LOCUS       sequence-471168-       10288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10288)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3062
FEATURES             Location/Qualifiers
     source          1..10288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6145..6733
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6198..6218)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6616..6627)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6805..7038
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7116..7237
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7153..7172)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7207..7226
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7277..7412
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7432..7451)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7433..7451)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7461..7478)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7461..7477)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7470..7492)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7619..8074
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7706..7725)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7916..7937
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8100..8204
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8205..8273,8274..9065)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8423..8442)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9236..9824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9725..9744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9978..9995
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10112..10133
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10148..10153,10154..10171,10172..10178)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10186..10202
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10191..10213
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10210..10226
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10210..10226
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10245..10265
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10247..10265
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcc gaggcagcgc tagtgattac
     4141 gccgcagatt cccatggtaa cagaagagtt tgtgaaacca tcacagggcc atgtgacctt
     4201 tgaggatatt gctgtgtact tctcccagga ggagtggggc ctccttgatg aagctcaaag
     4261 gtgcctgtat catgatgtga tgctggagaa cttttcgctt atggcctcag taggttgttt
     4321 gcatggaata gaggctgagg aggccccttc tgagcagact ctttctgcgc aaggagtgtc
     4381 acaggccagg actccaaagc taggtccttc catcccaaat gctcattctt gtgagatgtg
     4441 tatcctggtc atgaaagaca ttttgtacct cagtgagcat caggggacac ttccctggca
     4501 gaaaccttat acgtctgtgg ccagtgggaa atggttttca tttggttcta acctgcaaca
     4561 gcaccagaac caggacagtg gagagaaaca catcagaaag gaggagagca gtgccttgct
     4621 tctgaatagc tgcaaaattc ctctgtcaga caatcttttc ccatgcaaag atgttgagaa
     4681 ggattttcca accatcctgg gccttctcca acaccagacc acccacagca gacaagagta
     4741 tgcacataga agcagggaga cctttcaaca aagacgttac aaatgtgagc aagttttcaa
     4801 tgagaaagtt catgttactg agcatcagag agtccacact ggagaaaaag cttataagcg
     4861 tagggaatat gggaaatcct tgaactctaa atacttattt gttgaacacc agagaaccca
     4921 taatgcagaa aagccttatg tgtgcaatat atgtgggaaa tcattcctcc ataaacaaac
     4981 actcgttggg caccagcaga gaattcacac tagagaaagg tcttatgtgt gcatcgaatg
     5041 tgggaaatcc ttgagctcca aatactcact tgtggaacac cagagaaccc ataatggaga
     5101 aaagccttat gtgtgcaatg tatgtgggaa atcattccgc cacaaacaaa catttgttgg
     5161 ccatcagcag agaatccaca ctggagagag gccttatgtg tgtatggaat gtgggaaatc
     5221 ttttattcat tcctatgacc gcattcgaca ccagagagtt cacactggag aaggggctta
     5281 tcagtgcagt gaatgtggga aatccttcat atacaaacag tcacttcttg atcaccatag
     5341 aatccacacg ggagaaaggc cttatgagtg caaagaatgt gggaaggcct tcattcacaa
     5401 aaaaagactt cttgagcacc agagaattca tactggagaa aagccttatg tgtgcatcat
     5461 atgtgggaaa tcatttatcc gctcgtctga ctacatgcga caccagagaa ttcacactgg
     5521 agaaagggct tatgaatgca gtgactgtgg gaaagccttc atctccaaac aaacacttct
     5581 taagcatcac aaaatccaca ctagagaaag gccttatgaa tgcagtgaat gtggaaaagg
     5641 cttctacctt gaggttaaac ttcttcagca ccaaagaatc catactagag aacaactttg
     5701 tgagtgcaat gaatgtggaa aagtcttcag ccaccaaaaa agacttcttg agcaccagaa
     5761 agttcacact ggcgaaaagc cctgtgagtg cagtgaatgt gggaaatgct ttagacaccg
     5821 caccagcctc attcaacacc agaaagttca cagtggagag aggccttata actgcactgc
     5881 atgtgagaag gcctttatct ataaaaacaa acttgttgag catcagcgaa tccacaccgg
     5941 agaaaagccg tatgaatgtg gtaaatgtgg gaaagccttc aacaaaagat attcccttgt
     6001 caggcaccag aaggtacata taacagaaga gccctagtaa tgaactagtc cgtaacttga
     6061 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgacctgt ctgagccgcc
     6121 cccctcttga cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     6181 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6241 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6301 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     6361 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     6421 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     6481 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     6541 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     6601 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     6661 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     6721 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6781 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     6841 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6901 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6961 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7021 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     7081 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     7141 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7201 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     7261 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7321 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     7381 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     7441 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     7501 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     7561 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     7621 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     7681 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     7741 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     7801 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     7861 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     7921 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     7981 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     8041 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     8101 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     8161 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     8221 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     8281 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     8341 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     8401 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     8461 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     8521 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     8581 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     8641 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     8701 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     8761 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     8821 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     8881 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     8941 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     9001 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     9061 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     9121 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     9181 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     9241 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     9301 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     9361 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     9421 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     9481 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     9541 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     9601 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     9661 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     9721 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     9781 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     9841 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     9901 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     9961 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    10021 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    10081 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    10141 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    10201 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
    10261 aaagggaaca aaagctggag ctgcaagc
//
