LOCUS       sequence-471195-        3560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid mediates chloramphenicol resistance in the dark, 
            resulting in Cm mediated cell death upon illumination with blue 
            light.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Wolf B, Shehu P, Brenker L, von Bachmann A, Kroell A-S, Southern N, 
            Holderbach S, Eigenmann J, Aschenbrenner S, Mathony J, Niopek D
  TITLE     Rational engineering of allosteric protein switches by in silico 
            prediction of domain insertion sites
  JOURNAL   bioRxiv 2024.12.04.626757
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.12.04.626757"
COMMENT     Sequence Label: BLA_CAT_K136_GPG_LOV2
FEATURES             Location/Qualifiers
     source          1..3560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..121
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(188..207)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1243..1337
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(1357..1384)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1476..1562)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1715..1732
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     promoter        1780..1884
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1885..1953,1954..2745)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2103..2122)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2916..3504
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3405..3424
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgaacaat aaaactgttg atcggcacgt aagaggttcc aactttcacc ataatgaaat
       61 aagatcacta ccgggcgtat ttttttagtt atcgagattt tcaggagcta aggaagctaa
      121 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
      181 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
      241 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
      301 tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg
      361 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
      421 aacgttttca tcgctcttga gtgaatacca cgacgatttc cggcagtttc tacacatata
      481 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag gtccgggctt
      541 agccacaacg ctggaacgca ttgaaaagaa tttcgtaatc acagacccgc gccttcccga
      601 caatccaatt atttttgcgt ccgatagctt cctgcaatta accgaataca gccgcgaaga
      661 aattctgggt cgtaattgtc gcttccttca ggggccagag actgaccgtg ctacggtacg
      721 caaaatccgc gacgcaatcg acaatcaaac ggaagtcacg gttcagttga ttaactatac
      781 gaagagcgga aaaaaattct ggaatttatt tcacttgcag cctatgcgtg accagaaggg
      841 cgatgtccag tatttcattg gcgttcagct tgatggtacc gagcatgttc gcgatgctgc
      901 ggagcgtgaa ggtgtaatgt taattaaaaa gactgctgaa aacattgatg aggcggccaa
      961 aggtccgggc gggtttattg agaatatgtt tttcgtttca gccaatccct gggtgagttt
     1021 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     1081 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     1141 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     1201 tgagtggcag ggcggggcgt aatttgatat cgagctcgct tggactcctg ttgatagatc
     1261 cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt
     1321 tttttattgg tgagaatata tagttcctcc tttcagaaaa ggccatccgt caggatggcc
     1381 ttctgcttaa tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc
     1441 cgttgcttcg caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc
     1501 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
     1561 tgatgcctgg cagttcccta ctctcgcatg gggatacccc acactaccat cggcgctacg
     1621 gcgtttcact tctgagttcg gcatggggtc aggtgggacc accgcgctac tgccgccagg
     1681 caaattctgt tttatcagac cgcttctgcg ttctgattta atctgtatca ggctgaaaaa
     1741 tcgtcgacga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1801 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1861 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1921 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1981 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     2041 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     2101 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     2161 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     2221 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     2281 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     2341 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     2401 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     2461 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2521 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2581 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2641 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2701 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2761 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2821 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2881 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2941 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3001 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3061 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3121 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3181 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3241 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3301 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3361 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3421 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3481 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3541 ttttgctggc cttttgctca
//
