LOCUS       sequence-47119-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3010-07
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..757
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     CDS             47..757
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(74..102)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     697..717
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 acggatcccc gggcttaaga tcggtgacgg tgctggttta attaacagta aaggagaaga
       61 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
      121 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
      181 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttgacttg
      241 gggtgttcaa tgctttgcta gatacccaga tcatatgaaa cagcatgact ttttcaagag
      301 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta
      361 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa
      421 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaacgctat
      481 ttcagataat gtatacatca ctgcagacaa acaaaagaat ggaatcaaag ctaatttcaa
      541 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
      601 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
      661 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
      721 tgctgggatt acactaggca tggatgaact atacaaatag ggcgcgccag atctgtttag
      781 cttgcctcgt ccccgccggg tcacccggcc agcgacatgg aggcccaga
//
