcc caagaggaac tggacctgca
     6961 ggcacaaggt tcccagtttc tggaggacaa cgaggaccag agcaggcgct cttacaggtg
     7021 tgactattgc aacaaaggct ttaagaagtc cagccacctg aagcagcatg tgcggtcgca
     7081 caccggggaa aagccctaca agtgcaagct ctgtggacgc ggctttgttt cctctggggt
     7141 cctcaagtcc cacgagaaga cacacacagg agtgaaggcg ttcagctgca gtgtgtgcaa
     7201 tgcttccttc accaccaatg gcagcctcac ccggcacatg gccacacata tgagcatgaa
     7261 gccttataag tgtccgtttt gtgaggaggg tttccgaact acagtgcatt gtaaaaagca
     7321 catgaagaga caccaaacag tcccctctgc tgtgtcagcc actggagaga cagaaggagg
     7381 agacatttgt atggaggaag aggaagaaca ttctgacaga aatgcatcac ggaagtctcg
     7441 tcctgaggtc atcactttca cggaggagga gacagcccag ttagccaaga tccggccgca
     7501 ggagagcgcc acggtgtcag agaaggtcct ggtgcagtcc gcggcagaaa aggaccgcat
     7561 cagtgagctg agggacaagc aggcggagct gcaggacgag cccaagcacg ccaactgctg
     7621 cacatactgc cccaagagct tcaagaaacc tagcgacctg gtgaggcatg ttcgaatcca
     7681 tactggagaa aagccataca aatgtgatga atgtggaaag agttttactg tgaaatccac
     7741 tctcgattgt catgtgaaga ctcacacagg tcagaagctc ttcagctgtc acgtctgcag
     7801 caacgccttc tccacgaagg gaagtctgaa ggtccacatg cgcctgcaca cgggagccaa
     7861 gcccttcaaa tgcccgcatt gcgagctgcg tttccgtacc tcgggtagaa gaaagacaca
     7921 catgcagttt cattataaac cagacccaaa gaaggccaga aagcctatga ctcgaagctc
     7981 atcggaagga ctgcagcctg taaacctcct caactcctcc tctactgacc caaacgtgtt
     8041 tatcatgaac aactctgttc taacaggaca gtttgatcag aatctgctgc aaccaggact
     8101 ggtgggccaa gctattctcc ctgcctctgt gtcagctggg ggtgacctga ccgtgtctct
     8161 gacagatggg agcctggcta ccctagaagg catccagtta cagttggctg ctaacttggt
     8221 tggaccaaat gtacagattt ctggaatcga tgctgccagc attaataaca ttacgttgca
     8281 gattgatcca agcattctgc agcagacgct acagcagggc aacctattgg ctcagcagct
     8341 cacgggggag cctggcctgg ccccacagaa cagctctctc cagacatcgg acagcacggt
     8401 ccctgccagt gttgtcatcc agcccatctc aggcctgtcc ttacagccca cagtgacctc
     8461 tgcgaacctg accataggcc cgctgtctga gcaggattca gtgctgacca ctaacagcag
     8521 tgggacccaa gacctcactc aagtgatgac ttcgcaaggt ctagtgtccc cctccggcgg
     8581 tccccacgag atcaccctga ccattaacaa ctccagcctg agccaggtcc tggcacaggc
     8641 cgctgggccc actgccacgt cttcctcggg gtctccacag gaaattaccc tgactatctc
     8701 cgaacttaac actacaagcg gaagccttcc ttcaacaaca ccgatgtctc catcggccat
     8761 ctcgactcag aacctggtca tgtcctcgtc gggcgtggga ggtgacgcta gtgtcacgct
     8821 gacgctggcc gatactcagg gtatgctatc tggaggcctg gacactgtca cactcaacat
     8881 cacctctcag ggtcagcagt tcccagcgct cctcacggat ccctctctct cgggccaggg
     8941 tggagcaggc tcgccgcaag tcatactagt gagccacacg ccacagtcag cgtctgctgc
     9001 ttgtgaagaa atagcctacc aggtagctgg cgtctctggg aacctggccc cgggcaacca
     9061 gccagagaag gagggccggg cgcaccagtg cctggagtgt gaccgcgcct tctcatcggc
     9121 ggcggtgctc atgcaccaca gcaaggaggt gcatggccgg gagcgcatcc acggctgccc
     9181 cgtgtgcagg aaggccttca agcgcgccac gcacctcaag gagcacatgc agacacacca
     9241 ggccggcccc tctttgagct cccagaagcc aagagtgttt aaatgtgaca cttgtgagaa
     9301 ggcatttgcc aaaccaagcc agctggagcg ccacagccgc atacatacag gggagcggcc
     9361 gttccattgc acgctttgtg agaaagcctt caaccagaag agtgcgctgc aggtgcacat
     9421 gaagaagcac acgggggagc ggccctacaa gtgtgcctac tgcgtcatgg gcttcacgca
     9481 gaagagcaac atgaagctgc acatgaagcg ggcgcacagc tatgctggag ctctgcagga
     9541 gtctgcaggt cacccggagc aggacgggga ggagctgagc cggaccctcc acctggagga
     9601 ggtggtgcag gaggccgccg gcgagtggca ggccctcacc cacgtcttct agtaatgaac
     9661 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     9721 catgtggcca tgaggatttg cgatacgcgt taagtcgaca atcaacctct ggattacaaa
     9781 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     9841 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     9901 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     9961 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
    10021 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
    10081 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
    10141 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
    10201 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
    10261 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
    10321 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
    10381 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
    10441 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
    10501 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
    10561 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
    10621 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
    10681 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
    10741 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    10801 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    10861 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
    10921 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
    10981 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
    11041 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
    11101 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
    11161 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
    11221 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
    11281 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
    11341 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
    11401 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
    11461 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    11521 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
    11581 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
    11641 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
    11701 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    11761 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    11821 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    11881 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    11941 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    12001 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    12061 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    12121 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    12181 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    12241 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    12301 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    12361 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    12421 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    12481 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    12541 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    12601 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    12661 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    12721 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    12781 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    12841 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    12901 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    12961 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    13021 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    13081 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    13141 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    13201 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    13261 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    13321 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    13381 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    13441 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    13501 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    13561 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    13621 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    13681 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    13741 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    13801 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
    13861 caattaaccc tcactaaagg gaacaaaagc tggagctgca agc
//
