LOCUS       sequence-471162-       14293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14293)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 14293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2037
FEATURES             Location/Qualifiers
     source          1..14293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    10150..10738
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10203..10223)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10621..10632)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10810..11043
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11121..11242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11158..11177)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11212..11231
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11282..11417
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11437..11456)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11438..11456)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11466..11483)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11466..11482)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11475..11497)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11624..12079
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11711..11730)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11921..11942
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12105..12209
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12210..12278,12279..13070)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12428..12447)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13241..13829
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13730..13749
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13983..14000
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14117..14138
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14153..14158,14159..14176,14177..14183)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14191..14207
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14196..14218
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14215..14231
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14215..14231
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14250..14270
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14252..14270
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgcct ggggagcaga tggaccctac
     4141 tggaagtcag ttggattcag atttctctca gcaagatact ccttgcctga taattgaaga
     4201 ttctcagcct gaaagccagg ttctagagga tgattctggt tctcacttca gtatgctatc
     4261 tcgacacctt cctaatctcc agacgcacaa agaaaatcct gtgttggatg ttgtgtccaa
     4321 tcctgaacaa acagctggag aagaacgagg agacggtaat agtgggttca atgaacattt
     4381 gaaagaaaac aaggttgcag accctgtgga ttcttctaac ttggacacat gtggttccat
     4441 cagtcaggtc attgagcagt tacctcagcc aaacaggaca agcagtgttc tgggaatgtc
     4501 agtggaatct gctcctgctg tggaggaaga gaagggagaa gagttggaac agaaggagaa
     4561 agagaaggaa gaagatactt caggcaatac tacacattcc cttggtgctg aagatactgc
     4621 ctcatcacag ttgggttttg gggttctgga actctcccag agccaggatg ttgaggaaaa
     4681 tactgtgcca tatgaagtgg acaaagagca gctacaatca gtaaccacca actctggtta
     4741 taccaggctg tctgatgtgg atgctaatac tgcaattaag catgaagaac agtccaacga
     4801 agatatcccc atagcagaac agtccagcaa ggacatccct gtgacagcac agcccagtaa
     4861 ggatgtacat gttgtaaaag agcaaaatcc accacctgca aggtcagagg acatgccttt
     4921 tagccccaaa gcatctgttg ctgctatgga agcaaaagaa cagttgtctg cacaagaact
     4981 tatggaaagt ggactgcaga ttcagaagtc accagagcct gaggttttgt caactcagga
     5041 agacttgttt gaccagagca ataaaacagt atcttctgat ggttgctcta ctccttcaag
     5101 ggaggaaggt gggtgttctt tggcttccac tcctgccacc actctgcatc tcctgcagct
     5161 ctctggtcag aggtcccttg ttcaggacag tctttccacg aattcttcag atcttgttgc
     5221 tccttctcct gatgctttcc gatctactcc ttttatcgtt cctagcagtc ccacagagca
     5281 agaagggaga caagataagc caatggacac gtcagtgtta tctgaagaag gaggagagcc
     5341 ttttcagaag aaacttcaaa gtggtgaacc agtggagtta gaaaaccccc ctctcctgcc
     5401 tgagtccact gtatcaccac aagcctcaac accaatatct cagagcacac cagtcttccc
     5461 tcctgggtca cttcctatcc catcccagcc tcagttttct catgacattt ttattccttc
     5521 cccaagtctg gaagaacaat caaatgatgg gaagaaagat ggagatatgc atagttcatc
     5581 tttgacagtt gagtgttcta aaacttcaga gattgaacca aagaattccc ctgaggatct
     5641 tgggctatct ttgacagggg attcttgcaa gttgatgctt tctacaagtg aatatagtca
     5701 gtccccaaag atggagagct tgagttctca cagaattgat gaagatggag aaaacacaca
     5761 gattgaggat acggaaccca tgtctccagt tctcaattct aaatttgttc ctgctgaaaa
     5821 tgatagtatc ctgatgaatc cagcacagga tggtgaagta caactgagtc agaatgatga
     5881 caaaacaaag ggagatgata cagacaccag ggatgacatt agtattttag ccactggttg
     5941 caagggcaga gaagaaacgg tagcagaaga tgtttgtatt gatctcactt gtgattcggg
     6001 gagtcaggca gttccgtcac cagctactcg atctgaggca ctttctagtg tgttagatca
     6061 ggaggaagct atggaaatta aagaacacca tccagaggag gggtcttcag ggtctgaggt
     6121 ggaagaaatc cctgagacac cttgtgaaag tcaaggagag gaactcaaag aagaaaatat
     6181 ggagagtgtt ccgttgcacc tttctctgac tgaaactcag tcccaagggt tgtgtcttca
     6241 aaaggaaatg ccaaaaaaag aatgctcaga agctatggaa gttgaaacca gtgtgattag
     6301 tattgattcc cctcaaaagt tggcaatact tgaccaagaa ttggaacata aggaacagga
     6361 agcttgggaa gaagctactt cagaggactc cagtgttgtc attgtagatg tgaaagagcc
     6421 atctcccaga gttgatgttt cttgtgaacc tttggaggga gtggagaagt gctcagattc
     6481 ccagtcatgg gaggatattg ctccagaaat agaaccatgt gctgagaata gattagacac
     6541 caaggaagaa aagagtgtag aatatgaagg agatctgaaa tcagggactg cagaaacaga
     6601 acctgtagag caagattctt cacagccttc cttaccttta gtgagagcag atgatccttt
     6661 aagacttgac caggagttgc agcagcccca aactcaggag aaaacaagta attcattaac
     6721 agaagactca aaaatggcta atgcaaagca gctaagctca gatgcagagg cccagaagct
     6781 ggggaagccc tctgcccatg cctcacaaag cttctgtgaa agttctagtg aaaccccatt
     6841 tcatttcact ttgcctaaag aaggtgatat catcccacca ttgactggtg caaccccacc
     6901 tcttattggg cacctaaaat tggagcccaa gagacacagt actcctattg gtattagcaa
     6961 ctatccagaa agcaccatag caaccagtga tgtcatgtct gaaagcatgg tggagaccca
     7021 tgatcccata cttgggagtg gaaaagggga ttctggggct gccccagacg tggatgataa
     7081 attatgtcta agaatgaaac tggttagtcc tgagactgag gcgagtgaag agtctttgca
     7141 gttcaacctg gaaaagcctg caactggtga aagaaaaaat ggatctactg ctgttgctga
     7201 gtctgttgcc agtccccaga agaccatgtc tgtgttgagc tgtatctgtg aagccaggca
     7261 agagaatgag gctcgaagtg aggatccccc caccacaccc atcaggggga acttgctcca
     7321 ctttccaagt tctcaaggag aagaggagaa agaaaaattg gagggtgacc atacaatcag
     7381 gcagagtcaa cagcctatga agcccattag tcctgtcaag gaccctgttt ctcctgcttc
     7441 ccagaagatg gtcatacaag ggccatccag tcctcaagga gaggcaatgg tgacagatgt
     7501 gctagaagac cagaaagaag gacggagtac taataaggaa aatcctagta aggccttgat
     7561 tgaaaggccc agccaaaata acataggaat ccaaaccatg gagtgttcct tgagggtccc
     7621 agaaactgtt tcagcagcaa cccagactat aaagaatgtg tgtgagcagg ggaccagtac
     7681 agtggaccag aactttggaa agcaagatgc cacagttcag actgagaggg ggagtggtga
     7741 gaaaccagtc agtgctcctg gggatgatac agagtcgctc catagccagg gagaagaaga
     7801 gtttgatatg cctcagcctc cacatggcca tgtcttacat cgtcacatga gaacaatccg
     7861 ggaagtacgc acacttgtca ctcgtgtcat tacagatgtg tattatgtgg atggaacaga
     7921 agtagaaaga aaagtaactg aggagactga agagccaatt gtagagtgtc aggagtgtga
     7981 aactgaagtt tccccttcac agactggggg ctcctcaggt gacctggggg atatcagctc
     8041 cttctcctcc aaggcatcca gcttacaccg cacatcaagt gggacaagtc tctcagctat
     8101 gcacagcagt ggaagctcag ggaaaggagc cggaccactc agagggaaaa ccagcgggac
     8161 agaacccgca gattttgcct tacccagctc ccgaggaggc ccaggaaaac tgagtcctag
     8221 aaaaggggtc agtcagacag ggacgccagt gtgtgaggag gatggtgatg caggccttgg
     8281 catcagacag ggagggaagg ctccagtcac gcctcgtggg cgtgggcgaa ggggccgccc
     8341 accttctcgg accactggaa ccagagaaac agctgtgcct ggccccttgg gcatagagga
     8401 catttcacct aacttgtcac cagatgataa atccttcagc cgtgtcgtgc cccgagtgcc
     8461 agactccacc agacgaacag atgtgggtgc tggtgctttg cgtcgtagtg actctccaga
     8521 aattcctttc caggctgctg ctggcccttc tgatggctta gatgcctcct ctccaggaaa
     8581 tagctttgta gggctccgtg ttgtagccaa gtggtcatcc aatggctact tttactctgg
     8641 gaaaatcaca cgagatgtcg gagctgggaa gtataaattg ctctttgatg atgggtacga
     8701 atgtgatgtg ttgggcaaag acattctgtt atgtgacccc atcccgctgg acactgaagt
     8761 gacggccctc tcggaggatg agtatttcag tgcaggagtg gtgaaaggac ataggaagga
     8821 gtctggggaa ctgtactaca gcattgaaaa agaaggccaa agaaagtggt ataagcgaat
     8881 ggctgtcatc ctgtccttgg agcaaggaaa cagactgaga gagcagtatg ggcttggccc
     8941 ctatgaagca gtaacacctc ttacaaaggc agcagatatc agcttagaca atttggtgga
     9001 agggaagcgg aaacggcgca gtaacgtcag ctccccagcc acccctactg cctccagtag
     9061 cagcagcaca acccctaccc gaaagatcac agaaagtcct cgtgcctcca tgggagttct
     9121 ctcaggcaaa agaaaactta tcacttctga agaggaacgg tcccctgcca agcgaggtcg
     9181 caagtctgcc acagtaaaac ctgtaggggc aggagagttt gtgagcccct gtgagagtgg
     9241 agacaacacc ggtgaaccct ctgccctgga agagcagaga gggcctttgc ctctcaacaa
     9301 gaccttgttt ctgggctacg catttctcct taccatggcc acaaccagtg acaagttggc
     9361 cagccgctcc aaactgccag atggtcctac aggaagcagt gaagaagagg aggaattttt
     9421 ggaaattcct cctttcaaca agcagtatac agaatcccag cttcgagcag gagctggcta
     9481 tatccttgaa gatttcaatg aagcccagtg taacacagct taccagtgtc ttctaattgc
     9541 ggatcagcat tgtcgaaccc ggaagtactt cctgtgcctt gccagtggga ttccttgtgt
     9601 gtctcatgtc tgggtccatg atagttgcca tgccaaccag ctccagaact accgtaatta
     9661 tctgttgcca gctgggtaca gccttgagga gcaaagaatt ctggactggc aaccccgtga
     9721 aaatcctttc cagaatctga aggtactctt ggtatcagac caacagcaga acttcctgga
     9781 gctctggtct gagatcctca tgactggtgg tgcagcctct gtgaagcagc accattcaag
     9841 tgcccataac aaagatattg ctttaggggt atttgatgtg gtggtgacgg acccctcatg
     9901 cccagcctcg gtgctgaagt gtgctgaagc attgcagctg cctgtggtgt cacaagagtg
     9961 ggtgatccag tgcctcattg ttggggagag aattggattc aagcagcatc caaaatataa
    10021 acacgattat gtttctcact agtaatgaac tagtccgtaa cttgaaagta tttcgatttc
    10081 ttggctttat atatcttgtg gaaaggacga tggacatcgg gcggacggtt gcacacgcgt
    10141 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
    10201 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
    10261 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
    10321 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
    10381 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
    10441 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
    10501 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
    10561 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
    10621 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
    10681 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
    10741 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
    10801 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
    10861 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
    10921 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
    10981 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
    11041 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
    11101 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
    11161 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    11221 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
    11281 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
    11341 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
    11401 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
    11461 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
    11521 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
    11581 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
    11641 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
    11701 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
    11761 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
    11821 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
    11881 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
    11941 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
    12001 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
    12061 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
    12121 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
    12181 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
    12241 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
    12301 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
    12361 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
    12421 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
    12481 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
    12541 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
    12601 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
    12661 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
    12721 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
    12781 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
    12841 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
    12901 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
    12961 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
    13021 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
    13081 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    13141 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    13201 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    13261 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    13321 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    13381 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    13441 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    13501 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    13561 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    13621 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    13681 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    13741 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    13801 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    13861 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    13921 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    13981 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
    14041 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    14101 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    14161 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    14221 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
    14281 tggagctgca agc
//
