LOCUS       sequence-471204-       12421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ISYmu1 CHLl1 gRNA9 TRV2 Architecture_B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12421)
  AUTHORS   Weiss T, Kamalu M, Shi H, Li Z, Amerasekera J, Zhong Z, Adler BA, 
            Song MM, Vohra K, Wirnowski G, Chitkara S, Ambrose C, Steinmetz N, 
            Sridharan A, Sahagun D, Banfield JF, Doudna JA, Jacobsen SE
  TITLE     Viral delivery of an RNA-guided genome editor for transgene-free 
            germline editing in Arabidopsis.
  JOURNAL   Nat Plants. 2025 Apr 22. doi: 10.1038/s41477-025-01989-9.
  PUBMED    40263581
REFERENCE   2  (bases 1 to 12421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2025 Apr 22. doi: 10.1038/s41477-025-01989-9."
COMMENT     Sequence Label: pTW2159
FEATURES             Location/Qualifiers
     source          1..12421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             375..1448
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      1514..1708
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1944..1966)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2052..2192
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2107..2126
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2207..2224)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2378..2966)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2458..2477)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4391..5185)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    5610..5634
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        6163..6508
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     6451..6471
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             8085..8132
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             8139..8159
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     ncRNA           9487..9554
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     CDS             10087..10104
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      10139..10391
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10413..10437
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11738..12367
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca
       61 tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg
      121 tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt
      181 aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa
      241 aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt
      301 tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg aacccccaag
      361 cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc
      421 tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg
      481 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat
      541 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc
      601 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca
      661 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct
      721 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt
      781 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc
      841 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca
      901 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc
      961 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg
     1021 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg
     1081 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag
     1141 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg
     1201 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt
     1261 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg
     1321 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc
     1381 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg
     1441 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa
     1501 aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc
     1561 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga
     1621 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa
     1681 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc
     1741 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta
     1801 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg
     1861 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg
     1921 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     1981 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     2041 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     2101 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     2161 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     2221 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     2281 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2341 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2401 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2461 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2521 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2581 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2641 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2701 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2761 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     2821 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2881 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2941 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3001 ctcagtggaa cgaaaactca cgttaagggc tgatgaatcc cctaatgatt tttatcaaaa
     3061 tcattaagtt aaggtagata cacatcttgt catatgatca aatggtttcg ccaaaaatca
     3121 ataatcagac aacaaaatgt gcgaactcga tattttacac gactctcttt accaattctg
     3181 ccccgaatta cacttaaaac gactcaacag cttaacgttg gcttgccacg ccttacttga
     3241 ctgtaaaact ctcactctta ccgaacttgg ccgtaacctg ccaaccaaag cgagaacaaa
     3301 acataacatc aaacgaatcg accgattgtt aggtaatcgt cacctccaca aagagcgact
     3361 cgctgtatac cgttggcatg ctagctttat ctgttcgggc aatacgatgc ccattgtact
     3421 tgttgactgg tctgatatcc gtgagcaaaa acggcttatg gtattgcgag cttcagtcgc
     3481 actacacggt cgttctgtta ctctttatga gaaagcgttc ccgctttcag agcaatgttc
     3541 aaagaaagct catgaccaat ttctagccga ccttgcgagc attctaccga gtaacaccac
     3601 accgctcatt gtcagtgatg ctggctttaa agtgccatgg tataaatccg ttgagaagct
     3661 gggttggtac tggttaagtc gagtaagagg aaaagtacaa tatgcagacc taggagcgga
     3721 aaactggaaa cctatcagca acttacatga tatgtcatct agtcactcaa agactttagg
     3781 ctataagagg ctgactaaaa gcaatccaat ctcatgccaa attctattgt ataaatctcg
     3841 ctctaaaggc cgaaaaaatc agcgctcgac acggactcat tgtcaccacc cgtcacctaa
     3901 aatctactca gcgtcggcaa aggagccatg gattctagca actaacttac ctgttgaaat
     3961 tcgaacaccc aaacaacttg ttaatatcta ttcgaagcga atgcagattg aagaaacctt
     4021 ccgagacttg aaaagtcctg cctacggact aggcctacgc catagccgaa cgagcagctc
     4081 agagcgtttt gatatcatgc tgctaatcgc cctgatgctt caactaacat gttggcttgc
     4141 gggcgttcat gctcagaaac aaggttggga caagcacttc caggctaaca cagtcagaaa
     4201 tcgaaacgta ctctcaacag ttcgcttagg catggaagtt ttgcggcatt ctggctacac
     4261 aataacaagg gaagactcac tcgtggctgc aaccctgctt actcaaaatc tattcacaca
     4321 tggttacgtt ttggggaaat tatgagggga tctctcagcg ttaagggatt ttggtcatgc
     4381 attctaggta ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag
     4441 gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc
     4501 tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa
     4561 gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc
     4621 cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc
     4681 gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt
     4741 tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat
     4801 ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt
     4861 cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga
     4921 gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc
     4981 cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc
     5041 ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag
     5101 caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt
     5161 ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa
     5221 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
     5281 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
     5341 agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg
     5401 ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt
     5461 gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc
     5521 ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc
     5581 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg
     5641 acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa
     5701 ttaattccta ggccaccatg ttgggcccgg cgcgccaagc ttgcatgcct gcaggtcaac
     5761 atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac
     5821 cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat
     5881 tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa
     5941 tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc
     6001 aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct
     6061 tcaaagcaag tggattgatg tgatggtcaa catggtggag cacgacactc tcgtctactc
     6121 caagaatatc aaagatacag tctcagaaga ccagagggct attgagactt ttcaacaaag
     6181 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag
     6241 gacagtagaa aaggaagatg gcttctacaa atgccatcat tgcgataaag gaaaggctat
     6301 cgttcaagat gcctctaccg acagtggtcc caaagatgga cccccaccca cgaggaacat
     6361 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     6421 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     6481 aggaagttca tttcatttgg agaggataaa acattgcacc tatggtgttg ccctggctgg
     6541 ggtatgtcag tgatcgcagt agaatgtact aattgacaag ttggagaata cggtagaacg
     6601 tccttatcca acacagcctt tatccctctc cctgacgagg tttttgtcag tgtaatattt
     6661 ctttttgaac tatccagctt agtaccgtac gggaaagtga ctggtgtgct tatctttgaa
     6721 atgttacttt gggtttcggt tctttaggtt agtaagaaag cacttgtctt ctcatacaaa
     6781 ggaaaacctg agacgtatcg cttacgaaag tagcaatgaa agaaaggtgg tggttttaat
     6841 cgctaccgca aaaacgatgg ggtcgtttta attaacttct cctacgcaag cgtctaaacg
     6901 gacgttgggg ttttgctagt ttctttagag aaaactagct aagtctttaa tgttatcatt
     6961 agagatggca taaatataat acttgtgtct gctgataaga tcattttaat ttggacgatt
     7021 agacttgttg aactacaggt tactgaatca cttgcgctaa tcaacatggg agatatgtac
     7081 gatgaatcat ttgacaagtc gggcggtcct gctgacttga tggacgattc ttgggtggaa
     7141 tcagtttcgt ggaaagatct gttgaagaag ttacacagca taaaatttgc actacagtct
     7201 ggtagagatg agatcactgg gttactagcg gcactgaata gacagtgtcc ttattcacca
     7261 tatgagcagt ttccagataa gaaggtgtat ttccttttag actcacgggc taacagtgct
     7321 cttggtgtga ttcagaacgc ttcagcgttc aagagacgag ctgatgagaa gaatgcagtg
     7381 gcgggtgtta caaatattcc tgcgaatcca aacacaacgg ttacgacgaa ccaagggagt
     7441 actactacta ccaaggcgaa cactggctcg actttggaag aagacttgta cacttattac
     7501 aaattcgatg atgcctctac agctttccac aaatctctaa cttcgttaga gaacatggag
     7561 ttgaagagtt attaccgaag gaactttgag aaagtattcg ggattaagtt tggtggagca
     7621 gctgctagtt catctgcacc gcctccagcg agtggaggtc cgatacgtcc taatccctag
     7681 ggatttaagg acgtgaactc tgttgagatc tctgtgaaat tcagagggtg ggtgatacca
     7741 tattcactga tgccattagc gacatctaaa tagggctaat tgtgactaat ttgagggaat
     7801 ttcctttacc attgacgtca gtgtcgttgg tagcatttga gtttcggcac acaaggttaa
     7861 aaacgctgta gtaatacatg cgcaagaaca ggctgagcat cttgttctgg ggtttcacac
     7921 tatctttaga gaaagtgtta agttaattaa gttatcttaa ttaagagcat aattatactg
     7981 atttgtctct cgttgataga gtctatcatt ctgttattaa aaatttgaca actcggtttg
     8041 ctgacctact ggtcactgta tcacttaccc gagttaacga gatggattac aaggatgatg
     8101 atgataagga ttacaaggat gatgatgata agatggctcc aaagaagaag agaaaggttg
     8161 gaatccacgg agttccagct gctatgctgc agcataaagc ctatgaatac cgtatctatc
     8221 cagataagaa gcaggaaaca ctgattgcca agacaatagg cagttcaagg tatgtataca
     8281 accatttcct ggaactttgg aacaaggaat atgaggagac cggaaaaggt ctgacttatt
     8341 acgcatgctc caaacttctg acaaagctta agagggaccc ggaaacagta tggctttgtg
     8401 aagtggataa gttctcgctc cagaattcgc ttcgtaacct gtcggacgcc ttctcccgct
     8461 ttttcaaagg acagaacgag catccccagt tcaagagcaa gaagagcccg aggcaaagct
     8521 acacgacaca atatacgaac aacaacatcg cagtttccgg aaattgtctg aagctgccta
     8581 agctgggcct tgtcaagttt gcagacagta gggagatgaa aggccgtata ctgaatgcca
     8641 cagtacgaag gaaatcgagc gggaaattct tcgtatcgat cctatgcgaa gaggagatct
     8701 gtgaattgcc aaagactgac tcatctgtcg gaattgacct cggaatcatt gactttgcgg
     8761 ttatgtcaga cggaagcagg catgacaaca atcattttac caggcaaatg gaagaaaggc
     8821 ttagacggga gcagcgaaag cttgcaaggc gtgcacttgc tgcggagaaa aggggcattt
     8881 ccctttctga agccaggaac tatcagaagc aaaggcggaa ggtagcgaga ctttatgaaa
     8941 aggttgcaaa ccagcgaaaa gaatatctta acaaactcag tacagagata gtcaaaaacc
     9001 acgatatcat ctgtatcgag gaccttaacg ttaagggcat gatgcgcaat cataaactgg
     9061 caaaaagcat ctctgatgta tcatggacga gccttgtatc gaaactgcag tacaaggctt
     9121 cctggtatgg aaaagaagtc atcaggataa gcagatggtt tccgtcaagt cagatatgct
     9181 cagagtgcgg ccacaaggac aggaagaagc ctctccatgt aagggagtgg acctgtcctg
     9241 tttgtcatgc ccaccatgac cgtgacgtca atgcagcaag aaatatactg gctgagggac
     9301 tcaggataag agctctgact ccggggtctt agcaatacag aagcaaacag gaaccgcagg
     9361 aattgcgggg gtagcttggt aaacaagaga aacctctgcc ggcaaagaaa taagccggta
     9421 agtatgctct gttcccaaga atctcgtgac tttagtcatg agagtttcaa cggtttggta
     9481 tgcatgggcc ggcatggtcc cagcctcctc gctggcgccg gctgggcaac atgcttcggc
     9541 atggcgaatg ggacccgtag ctcagttggt tagagcgttg gtcttatgag ccgaaggtcg
     9601 cgggttcgag ccccgccgga agcacccggg catgtcccga agacattaaa ctacggttct
     9661 ttaagtagat ccgtgtctga agttttaggt tcaatttaaa cctacgagat tgacattctc
     9721 gactgatctt gattgatcgg taagtctttt gtaatttaat tttctttttg attttatttt
     9781 aaattgttat ctgtttctgt gtatagactg tttgagatcg gcgtttggcc gactcattgt
     9841 cttaccatag gggaacggac tttgtttgtg ttgttatttt atttgtattt tattaaaatt
     9901 ctcaacgatc tgaaaaagcc tcgcggctaa gagattgttg gggggtgagt aagtactttt
     9961 aaagtgatga tggttacaaa ggcaaaaggg gtaaaacccc tcgcctacgt aagcgttatt
    10021 acgcccgtct gtacttatat cagtacactg acgagtccct aaaggacgaa acgggagaac
    10081 gctagccacc accaccacca ccacgtgtga attacaggtg accagctcga atttccccga
    10141 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
    10201 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
    10261 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
    10321 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
    10381 gttactagat cgggaattaa actatcagtg tttgacagga tatattggcg ggtaaaccta
    10441 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc
    10501 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg
    10561 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct
    10621 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc
    10681 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg
    10741 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc
    10801 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag
    10861 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt
    10921 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc
    10981 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc
    11041 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc
    11101 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag
    11161 gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc
    11221 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg
    11281 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc
    11341 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg
    11401 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg
    11461 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt
    11521 tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg
    11581 atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc
    11641 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga
    11701 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa
    11761 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact
    11821 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc
    11881 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg
    11941 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc
    12001 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag
    12061 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt
    12121 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat
    12181 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat
    12241 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga
    12301 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact
    12361 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc
    12421 c
//
