LOCUS       sequence-471183-        9770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HA-tagged NUP98::PRRX1 in dual promoter lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9770)
  AUTHORS   Michmerhuizen NL, Heikamp EB, Iacobucci I, Umeda M, Arthur B, Mishra
            V, Park CS, Di Giacomo D, Hiltenbrand R, Gao Q, Radko-Juettner S, 
            Lott J, Martucci C, Subramanyam V, Hatton C, Wenge DV, Baviskar P, 
            Portola P, Claquin A, Chandra B, Baggett DW, Khalighifar A, Huang H,
            Zhou P, Long L, Shi H, Sun Y, Papachristou EK, Sekhar Reddy 
            Chilamakuri C, de Luna Vitorino FN, Gongora JM, Wu H, Pounds SB, 
            Janke LJ, Kentsis A, D'Santos CS, Garcia BA, Kriwacki RW, Chi H, 
            Klco JM, Armstrong SA, Mullighan CG
  TITLE     KAT6A and KAT7 Histone Acetyltransferase Complexes Are Molecular 
            Dependencies and Therapeutic Targets in NUP98-Rearranged Acute 
            Myeloid Leukemia.
  JOURNAL   Cancer Discov. 2025 Jun 19. doi: 10.1158/2159-8290.CD-24-1772.
  PUBMED    40536430
REFERENCE   2  (bases 1 to 9770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2025 Jun 19. doi: 10.1158/2159-8290.CD-24-1772."
COMMENT     Sequence Label: CCLMPC_HA-NUP98::PRRX1
FEATURES             Location/Qualifiers
     source          1..9770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        1131..1510
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1511..1714
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1665..1685
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1729..1909
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1956..2081
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2574..2807
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2992..3036
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3333..3450
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4053..4077
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4101..4127
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(5935..5959)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5988..6487
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6023..6046)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6420..6439
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      6508..6517
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6514..7224
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6659..6677)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6927..6946
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             7346..7579
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7651..7772
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7688..7707)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7742..7761
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7812..7947
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7967..7986)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7968..7986)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7996..8013)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7996..8012)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8005..8027)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8154..8609
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8241..8260)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8451..8472
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8635..8739
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8740..8808,8809..9600)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8958..8977)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttggcca ttgcatacgt tgtatccata
     1081 tcataatatg tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt
     1141 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1201 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1261 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1321 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1381 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1441 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1501 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     1561 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     1621 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     1681 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccggg gtctctctgg
     1741 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     1801 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     1861 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     1921 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     1981 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     2041 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     2101 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     2161 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     2221 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     2281 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     2341 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     2401 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     2461 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     2521 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     2581 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     2641 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     2701 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     2761 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     2821 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     2881 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2941 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     3001 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     3061 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     3121 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     3181 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     3241 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgataa
     3301 gctaattcac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     3361 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     3421 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     3481 gtttgggaat tagcttgatc gattagtcca atttgttaaa gacaggatat cagtggtcca
     3541 ggctctagtt ttgactcaac aatatcacca gctgaagcct atagagtacg agccatagat
     3601 agaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag
     3661 gtttggcaag ctaggatcaa ggttaggaac agagagacag cagaatatgg gccaaacagg
     3721 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagttgg aacagcagaa
     3781 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     3841 gatggtcccc agatgcggtc ccgccctcag cagtttctag agaaccatca gatgtttcca
     3901 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
     3961 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
     4021 actcggcgcg atctagatct cgaatcgaat tcacaagttt gtacaaaaaa gcaggctccg
     4081 cggccgcccc cttcaccatg tacccatacg atgttccaga ttacgctttt aacaaatcat
     4141 ttggaacacc ctttgggggt ggcacaggtg gctttggcac aacttcaaca tttggacaga
     4201 atactggctt tggcactact agtggagggg catttggaac atctgcattt ggttctagca
     4261 acaatactgg aggcctcttt ggaaattcac agactaaacc aggaggattg tttggaacca
     4321 gttcatttag ccagccagct acctccacaa gcactggctt tgggtttggt acgtcaacag
     4381 gaacagcaaa taccttgttt ggaactgcaa gcacagggac cagtctcttc tcatcccaaa
     4441 acaatgcctt tgcacaaaat aaaccaactg gctttggcaa ttttggaacc agtactagca
     4501 gtggaggact ctttggaacc acaaatacca cctctaatcc ttttggcagc acatctggct
     4561 ccctctttgg gccaagtagt tttacagctg ctcctactgg gactactatt aaatttaacc
     4621 ctccaactgg tacagatact atggtcaaag ctggagttag cactaacata agtaccaagc
     4681 accagtgtat tactgctatg aaagaatatg aaagcaagtc actagaggaa cttcgtttag
     4741 aggattatca ggctaacagg aagggcccac agaaccaggt gggagcaggt accacaactg
     4801 gcttgtttgg gtcttctcca gccacttcca gcgcaacagg actcttcagc tcctccacca
     4861 ctaattcagg ctttgcatat ggtacaactg gatttggaac aaatccaggt ggtctctttg
     4921 gccaacagaa tcagcagact accagcctct tcagcaaacc atttggccag gctacaacca
     4981 cccagaacac tggcttttcc tttggtaata ccagcaccat aggacagcca agcaccaaca
     5041 ccatgggatt atttggagta acccaagcct cacagcctgg aggtcttttt gggacagcta
     5101 caaacaccag cactgggaca gcatttggaa caggaacagg tctctttggg cagaccaata
     5161 ctggatttgg tgctgttggt tcgaccctgt ttggcaataa caagcttact acatttggaa
     5221 gcagcacaac cagtgcacct tcatttggta caaccaatgg cgggctcttt ggttttggca
     5281 caaataccag tgggaatagt atttttggaa gtaaaccagc acctgggact cttggaactg
     5341 ggcttggtgc aggatttgga acagctcttg gtgctggaca ggcatctttg tctgggaaca
     5401 accaacctaa gattggaggg cctcttggta caggagcctt tggggcccct ggatttaata
     5461 ctacgacagc cactttgggc tttggagccc cccaggcccc agtagatgac cagctgaact
     5521 cagaagaaaa aaagaagaga aagcagcgaa ggaataggac aaccttcaat agcagccagc
     5581 tgcaggcttt ggagcgtgtc tttgagcgga cacactatcc tgatgctttt gtgcgagaag
     5641 accttgcccg ccgggtgaac ctcaccgagg cgagagtgca ggtgtggttt cagaaccgaa
     5701 gagccaagtt ccgcaggaat gagagagcca tgctagccaa taaaaacgct tccctcctca
     5761 aatcctactc aggagacgtg actgctgtgg agcagcccat cgtacctcgt cctgctccga
     5821 gacccaccga ttatctctcc tgggggacag cgtctccgta cagatcctcg tccctcccaa
     5881 gatgttgttt acacgagggg cttcataacg gattctaaaa gggtgggcgc gccgacccag
     5941 ctttcttgta caaagtggtg atatcggtac cgagctcgaa ttctaccggg taggggaggc
     6001 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     6061 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     6121 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     6181 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     6241 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     6301 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     6361 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     6421 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     6481 cctttcgacc atctagatcc accgcgggcc accatggtga gcaagggcga ggaggataac
     6541 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     6601 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     6661 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     6721 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     6781 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     6841 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     6901 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     6961 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     7021 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     7081 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     7141 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     7201 ggcatggacg agctgtacaa gtagagcggc caactcgagg gatcccccgg ggtcgactga
     7261 tcaaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     7321 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     7381 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7441 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7501 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7561 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     7621 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     7681 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7741 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     7801 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7861 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7921 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     7981 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     8041 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     8101 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     8161 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     8221 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     8281 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     8341 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     8401 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     8461 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8521 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     8581 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     8641 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     8701 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     8761 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     8821 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     8881 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     8941 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     9001 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     9061 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     9121 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     9181 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     9241 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     9301 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     9361 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     9421 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     9481 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     9541 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     9601 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     9661 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     9721 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
