LOCUS       sequence-471203-       12353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ISYmu1 PDS3 gRNA2 TRV2 Architecture_A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12353)
  AUTHORS   Weiss T, Kamalu M, Shi H, Li Z, Amerasekera J, Zhong Z, Adler BA, 
            Song MM, Vohra K, Wirnowski G, Chitkara S, Ambrose C, Steinmetz N, 
            Sridharan A, Sahagun D, Banfield JF, Doudna JA, Jacobsen SE
  TITLE     Viral delivery of an RNA-guided genome editor for transgene-free 
            germline editing in Arabidopsis.
  JOURNAL   Nat Plants. 2025 Apr 22. doi: 10.1038/s41477-025-01989-9.
  PUBMED    40263581
REFERENCE   2  (bases 1 to 12353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2025 Apr 22. doi: 10.1038/s41477-025-01989-9."
COMMENT     Sequence Label: pTW2065
FEATURES             Location/Qualifiers
     source          1..12353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1766..1790
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(1812..2064)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2099..2116)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(3976..3996)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(4003..4050)
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     promoter        complement(5627..5972)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(5664..5684)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    6501..6525
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6950..7744
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9169..9757
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9658..9677
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9911..9928
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9943..10083
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10009..10028)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10169..10191
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10427..10621
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10687..11760)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(join(12189..12353,1..465))
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc
       61 cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc
      121 ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt
      181 gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg
      241 ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag
      301 cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac
      361 taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt
      421 cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt
      481 atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac
      541 tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc
      601 ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt
      661 gagacgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct
      721 tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt
      781 ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg
      841 caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca
      901 gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt
      961 cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact
     1021 tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac
     1081 gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg
     1141 tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg
     1201 caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa
     1261 cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc
     1321 cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt
     1381 tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc
     1441 tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa
     1501 aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg
     1561 acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac
     1621 tatagcagcg gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc
     1681 atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat
     1741 tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata
     1801 gtttaattcc cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc
     1861 gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa
     1921 aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt
     1981 caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact
     2041 ttattgccaa atgtttgaac gatcggggaa attcgagctg gtcacctgta attcacacgt
     2101 ggtggtggtg gtggtggcta gcgttctccc gtttcgtcct ttagggactc gtcagtgtac
     2161 tgatataagt acagacgggc gtaataacgc ttacgtaggc gaggggtttt accccttttg
     2221 cctttgtaac catcatcact ttaaaagtac ttactcaccc cccaacaatc tcttagccgc
     2281 gaggcttttt cagatcgttg agaattttaa taaaatacaa ataaaataac aacacaaaca
     2341 aagtccgttc ccctatggta agacaatgag tcggccaaac gccgatctca aacagtctat
     2401 acacagaaac agataacaat ttaaaataaa atcaaaaaga aaattaaatt acaaaagact
     2461 taccgatcaa tcaagatcag tcgagaatgt caatctcgta ggtttaaatt gaacctaaaa
     2521 cttcagacac ggatctactt aaagaaccgt agtttaatgt cttcgggaca tgcccgggtg
     2581 cttccggcgg ggctcgaacc cgcgaccttc ggctcataag accaacgctc taaccaactg
     2641 agctacgggc ggcgaatttg ccttttgaaa ctctcatgac taaagtcacg agattcttgg
     2701 gaacagagca tacttaccgg cttatttctt tgccggcaga ggtttctctt gtttaccaag
     2761 ctacccccgc aattcctgcg gttcctgttt gcttctgtat tgctaagacc ccggagtcag
     2821 agctcttatc ctgagtccct cagccagtat atttcttgct gcattgacgt cacggtcatg
     2881 gtgggcatga caaacaggac aggtccactc ccttacatgg agaggcttct tcctgtcctt
     2941 gtggccgcac tctgagcata tctgacttga cggaaaccat ctgcttatcc tgatgacttc
     3001 ttttccatac caggaagcct tgtactgcag tttcgataca aggctcgtcc atgatacatc
     3061 agagatgctt tttgccagtt tatgattgcg catcatgccc ttaacgttaa ggtcctcgat
     3121 acagatgata tcgtggtttt tgactatctc tgtactgagt ttgttaagat attcttttcg
     3181 ctggtttgca accttttcat aaagtctcgc taccttccgc ctttgcttct gatagttcct
     3241 ggcttcagaa agggaaatgc cccttttctc cgcagcaagt gcacgccttg caagctttcg
     3301 ctgctcccgt ctaagccttt cttccatttg cctggtaaaa tgattgttgt catgcctgct
     3361 tccgtctgac ataaccgcaa agtcaatgat tccgaggtca attccgacag atgagtcagt
     3421 ctttggcaat tcacagatct cctcttcgca taggatcgat acgaagaatt tcccgctcga
     3481 tttccttcgt actgtggcat tcagtatacg gcctttcatc tccctactgt ctgcaaactt
     3541 gacaaggccc agcttaggca gcttcagaca atttccggaa actgcgatgt tgttgttcgt
     3601 atattgtgtc gtgtagcttt gcctcgggct cttcttgctc ttgaactggg gatgctcgtt
     3661 ctgtcctttg aaaaagcggg agaaggcgtc cgacaggtta cgaagcgaat tctggagcga
     3721 gaacttatcc acttcacaaa gccatactgt ttccgggtcc ctcttaagct ttgtcagaag
     3781 tttggagcat gcgtaataag tcagaccttt tccggtctcc tcatattcct tgttccaaag
     3841 ttccaggaaa tggttgtata cataccttga actgcctatt gtcttggcaa tcagtgtttc
     3901 ctgcttctta tctggataga tacggtattc ataggcttta tgctgcagca tagcagctgg
     3961 aactccgtgg attccaacct ttctcttctt ctttggagcc atcttatcat catcatcctt
     4021 gtaatcctta tcatcatcat ccttgtaatc catctcgtta actcgggtaa gtgatacagt
     4081 gaccagtagg tcagcaaacc gagttgtcaa atttttaata acagaatgat agactctatc
     4141 aacgagagac aaatcagtat aattatgctc ttaattaaga taacttaatt aacttaacac
     4201 tttctctaaa gatagtgtga aaccccagaa caagatgctc agcctgttct tgcgcatgta
     4261 ttactacagc gtttttaacc ttgtgtgccg aaactcaaat gctaccaacg acactgacgt
     4321 caatggtaaa ggaaattccc tcaaattagt cacaattagc cctatttaga tgtcgctaat
     4381 ggcatcagtg aatatggtat cacccaccct ctgaatttca cagagatctc aacagagttc
     4441 acgtccttaa atccctaggg attaggacgt atcggacctc cactcgctgg aggcggtgca
     4501 gatgaactag cagctgctcc accaaactta atcccgaata ctttctcaaa gttccttcgg
     4561 taataactct tcaactccat gttctctaac gaagttagag atttgtggaa agctgtagag
     4621 gcatcatcga atttgtaata agtgtacaag tcttcttcca aagtcgagcc agtgttcgcc
     4681 ttggtagtag tagtactccc ttggttcgtc gtaaccgttg tgtttggatt cgcaggaata
     4741 tttgtaacac ccgccactgc attcttctca tcagctcgtc tcttgaacgc tgaagcgttc
     4801 tgaatcacac caagagcact gttagcccgt gagtctaaaa ggaaatacac cttcttatct
     4861 ggaaactgct catatggtga ataaggacac tgtctattca gtgccgctag taacccagtg
     4921 atctcatctc taccagactg tagtgcaaat tttatgctgt gtaacttctt caacagatct
     4981 ttccacgaaa ctgattccac ccaagaatcg tccatcaagt cagcaggacc gcccgacttg
     5041 tcaaatgatt catcgtacat atctcccatg ttgattagcg caagtgattc agtaacctgt
     5101 agttcaacaa gtctaatcgt ccaaattaaa atgatcttat cagcagacac aagtattata
     5161 tttatgccat ctctaatgat aacattaaag acttagctag ttttctctaa agaaactagc
     5221 aaaaccccaa cgtccgttta gacgcttgcg taggagaagt taattaaaac gaccccatcg
     5281 tttttgcggt agcgattaaa accaccacct ttctttcatt gctactttcg taagcgatac
     5341 gtctcaggtt ttcctttgta tgagaagaca agtgctttct tactaaccta aagaaccgaa
     5401 acccaaagta acatttcaaa gataagcaca ccagtcactt tcccgtacgg tactaagctg
     5461 gatagttcaa aaagaaatat tacactgaca aaaacctcgt cagggagagg gataaaggct
     5521 gtgttggata aggacgttct accgtattct ccaacttgtc aattagtaca ttctactgcg
     5581 atcactgaca taccccagcc agggcaacac cataggtgca atgttttatc ctctccaaat
     5641 gaaatgaact tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat
     5701 cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt
     5761 cttctttttc cacgatgttc ctcgtgggtg ggggtccatc tttgggacca ctgtcggtag
     5821 aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag aagccatctt
     5881 ccttttctac tgtcctttcg atgaagtgac agatagctgg gcaatggaat ccgaggaggt
     5941 ttcccgatat taccctttgt tgaaaagtct caatagccct ctggtcttct gagactgtat
     6001 ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttgacc atcacatcaa
     6061 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgtt cctcgtgggt
     6121 gggggtccat ctttgggacc actgtcggta gaggcatctt gaacgatagc ctttccttta
     6181 tcgcaatgat ggcatttgta gaagccatct tccttttcta ctgtcctttc gatgaagtga
     6241 cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc
     6301 tcaatagccc tctggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg
     6361 tcgtgctcca ccatgttgac ctgcaggcat gcaagcttgg cgcgccgggc ccaacatggt
     6421 ggcctaggaa ttaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta
     6481 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag
     6541 ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg
     6601 gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta
     6661 ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
     6721 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaccgcggt ttcaaaatcg
     6781 gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct
     6841 ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag
     6901 cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa tggctaaaat
     6961 gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga
     7021 aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa
     7081 aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat
     7141 gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg
     7201 ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga
     7261 tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc
     7321 catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga
     7381 ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag aagacactcc
     7441 atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact
     7501 tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag
     7561 tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg
     7621 cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt
     7681 actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt
     7741 ttagtaccta gaatgcatga ccaaaatccc ttaacgctga gagatcccct cataatttcc
     7801 ccaaaacgta accatgtgtg aatagatttt gagtaagcag ggttgcagcc acgagtgagt
     7861 cttcccttgt tattgtgtag ccagaatgcc gcaaaacttc catgcctaag cgaactgttg
     7921 agagtacgtt tcgatttctg actgtgttag cctggaagtg cttgtcccaa ccttgtttct
     7981 gagcatgaac gcccgcaagc caacatgtta gttgaagcat cagggcgatt agcagcatga
     8041 tatcaaaacg ctctgagctg ctcgttcggc tatggcgtag gcctagtccg taggcaggac
     8101 ttttcaagtc tcggaaggtt tcttcaatct gcattcgctt cgaatagata ttaacaagtt
     8161 gtttgggtgt tcgaatttca acaggtaagt tagttgctag aatccatggc tcctttgccg
     8221 acgctgagta gattttaggt gacgggtggt gacaatgagt ccgtgtcgag cgctgatttt
     8281 ttcggccttt agagcgagat ttatacaata gaatttggca tgagattgga ttgcttttag
     8341 tcagcctctt atagcctaaa gtctttgagt gactagatga catatcatgt aagttgctga
     8401 taggtttcca gttttccgct cctaggtctg catattgtac ttttcctctt actcgactta
     8461 accagtacca acccagcttc tcaacggatt tataccatgg cactttaaag ccagcatcac
     8521 tgacaatgag cggtgtggtg ttactcggta gaatgctcgc aaggtcggct agaaattggt
     8581 catgagcttt ctttgaacat tgctctgaaa gcgggaacgc tttctcataa agagtaacag
     8641 aacgaccgtg tagtgcgact gaagctcgca ataccataag ccgtttttgc tcacggatat
     8701 cagaccagtc aacaagtaca atgggcatcg tattgcccga acagataaag ctagcatgcc
     8761 aacggtatac agcgagtcgc tctttgtgga ggtgacgatt acctaacaat cggtcgattc
     8821 gtttgatgtt atgttttgtt ctcgctttgg ttggcaggtt acggccaagt tcggtaagag
     8881 tgagagtttt acagtcaagt aaggcgtggc aagccaacgt taagctgttg agtcgtttta
     8941 agtgtaattc ggggcagaat tggtaaagag agtcgtgtaa aatatcgagt tcgcacattt
     9001 tgttgtctga ttattgattt ttggcgaaac catttgatca tatgacaaga tgtgtatcta
     9061 ccttaactta atgattttga taaaaatcat taggggattc atcagccctt aacgtgagtt
     9121 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     9181 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     9241 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     9301 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     9361 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     9421 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     9481 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     9541 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9601 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9661 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9721 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9781 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9841 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9901 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
     9961 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
    10021 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
    10081 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
    10141 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
    10201 tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga
    10261 cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg
    10321 gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag
    10381 ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag
    10441 gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt
    10501 ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc
    10561 aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac
    10621 aggaaagaga ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc
    10681 cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag
    10741 gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc
    10801 gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc
    10861 gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg
    10921 gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac
    10981 gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat
    11041 gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc
    11101 ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg
    11161 cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc
    11221 tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc
    11281 tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac
    11341 caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc
    11401 gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa
    11461 aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac
    11521 gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc
    11581 cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg
    11641 gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac
    11701 caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgctcac
    11761 gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcaggc aacccagccg
    11821 cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct
    11881 ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat
    11941 tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt
    12001 cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa
    12061 gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct
    12121 gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc
    12181 tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca
    12241 gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag
    12301 ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc cag
//
