LOCUS       sequence-471199-       19369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TRV1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19369)
  AUTHORS   Weiss T, Kamalu M, Shi H, Li Z, Amerasekera J, Zhong Z, Adler BA, 
            Song MM, Vohra K, Wirnowski G, Chitkara S, Ambrose C, Steinmetz N, 
            Sridharan A, Sahagun D, Banfield JF, Doudna JA, Jacobsen SE
  TITLE     Viral delivery of an RNA-guided genome editor for transgene-free 
            germline editing in Arabidopsis.
  JOURNAL   Nat Plants. 2025 Apr 22. doi: 10.1038/s41477-025-01989-9.
  PUBMED    40263581
REFERENCE   2  (bases 1 to 19369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2025 Apr 22. doi: 10.1038/s41477-025-01989-9."
COMMENT     Sequence Label: pTW1169
FEATURES             Location/Qualifiers
     source          1..19369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        421..766
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      7620..7872
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(7881..7898)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7881..7897)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7890..7912)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8037..8056)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8247..8268
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8515..8539
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10135..11283)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKTYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11582..12376)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12568..13335
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      13958..14669
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(14796..15167)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            15200..15309
                     /note="incP origin of transfer"
     primer_bind     15532..15551
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15785..15802
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(15990..16640)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    17105..17129
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        17660..18005
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             18043..18594
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      18632..18832
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     protein_bind    19128..19149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(19164..19169,19170..19187,19188..19194)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    19202..19218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     19207..19229
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     19226..19242
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     19226..19242
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcatgcctgc aggtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa
       61 gatacagtct cagaagacca gagggctatt gagacttttc aacaaagggt aatatcggga
      121 aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag
      181 gaagatggct tctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc
      241 tctaccgaca gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa
      301 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atggtcaaca tggtggagca
      361 cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc agagggctat
      421 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
      481 ctgtcacttc atcgaaagga cagtagaaaa ggaagatggc ttctacaaat gccatcattg
      541 cgataaagga aaggctatcg ttcaagatgc ctctaccgac agtggtccca aagatggacc
      601 cccacccacg aggaacatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      661 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      721 agacccttcc tctatataag gaagttcatt tcatttggag aggataaaac atttcaatcc
      781 tttgaacgcg gtagaacgtg ctaattggat tttggtgaga acgcggtaga acgtacttat
      841 cacctacagt tttattttgt ttttcttttt ggtttaatct atccagctta gtaccgagtg
      901 ggggaaagtg actggtgtgc ctaaaacctt ttctttgata ctttgtaaaa atacatacag
      961 atacaatggc gaacggtaac ttcaagttgt ctcaattgct caatgtggac gagatgtctg
     1021 ctgagcagag gagtcatttc tttgacttga tgctgactaa acctgattgt gagatcgggc
     1081 aaatgatgca aagagttgtt gttgataaag tcgatgacat gattagagaa agaaagacta
     1141 aagatccagt gattgttcat gaagttcttt ctcagaagga acagaacaag ttgatggaaa
     1201 tttatcctga attcaatatc gtgtttaaag acgacaaaaa catggttcat gggtttgcgg
     1261 ctgctgagcg aaaactacaa gctttattgc ttttagatag agttcctgct ctgcaagagg
     1321 tggatgacat cggtggtcaa tggtcgtttt gggtaactag aggtgagaaa aggattcatt
     1381 cctgttgtcc aaatctagat attcgggatg atcagagaga aatttctcga cagatatttc
     1441 ttactgctat tggtgatcaa gctagaagtg gtaagagaca gatgtcggag aatgagctgt
     1501 ggatgtatga ccaatttcgt gaaaatattg ctgcgcctaa cgcggttagg tgcaataata
     1561 catatcaggg ttgtacatgt aggggttttt ctgatggtaa gaagaaaggc gcgcagtatg
     1621 cgatagctct tcacagcctg tatgacttca agttgaaaga cttgatggct actatggttg
     1681 agaagaaaac taaagtggtt catgctgcta tgctttttgc tcctgaaagt atgttagtgg
     1741 acgaaggtcc attaccttct gttgacggtt actacatgaa gaagaacggg aagatctatt
     1801 tcggttttga gaaagatcct tccttttctt acattcatga ctgggaagag tacaagaagt
     1861 atctactggg gaagccagtg agttaccaag ggaatgtgtt ctacttcgaa ccgtggcagg
     1921 tgagaggaga cacaatgctt ttttcgatct acaggatagc tggagttccg aggaggtctc
     1981 tatcatcgca agagtactac cgaagaatat atatcagtag atgggaaaac atggttgttg
     2041 tcccaatttt cgatctggtc gaatcaacgc gagagttggt caagaaagac ctgtttgtag
     2101 agaaacaatt catggacaag tgtttggatt acatagctag gttatctgac cagcagctga
     2161 ccataagcaa tgttaaatca tacttgagtt caaataattg ggtcttattc ataaacgggg
     2221 cggccgtgaa gaacaagcaa agtgtagatt ctcgagattt acagttgttg gctcaaactt
     2281 tgctagtgaa ggaacaagtg gcgagacctg tcatgaggga gttgcgtgaa gcaattctga
     2341 ctgagacgaa acctatcacg tcattgactg atgtgctggg tttaatatca agaaaactgt
     2401 ggaagcagtt tgctaacaag atcgcagtcg gcggattcgt tggcatggtt ggtactctaa
     2461 ttggattcta tccaaagaag gtactaacct gggcgaagga cacaccaaat ggtccagaac
     2521 tatgttacga gaactcgcac aaaaccaagg tgatagtatt tctgagtgtt gtgtatgcca
     2581 ttggaggaat cacgcttatg cgtcgagaca tccgagatgg actggtgaaa aaactatgtg
     2641 atatgtttga tatcaaacgg ggggcccatg tcttagacgt tgagaatccg tgccgctatt
     2701 atgaaatcaa cgatttcttt agcagtctgt attcggcatc tgagtccggt gagaccgttt
     2761 taccagattt atccgaggta aaagccaagt ctgataagct attgcagcag aagaaagaaa
     2821 tcgctgacga gtttctaagt gcaaaattct ctaactattc tggcagttcg gtgagaactt
     2881 ctccaccatc ggtggtcggt tcatctcgaa gcggactggg tctgttgttg gaagacagta
     2941 acgtgctgac ccaagctaga gttggagttt caagaaaggt agacgatgag gagatcatgg
     3001 agcagtttct gagtggtctt attgacactg aagcagaaat tgacgaggtt gtttcagcct
     3061 tttcagctga atgtgaaaga ggggaaacaa gcggtacaaa ggtgttgtgt aaacctttaa
     3121 cgccaccagg atttgagaac gtgttgccag ctgtcaaacc tttggtcagc aaaggaaaaa
     3181 cggtcaaacg tgtcgattac ttccaagtga tgggaggtga gagattacca aaaaggccgg
     3241 ttgtcagtgg agacgattct gtggacgcta gaagagagtt tctgtactac ttagatgcgg
     3301 agagagtcgc tcaaaatgat gaaattatgt ctctgtatcg tgactattcg agaggagtta
     3361 ttcgaactgg aggtcagaat tacccgcacg gactgggagt gtgggatgtg gagatgaaga
     3421 actggtgcat acgtccagtg gtcactgaac atgcttatgt gttccaacca gacaaacgta
     3481 tggatgattg gtcgggatac ttagaagtgg ctgtttggga acgaggtatg ttggtcaacg
     3541 acttcgcggt cgaaaggatg agtgattatg tcatagtttg cgatcagacg tatctttgca
     3601 ataacaggtt gatcttggac aatttaagtg ccctggatct aggaccagtt aactgttctt
     3661 ttgaattagt tgacggtgta cctggttgtg gtaagtcgac aatgattgtc aactcagcta
     3721 atccttgtgt cgatgtggtt ctctctactg ggagagcagc aaccgacgac ttgatcgaga
     3781 gattcgcgag caaaggtttt ccatgcaaat tgaaaaggag agtgaagacg gttgattctt
     3841 ttttgatgca ttgtgttgat ggttctttaa ccggagacgt gttgcatttc gatgaagctc
     3901 tcatggccca tgctggtatg gtgtactttt gcgctcagat agctggtgct aaacgatgta
     3961 tctgtcaagg agatcagaat caaatttctt tcaagcctag ggtatctcaa gttgatttga
     4021 ggttttctag tctggtcgga aagtttgaca ttgttacaga aaaaagagaa acttacagaa
     4081 gtccagcaga tgtggctgcc gtattgaaca agtactatac tggagatgtc agaacacata
     4141 acgcgactgc taattcgatg acggtgagga agattgtgtc taaagaacag gtttctttga
     4201 agcctggtgc tcagtacata actttccttc agtctgagaa gaaggagttg gtaaatttgt
     4261 tggcattgag gaaagtggca gctaaagtga gtacagtaca cgagtcgcaa ggagagacat
     4321 tcaaagatgt agtcctagtc aggacgaaac ctacggatga ctcaatcgct agaggtcggg
     4381 agtacttaat cgtggcgttg tcgcgtcaca cacaatcact tgtgtatgaa actgtgaaag
     4441 aggacgatgt aagcaaagag atcagggaaa gtgccgcgct tacgaaggcg gctttggcaa
     4501 gattttttgt tactgagacc gtcttatgac ggtttcggtc taggtttgat gtctttagac
     4561 atcatgaagg gccttgcgcc gttccagatt caggtacgat tacggacttg gagatgtggt
     4621 acgacgcttt gtttccggga aattcgttaa gagactcaag cctagacggg tatttggtgg
     4681 caacgactga ttgcaatttg cgattagaca atgttacgat caaaagtgga aactggaaag
     4741 acaagtttgc tgaaaaagaa acgtttctga aaccggttat tcgtactgct atgcctgaca
     4801 aaaggaagac tactcagttg gagagtttgt tagcattgca gaaaaggaac caagcggcac
     4861 ccgatctaca agaaaatgtg cacgcgacag ttctaatcga agagacgatg aagaagctga
     4921 aatctgttgt ctacgatgtg ggaaaaattc gggctgatcc tattgtcaat agagctcaaa
     4981 tggagagatg gtggagaaat caaagcacag cggtacaggc taaggtagta gcagatgtga
     5041 gagagttaca tgaaatagac tattcgtctt acatgtatat gatcaaatct gacgtgaaac
     5101 ctaagactga tttaacaccg caatttgaat actcagctct acagactgtt gtgtatcacg
     5161 agaagttgat caactcgttg ttcggtccaa ttttcaaaga aattaatgaa cgcaagttgg
     5221 atgctatgca accacatttt gtgttcaaca cgagaatgac atcgagtgat ttaaacgatc
     5281 gagtgaagtt cttaaatacg gaagcggctt acgactttgt tgagatagac atgtctaaat
     5341 tcgacaagtc ggcaaatcgc ttccatttac aactgcagct ggagatttac aggttatttg
     5401 ggctagatga gtgggcggcc ttcctttggg aggtgtcgca cactcaaact actgtgagag
     5461 atattcaaaa tggtatgatg gcgcatattt ggtaccaaca aaagagtgga gatgctgata
     5521 cttataatgc aaattcagat agaacactgt gtgcactctt gtctgaatta ccattggaga
     5581 aagcagtcat ggttacatat ggaggagatg actcactgat tgcgtttcct agaggaacgc
     5641 agtttgttga tccgtgtcca aagttggcta ctaagtggaa tttcgagtgc aagattttta
     5701 agtacgatgt cccaatgttt tgtgggaagt tcttgcttaa gacgtcatcg tgttacgagt
     5761 tcgtgccaga tccggtaaaa gttctgacga agttggggaa aaagagtata aaggatgtgc
     5821 aacatttagc cgagatctac atctcgctga atgattccaa tagagctctt gggaactaca
     5881 tggtggtatc caaactgtcc gagtctgttt cagaccggta tttgtacaaa ggtgattctg
     5941 ttcatgcgct ttgtgcgcta tggaagcata ttaagagttt tacagctctg tgtacattat
     6001 tccgagacga aaacgataag gaattgaacc cggctaaggt tgattggaag aaggcacaga
     6061 gagctgtgtc aaacttttac gactggtaat atggaagaca agtcattggt caccttgaag
     6121 aagaagactt tcgaagtctc aaaattctca aatctagggg ccattgaatt gtttgtggac
     6181 ggtaggagga agagaccgaa gtattttcac agaagaagag aaactgtcct aaatcatgtt
     6241 ggtgggaaga agagtgaaca caagttagac gtttttgacc aaagggatta caaaatgatt
     6301 aaatcttacg cgtttctaaa gatagtaggt gtacaactag ttgtaacatc acatctacct
     6361 gcagatacgc ctgggttcat tcaaatcgat ctgttggatt cgagacttac tgagaaaaga
     6421 aagagaggaa agactattca gagattcaaa gctcgagctt gcgataactg ttcagttgcg
     6481 cagtacaagg ttgaatacag tatttccaca caggagaacg tacttgatgt ctggaaggtg
     6541 ggttgtattt ctgagggcgt tccggtctgt gacggtacat accctttcag tatcgaagtg
     6601 tcgctaatat gggttgctac tgattcgact aggcgcctca atgtggaaga actgaacagt
     6661 tcggattaca ttgaaggcga ttttaccgat caagaggttt tcggtgagtt catgtctttg
     6721 aaacaagtgg agatgaagac gattgaggcg aagtacgatg gtccttacag accagctact
     6781 actagaccta agtcattatt gtcaggtgaa gatgttaaga gagcgcctaa taagaaaaac
     6841 tcgtcttaat gcataaagaa atttattgtc aatatgacgt gtgtactcaa gggttgtgtg
     6901 aatgaagtca ctgttcttgg tcacgagacg tgtagtatcg gtcatgctaa caaattgcga
     6961 aagcaagttg ctgacatggt tggtgtcaca cgtaggtgtg cggaaaataa ttgtggatgg
     7021 tttgtctgtg ttgttatcaa tgattttact tttgatgtgt ataattgttg tggccgtagt
     7081 caccttgaaa agtgtcgtaa acgtgttgaa acaagaaatc gagaaatttg gaaacaaatt
     7141 cgacgaaatc aagctgaaaa catgtctgcg acagctaaaa agtctcataa ttcgaagacc
     7201 tctaagaaga aattcaaaga ggacagagaa tttgggacac caaaaagatt tttaagagat
     7261 gatgttcctt tcgggattga tcgtttgttt gctttttgat tttattttat attgttatct
     7321 gtttctgtgt atagactgtt tgagattggc gcttggccga ctcattgtct taccataggg
     7381 gaacggactt tgtttgtgtt gttattttat ttgtatttta ttaaaattct caatgatctg
     7441 aaaaggcctc gaggctaaga gattattggg gggtgagtaa gtacttttaa agtgatgatg
     7501 gttacaaagg caaaaggggt aaaacccctc gcctacgtaa gcgttattac gcccgtctgt
     7561 acttatatca gtacactgac gagtccctaa aggacgaaac gggcccctcg aatttccccg
     7621 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga
     7681 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca
     7741 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg
     7801 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta
     7861 tgttactaga tcgggaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     7921 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     7981 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgcccgctcc
     8041 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     8101 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     8161 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     8221 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     8281 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg aaccaccatc
     8341 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag
     8401 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc
     8461 ccagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac
     8521 accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa
     8581 atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt
     8641 aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc
     8701 gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc
     8761 gttgctgcct gtgatcaaat atcatctccc tcgcagagat ccgaattatc agccttctta
     8821 ttcatttctc gcttaaccgt gacaggctgt cgatcttgag aactatgccg acataatagg
     8881 aaatcgctgg ataaagccgc tgaggaagct gagtggcgct atttctttag aagtgaacgt
     8941 tgacgatatc aactccccta tccattgctc accgaatggt acaggtcggg gacccgaagt
     9001 tccgactgtc ggcctgatgc atccccggct gatcgacccc agatctgggg ctgagaaagc
     9061 ccagtaagga aacaactgta ggttcgagtc gcgagatccc ccggaaccaa aggaagtagg
     9121 ttaaacccgc tccgatcagg ccgagccacg ccaggccgag aacattggtt cctgtaggca
     9181 tcgggattgg cggatcaaac actaaagcta ctggaacgag cagaagtcct ccggccgcca
     9241 gttgccaggc ggtaaaggtg agcagaggca cgggaggttg ccacttgcgg gtcagcacgg
     9301 ttccgaacgc catggaaacc gcccccgcca ggcccgctgc gacgccgaca ggatctagcg
     9361 ctgcgtttgg tgtcaacacc aacagcgcca cgcccgcagt tccgcaaata gcccccagga
     9421 ccgccatcaa tcgtatcggg ctacctagca gagcggcaga gatgaacacg accatcagcg
     9481 gctgcacagc gcctaccgtc gccgcgaccc cgcccggcag gcggtagacc gaaataaaca
     9541 acaagctcca gaatagcgaa atattaagtg cgccgaggat gaagatgcgc atccaccaga
     9601 ttcccgttgg aatctgtcgg acgatcatca cgagcaataa acccgccggc aacgcccgca
     9661 gcagcatacc ggcgacccct cggcctcgct gttcgggctc cacgaaaacg ccggacagat
     9721 gcgccttgtg agcgtccttg gggccgtcct cctgtttgaa gaccgacagc ccaatgatct
     9781 cgccgtcgat gtaggcgccg aatgccacgg catctcgcaa ccgttcagcg aacgcctcca
     9841 tgggcttttt ctcctcgtgc tcgtaaacgg acccgaacat ctctggagct ttcttcaggg
     9901 ccgacaatcg gatctcgcgg aaatcctgca cgtcggccgc tccaagccgt cgaatctgag
     9961 ccttaatcac aattgtcaat tttaatcctc tgtttatcgg cagttcgtag agcgcgccgt
    10021 gcgtcccgag cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt gttcctgaaa
    10081 tgccagtaaa gcgctggctg ctgaaccccc agccggaact gaccccacaa ggccctagcg
    10141 tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa
    10201 ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc
    10261 gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg agctgaaata
    10321 gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata tgaatttcgt
    10381 gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct ggcaacggga
    10441 cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg attccaggtg
    10501 cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc
    10561 ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa gctcgtagaa
    10621 cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca cctgctgcca
    10681 caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct tcacgtcctt
    10741 gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt
    10801 ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg
    10861 cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa
    10921 cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt
    10981 cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc
    11041 ctgttcgaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg cagggggagc
    11101 cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg agccgacgga
    11161 ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt cggcatcctc
    11221 ggcggaaaac cccgcgtcga tcagttcttg cctgtatgtc ttccggtcaa acgtccgatt
    11281 cattcaccct ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc cttattcctg
    11341 atttgacccg cctggtgcct tggtgtccag ataatccacc ttatcggcaa tgaagtcggt
    11401 cccgtagacc gtctggccgt ccttctcgta cttggtattc cgaatcttgc cctgcacgaa
    11461 taccagcgac cccttgccca aatacttgcc gtgggcctcg gcctgagagc caaaacactt
    11521 gatgcggaag aagtcggtgc gctcctgctt gtcgccggca tcgttgcgcc acatctaggt
    11581 actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc
    11641 ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc
    11701 ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa
    11761 agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa
    11821 agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt
    11881 taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta
    11941 agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt
    12001 cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt
    12061 gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc
    12121 tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc
    12181 atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg
    12241 tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca
    12301 ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata
    12361 ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca
    12421 agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa
    12481 ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac
    12541 ggagccgatt ttgaaaccac aattatgggt gatgctgcca acttactgat ttagtgtatg
    12601 atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc
    12661 tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcgct atctctgctc
    12721 tcactgccgt aaaacatggc aactgcagtt cacttacacc gcttctcaac ccggtacgca
    12781 ccagaaaatc attgatatgg ccatgaatgg cgttggatgc cgggcaacag cccgcattat
    12841 gggcgttggc ctcaacacga ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc
    12901 tcgcgcatac agccgggcag tgacgtcatc gtctgcgcgg aaatggacga acagtggggc
    12961 tatgtcgggg ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag tctccggaag
    13021 acggttgttg cgcacgtatt cggtgaacgc actatggcga cgctggggcg tcttatgagc
    13081 ctgctgtcac cctttgacgt ggtgatatgg atgacggatg gctggccgct gtatgaatcc
    13141 cgcctgaagg gaaagctgca cgtaatcagc aagcgatata cgcagcgaat tgagcggcat
    13201 aacctgaatc tgaggcagca cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa
    13261 tcggtggagc tgcatgacaa agtcatcggg cattatctga acataaaaca ctatcaataa
    13321 gttggagtca ttacccaatt atgatagaat ttacaagcta taaggttatt gtcctgggtt
    13381 tcaagcatta gtccatgcaa gtttttatgc tttgcccatt ctatagatat attgataagc
    13441 gcgctgccta tgccttgccc cctgaaatcc ttacatacgg cgatatcttc tatataaaag
    13501 atatattatc ttatcagtat tgtcaatata ttcaaggcaa tctgcctcct catcctcttc
    13561 atcctcttcg tcttggtagc tttttaaata tggcgcttca tagagtaatt ctgtaaaggt
    13621 ccaattctcg ttttcatacc tcggtataat cttacctatc acctcaaatg gttcgctggg
    13681 tttatcgcac ccccgaacac gagcacggca cccgcgacca ctatgccaag aatgcccaag
    13741 gtaaaaattg ccggccccgc catgaagtcc gtgaatgccc cgacggccga agtgaagggc
    13801 aggccgccac ccaggccgcc gccctcactg cccggcacct ggtcgctgaa tgtcgatgcc
    13861 agcacctgcg gcacgtcaat gcttccgggc gtcgcgctcg ggctgatcgc ccatcccgtt
    13921 actgccccga tcccggcaat ggcaaggact gccagcgctg ccatttttgg ggtgaggccg
    13981 ttcgcggccg aggggcgcag cccctggggg gatgggaggc ccgcgttagc gggccgggag
    14041 ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc
    14101 ctggttaaaa acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg
    14161 gtggccgaaa aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc
    14221 ctcaaatgtc aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga
    14281 tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat
    14341 ccccaggctt gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat
    14401 ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa
    14461 cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag
    14521 ggccaagttt tccgcgaggt atccacaacg ccggcggccg cggtgtctcg cacacggctt
    14581 cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag cggcgagggc
    14641 aaccagcccg gtgagcgtcg gaaaggcgct cggtcttgcc ttgctcgtcg gtgatgtact
    14701 tcaccagctc cgcgaagtcg ctcttcttga tggagcgcat ggggacgtgc ttggcaatca
    14761 cgcgcacccc ccggccgttt tagcggctaa aaaagtcatg gctctgccct cgggcggacc
    14821 acgcccatca tgaccttgcc aagctcgtcc tgcttctctt cgatcttcgc cagcagggcg
    14881 aggatcgtgg catcaccgaa ccgcgccgtg cgcgggtcgt cggtgagcca gagtttcagc
    14941 aggccgccca ggcggcccag gtcgccattg atgcgggcca gctcgcggac gtgctcatag
    15001 tccacgacgc ccgtgatttt gtagccctgg ccgacggcca gcaggtaggc cgacaggctc
    15061 atgccggccg ccgccgcctt ttcctcaatc gctcttcgtt cgtctggaag gcagtacacc
    15121 ttgataggtg ggctgccctt cctggttggc ttggtttcat cagccatccg cttgccctca
    15181 tctgttacgc cggcggtagc cggccagcct cgcagagcag gattcccgtt gagcaccgcc
    15241 aggtgcgaat aagggacagt gaagaaggaa cacccgctcg cgggtgggcc tacttcacct
    15301 atcctgcccg gctgacgccg ttggatacac caaggaaagt ctacacgaac cctttggcaa
    15361 aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat aatgaccccg
    15421 aagcagggtt atgcagcgga aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    15481 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    15541 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    15601 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    15661 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
    15721 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
    15781 gcgcagcgag tcagtgagcg aggaagcgga agagcgccag aaggccgcca gagaggccga
    15841 gcgcggccgt gaggcttgga cgctagggca gggcatgaaa aagcccgtag cgggctgcta
    15901 cgggcgtctg acgcggtgga aagggggagg ggatgttgtc tacatggctc tgctgtagtg
    15961 agtgggttgc gctccggcag cggtcctgat caatcgtcac cctttctcgg tccttcaacg
    16021 ttcctgacaa cgagcctcct tttcgccaat ccatcgacaa tcaccgcgag tccctgctcg
    16081 aacgctgcgt ccggaccggc ttcgtcgaag gcgtctatcg cggcccgcaa cagcggcgag
    16141 agcggagcct gttcaacggt gccgccgcgc tcgccggcat cgctgtcgcc ggcctgctcc
    16201 tcaagcacgg ccccaacagt gaagtagctg attgtcatca gcgcattgac ggcgtccccg
    16261 gccgaaaaac ccgcctcgca gaggaagcga agctgcgcgt cggccgtttc catctgcggt
    16321 gcgcccggtc gcgtgccggc atggatgcgc gcgccatcgc ggtaggcgag cagcgcctgc
    16381 ctgaagctgc gggcattccc gatcagaaat gagcgccagt cgtcgtcggc tctcggcacc
    16441 gaatgcgtat gattctccgc cagcatggct tcggccagtg cgtcgagcag cgcccgcttg
    16501 ttcctgaagt gccagtaaag cgccggctgc tgaaccccca accgttccgc cagtttgcgt
    16561 gtcgtcagac cgtctacgcc gacctcgttc aacaggtcca gggcggcacg gatcactgta
    16621 ttcggctgca actttgtcat gcttgacact ttatcactga taaacataat atgtccacca
    16681 acttatcagt gataaagaat ccgcgcgttc aatcggacca gcggaggctg gtccggaggc
    16741 cagacgtgaa acccaacata cccctgatcg taattctgag cactgtcgcg ctcgacgctg
    16801 tcggcatcgg cctgattatg ccggtgctgc cgggcctcct gcgcgatctg gttcactcga
    16861 acgacgtcac cgcccactat ggcattctgc tggcgctgta tgcgttggtg caatttgcct
    16921 gcgcacctgt gctgggcgcg ctgtcggatc gtttcgggcg gcggccaatc ttgctcgtct
    16981 cgctggccgg cgccagatct ggggaaccct gtggttggca tgcacataca aatggacgaa
    17041 cggataaacc ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc
    17101 ttaggtttac ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa
    17161 cgacaatctg atcatgagcg gagaattaag ggagtcacgt tatgaccccc gccgatgacg
    17221 cgggacaagc cgttttacgt ttggaactga cagaaccgca acgttgaagg agccactcag
    17281 ccgcgggttt ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt
    17341 caaaagtcgc ctaaagctag cttgcatgcc tgcaggtcac tattccagta tggacgattc
    17401 aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaag aaagtagttc
    17461 ctactgaatc aaaggccatg gagtcaaaaa ttcagatcga ggatctaaca gaactcgccg
    17521 tgaagactgg cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct
    17581 tcgtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
    17641 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
    17701 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
    17761 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc cctgccgaca
    17821 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
    17881 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
    17941 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
    18001 ggacacgctg aaatcaccag tctctctcta caaatcggat ccatgagccc agaacgacgc
    18061 ccggccgaca tccgccgtgc caccgaggcg gacatgccgg cggtctgcac catcgtcaac
    18121 cactacatcg agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc gcaggagtgg
    18181 acggacgacc tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga ggtggacggc
    18241 gaggtcgccg gcatcgccta cgcgggcccc tggaaggcac gcaacgccta cgactggacg
    18301 gccgagtcga ccgtgtacgt ctccccccgc caccagcgga cgggactggg ctccacgctc
    18361 tacacccacc tgctgaagtc cctggaggca cagggcttca agagcgtggt cgctgtcatc
    18421 gggctgccca acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc cccccgcggc
    18481 atgctgcggg cggccggctt caagcacggg aactggcatg acgtgggttt ctggcagctg
    18541 gacttcagcc tgccggtacc gccccgtccg gtcctgcccg tcaccgagat ctgagatcac
    18601 gcggactcta gaggatcccc cgatgagcta agctagctat atcatcaatt tatgtattac
    18661 acataatatc gcactcagtc tttcatctac ggcaatgtac cagctgatat aatcagttat
    18721 tgaaatattt ctgaatttaa acttgcatca ataaatttat gtttttgctt ggactataat
    18781 acctgacttg ttattttatc aataaatatt taaactatat ttctttcaag atgggaattc
    18841 atcgatggtt tcattggtga cgtttccggc cttgctaatg gtaatggtgc tactggtgat
    18901 tttgctggct ctaattccca aatggctcaa gtcggtgacg gtgataattc acctttaatg
    18961 aataatttcc gtcaatattt accttccctc cctcaatcgg ttgaatgtcg cccttttgtc
    19021 tttggcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
    19081 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
    19141 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
    19201 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
    19261 atgggacgtc gacctgaggt aattataacc cgggccctat atatggatcc aattgcaatg
    19321 atcatcatga cagatctgcg cgcgatcgat atcagcgctt taaatttgc
//
