LOCUS       sequence-471163-       15610 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15610)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 15610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2509
FEATURES             Location/Qualifiers
     source          1..15610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7256..9106
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      8870..8879
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    11467..12055
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11520..11540)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11938..11949)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12127..12360
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12438..12559
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12475..12494)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12529..12548
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12599..12734
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12754..12773)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12755..12773)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12783..12800)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12783..12799)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12792..12814)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12941..13396
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13028..13047)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13238..13259
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13422..13526
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13527..13595,13596..14387)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13745..13764)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14558..15146
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15047..15066
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15300..15317
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    15434..15455
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15470..15475,15476..15493,15494..15500)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15508..15524
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15513..15535
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15532..15548
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15532..15548
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     15567..15587
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        15569..15587
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcc gagaatgtgg tggaaccggg
     4141 gccgccttca gccaagcggc ctaaactctc atctccggcc ctctcggcgt ccgccagcga
     4201 tggcacagat tttggctctc tatttgactt ggagcacgac ttaccagatg aattaatcaa
     4261 ctctacagaa ttgggactaa ccaatggtgg tgatattaat cagcttcaga caagtcttgg
     4321 catggtacaa gatgcagctt ctaaacataa acagctgtca gaattgctgc gatctggtag
     4381 ttcccctaac ctcaatatgg gagttggtgg cccaggtcaa gtcatggcca gccaggccca
     4441 acagagcagt cctggattag gtttgataaa tagcatggtc aaaagcccaa tgacacaggc
     4501 aggcttgact tctcccaaca tggggatggg cactagtgga ccaaatcagg gtcctacgca
     4561 gtcaacaggt atgatgaaca gtccagtaaa tcagcctgcc atgggaatga acacagggat
     4621 gaatgcgggc atgaatcctg gaatgttggc tgcaggcaat ggacaaggga taatgcctaa
     4681 tcaagtcatg aacggttcaa ttggagcagg ccgagggcga cagaatatgc agtacccaaa
     4741 cccaggcatg ggaagtgctg gcaacttact gactgagcct cttcagcagg gctctcccca
     4801 gatgggagga caaacaggat tgagaggccc ccagcctctt aagatgggaa tgatgaacaa
     4861 ccccaatcct tatggttcac catatactca gaatcctgga cagcagattg gagccagtgg
     4921 ccttggtctc cagattcaga caaaaactgt actatcaaat aacttatctc catttgctat
     4981 ggacaaaaag gcagttcctg gtggaggaat gcccaacatg ggtcaacagc cagccccgca
     5041 ggtccagcag ccaggcctgg tgactccagt tgcccaaggg atgggttctg gagcacatac
     5101 agctgatcca gagaagcgca agctcatcca gcagcagctt gttctccttt tgcatgctca
     5161 caagtgccag cgccgggaac aggccaatgg ggaagtgagg cagtgcaacc ttccccactg
     5221 tcgcacaatg aagaatgtcc taaaccacat gacacactgc cagtcaggca agtcttgcca
     5281 agtggcacac tgtgcatctt ctcgacaaat catttcacac tggaagaatt gtacaagaca
     5341 tgattgtcct gtgtgtctcc ccctcaaaaa tgctggtgat aagagaaatc aacagccaat
     5401 tttgactgga gcacccgttg gacttggaaa tcctagctct ctaggggtgg gtcaacagtc
     5461 tgcccccaac ctaagcactg ttagtcagat tgatcccagc tccatagaaa gagcctatgc
     5521 agctcttgga ctaccctatc aagtaaatca gatgccgaca caaccccagg tgcaagcaaa
     5581 gaaccagcag aatcagcagc ctgggcagtc tccccaaggc atgcggccca tgagcaacat
     5641 gagtgctagt cctatgggag taaatggagg tgtaggagtt caaacgccga gtcttctttc
     5701 tgactcaatg ttgcattcag ccataaattc tcaaaaccca atgatgagtg aaaatgccag
     5761 tgtgccctcc ctgggtccta tgccaacagc agctcaacca tccactactg gaattcggaa
     5821 acagtggcac gaagatatta ctcaggatct tcgaaatcat cttgttcaca aactcgtcca
     5881 agccatattt cctacgccgg atcctgctgc tttaaaagac agacggatgg aaaacctagt
     5941 tgcatatgct cggaaagttg aaggggacat gtatgaatct gcaaacaatc gagcggaata
     6001 ctaccacctt ctagctgaga aaatctataa gatccagaaa gaactagaag aaaaacgaag
     6061 gaccagacta cagaagcaga acatgctacc aaatgctgca ggcatggttc cagtttccat
     6121 gaatccaggg cctaacatgg gacagccgca accaggaatg acttctaatg gccctctacc
     6181 tgacccaagt atgatccgtg gcagtgtgcc aaaccagatg atgcctcgaa taactccaca
     6241 atctggtttg aatcaatttg gccagatgag catggcccag ccccctattg taccccggca
     6301 aacccctcct cttcagcacc atggacagtt ggctcaacct ggagctctca acccgcctat
     6361 gggctatggg cctcgtatgc aacagccttc caaccagggc cagttccttc ctcagactca
     6421 gttcccatca cagggaatga atgtaacaaa tatccctttg gctccgtcca gcggtcaagc
     6481 tccagtgtct caagcacaaa tgtctagttc ttcctgcccg gtgaactctc ctataatgcc
     6541 tccagggtct caggggagcc acattcactg tccccagctt cctcaaccag ctcttcatca
     6601 gaattcaccc tcgcctgtac ctagtcgtac ccccacccct caccatactc ccccaagcat
     6661 aggggctcag cagccaccag caacaacaat tccagcccct gttcctacac ctcctgccat
     6721 gccacctggg ccacagtccc aggctctaca tccccctcca aggcagacac ctacaccacc
     6781 aacaacacaa cttccccaac aagtgcagcc ttcacttcct gctgcacctt ctgctgacca
     6841 gccccagcag cagcctcgct cacagcagag cacagcagcg tctgttccta ccccaacagc
     6901 accgctgctt cctccgcagc ctgcaactcc actttcccag ccagctgtaa gcattgaagg
     6961 acaggtatca aatcctccat ctactagtag cacagaagtg aattctcagg ccattgctga
     7021 gaagcagcct tcccaggaag tgaagatgga ggccaaaatg gaagtggatc aaccagaacc
     7081 agcagatact cagccggagg atatttcaga gtctaaagtg gaagactgta aaatggaatc
     7141 taccgaaaca gaagagagaa gcactgagtt aaaaactgaa ataaaagagg aggaagacca
     7201 gccaagtact tcagctaccc agtcatctcc ggctccagga cagtcaaaga aaaagatttt
     7261 caaaccagaa gaactacgac aggcactgat gccaactttg gaggcacttt accgtcagga
     7321 tccagaatcc cttccctttc gtcaacctgt ggaccctcag cttttaggaa tccctgatta
     7381 ctttgatatt gtgaagagcc ccatggatct ttctaccatt aagaggaagt tagacactgg
     7441 acagtatcag gagccctggc agtatgtcga tgatatttgg cttatgttca ataatgcctg
     7501 gttatataac cggaaaacat cacgggtata caaatactgc tccaagctct ctgaggtctt
     7561 tgaacaagaa attgacccag tgatgcaaag ccttggatac tgttgtggca gaaagttgga
     7621 gttctctcca cagacactgt gttgctacgg caaacagttg tgcacaatac ctcgtgatgc
     7681 cacttattac agttaccaga acaggtatca tttctgtgag aagtgtttca atgagatcca
     7741 aggggagagc gtttctttgg gggatgaccc ttcccagcct caaactacaa taaataaaga
     7801 acaattttcc aagagaaaaa atgacacact ggatcctgaa ctgtttgttg aatgtacaga
     7861 gtgcggaaga aagatgcatc agatctgtgt ccttcaccat gagatcatct ggcctgctgg
     7921 attcgtctgt gatggctgtt taaagaaaag tgcacgaact aggaaagaaa ataagttttc
     7981 tgctaaaagg ttgccatcta ccagacttgg cacctttcta gagaatcgtg tgaatgactt
     8041 tctgaggcga cagaatcacc ctgagtcagg agaggtcact gttagagtag ttcatgcttc
     8101 tgacaaaacc gtggaagtaa aaccaggcat gaaagcaagg tttgtggaca gtggagagat
     8161 ggcagaatcc tttccatacc gaaccaaagc cctctttgcc tttgaagaaa ttgatggtgt
     8221 tgacctgtgc ttctttggca tgcatgttca agagtatggc tctgactgcc ctccacccaa
     8281 ccagaggaga gtatacatat cttacctcga tagtgttcat ttcttccgtc ctaaatgctt
     8341 gaggactgca gtctatcatg aaatcctaat tggatattta gaatatgtca agaaattagg
     8401 ttacacaaca gggcatattt gggcatgtcc accaagtgag ggagatgatt atatcttcca
     8461 ttgccatcct cctgaccaga agatacccaa gcccaagcga ctgcaggaat ggtacaaaaa
     8521 aatgcttgac aaggctgtat cagagcgtat tgtccatgac tacaaggata tttttaaaca
     8581 agctactgaa gatagattaa caagtgcaaa ggaattgcct tatttcgagg gtgatttctg
     8641 gcccaatgtt ctggaagaaa gcattaagga actggaacag gaggaagaag agagaaaacg
     8701 agaggaaaac accagcaatg aaagcacaga tgtgaccaag ggagacagca aaaatgctaa
     8761 aaagaagaat aataagaaaa ccagcaaaaa taagagcagc ctgagtaggg gcaacaagaa
     8821 gaaacccggg atgcccaatg tatctaacga cctctcacag aaactatatg ccaccatgga
     8881 gaagcataaa gaggtcttct ttgtgatccg cctcattgct ggccctgctg ccaactccct
     8941 gcctcccatt gttgatcctg atcctctcat cccctgcgat ctgatggatg gtcgggatgc
     9001 gtttctcacg ctggcaaggg acaagcacct ggagttctct tcactccgaa gagcccagtg
     9061 gtccaccatg tgcatgctgg tggagctgca cacgcagagc caggaccgct ttgtctacac
     9121 ctgcaatgaa tgcaagcacc atgtggagac acgctggcac tgtactgtct gtgaggatta
     9181 tgacttgtgt atcacctgct ataacactaa aaaccatgac cacaaaatgg agaaactagg
     9241 ccttggctta gatgatgaga gcaacaacca gcaggctgca gccacccaga gcccaggcga
     9301 ttctcgccgc ctgagtatcc agcgctgcat ccagtctctg gtccatgctt gccagtgtcg
     9361 gaatgccaat tgctcactgc catcctgcca gaagatgaag cgggttgtgc agcataccaa
     9421 gggttgcaaa cggaaaacca atggcgggtg ccccatctgc aagcagctca ttgccctctg
     9481 ctgctaccat gccaagcact gccaggagaa caaatgcccg gtgccgttct gcctaaacat
     9541 caagcagaag ctccggcagc aacagctgca gcaccgacta cagcaggccc aaatgcttcg
     9601 caggaggatg gccagcatgc agcggactgg tgtggttggg cagcaacagg gcctcccttc
     9661 ccccactcct gccactccaa cgacaccaac tggccaacag ccaaccaccc cgcagacgcc
     9721 ccagcccact tctcagcctc agcctacccc tcccaatagc atgccaccct acttgcccag
     9781 gactcaagct gctggccctg tgtcccaggg taaggcagca ggccaggtga cccctccaac
     9841 ccctcctcag actgctcagc caccccttcc agggccccca cctgcagcag tggaaatggc
     9901 aatgcagatt cagagagcag cggagacgca gcgccagatg gcccacgtgc aaatttttca
     9961 aaggccaatc caacaccaga tgcccccgat gactcccatg gcccccatgg gtatgaaccc
    10021 acctcccatg accagaggtc ccagtgggca tttggagcca gggatgggac cgacagggat
    10081 gcagcaacag ccaccctgga gccaaggagg attgcctcag ccccagcaac tacagtctgg
    10141 gatgccaagg ccagccatga tgtcagtggc ccagcatggt caacctttga acatggctcc
    10201 acaaccagga ttgggccagg taggtatcag cccactcaaa ccaggcactg tgtctcaaca
    10261 agccttacaa aaccttttgc ggactctcag gtctcccagc tctcccctgc agcagcaaca
    10321 ggtgcttagt atccttcacg ccaaccccca gctgttggct gcattcatca agcagcgggc
    10381 tgccaagtat gccaactcta atccacaacc catccctggg cagcctggca tgccccaggg
    10441 gcagccaggg ctacagccac ctaccatgcc aggtcagcag ggggtccact ccaatccagc
    10501 catgcagaac atgaatccaa tgcaggcggg cgttcagagg gctggcctgc cccagcagca
    10561 accacagcag caactccagc cacccatggg agggatgagc ccccaggctc agcagatgaa
    10621 catgaaccac aacaccatgc cttcacaatt ccgagacatc ttgagacgac agcaaatgat
    10681 gcaacagcag cagcaacagg gagcagggcc aggaataggc cctggaatgg ccaaccataa
    10741 ccagttccag caaccccaag gagttggcta cccaccacag cagcagcagc ggatgcagca
    10801 tcacatgcaa cagatgcaac aaggaaatat gggacagata ggccagcttc cccaggcctt
    10861 gggagcagag gcaggtgcca gtctacaggc ctatcagcag cgactccttc agcaacagat
    10921 ggggtcccct gttcagccca accccatgag cccccagcag catatgctcc caaatcaggc
    10981 ccagtcccca cacctacaag gccagcagat ccctaattct ctctccaatc aagtgcgctc
    11041 tccccagcct gtcccttctc cacggccaca gtcccagccc ccccactcca gtccttcccc
    11101 aaggatgcag cctcagcctt ctccacacca cgtttcccca cagacaagtt ccccacatcc
    11161 tggactggta gctgcccagg ccaaccccat ggaacaaggg cattttgcca gcccggacca
    11221 gaattcaatg ctttctcagc ttgctagcaa tccaggcatg gcaaacctcc atggtgcaag
    11281 cgccacggac ctgggactca gcaccgataa ctcagacttg aattcaaacc tctcacagag
    11341 tacactagac atacactagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
    11401 gctttatata tcttgtggaa aggacgagat gtcaagatgg cccgatagcg cacgcgttaa
    11461 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
    11521 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
    11581 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
    11641 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
    11701 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
    11761 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
    11821 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
    11881 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
    11941 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
    12001 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
    12061 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
    12121 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
    12181 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
    12241 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
    12301 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
    12361 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
    12421 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    12481 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    12541 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
    12601 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
    12661 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
    12721 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
    12781 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
    12841 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
    12901 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
    12961 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
    13021 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
    13081 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
    13141 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
    13201 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
    13261 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    13321 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    13381 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    13441 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    13501 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    13561 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    13621 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    13681 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    13741 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    13801 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    13861 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    13921 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    13981 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    14041 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    14101 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    14161 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    14221 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    14281 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    14341 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    14401 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    14461 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    14521 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    14581 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    14641 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    14701 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    14761 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    14821 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    14881 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    14941 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    15001 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    15061 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    15121 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    15181 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    15241 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    15301 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    15361 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    15421 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    15481 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    15541 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
    15601 agctgcaagc
//
