LOCUS       sequence-471207-       10318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation SIV-based lentiviral packaging plasmid; Contains SIV 
            Gag and Pol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Benedetti L, Fan R, Weigel AV, Moore AS, Houlihan PR, Kittisopikul 
            M, Park G, Petruncio A, Hubbard PM, Pang S, Xu CS, Hess HF, Saalfeld
            S, Rangaraju V, Clapham DE, De Camilli P, Ryan TA, 
            Lippincott-Schwartz J
  TITLE     Periodic ER-plasma membrane junctions support long-range Ca(2+) 
            signal integration in dendrites.
  JOURNAL   Cell. 2025 Jan 23;188(2):484-500.e22. doi: 
            10.1016/j.cell.2024.11.029. Epub 2024 Dec 20.
  PUBMED    39708809
REFERENCE   2  (bases 1 to 10318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.11.029"; journalName: "Cell"; date: 
            "2025-01-23- 23"; volume: "188"; issue: "2"; pages: "484-500.e22"
COMMENT     Sequence Label: SIV_Gag_Pol
FEATURES             Location/Qualifiers
     source          1..10318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     primer_bind     complement(5954..5973)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5981..6997
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(6035..6057)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6998..7273)
                     /label=chicken beta-actin promoter
     enhancer        7275..7654
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7685..7789
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7790..7858,7859..8650)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8008..8027)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8821..9409
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9310..9329
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9563..9580
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    9648..9782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9672..9691)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9726..9745
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(9788..9983)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9802..9937
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    10042..10063
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10078..10083,10084..10101,10102..10108)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10116..10132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10121..10143
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10140..10156
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10140..10156
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagcat ttgtactata tagattacta ttcttaacag
      361 tattactcct acaactatat aaactccata ttgtatatac tttatccaag aagcaaggtc
      421 aaaccaattg ccaaacacat cccagctatt taacttttgt aattcataca tgttcttctc
      481 ttgttgaatt tgtgcctcct ctaggagggc tgttatattt tcctccaaga agtcaacctt
      541 tcgctcccac tcttgccaag tctcattgtt ccactttggt gttagacttg catttggcca
      601 tggtacagta gtgtggcaga cttgtctaaa cgcacatccc caagcattca gctgcgcctg
      661 gtcctttaag tatttctcga tggcagtgac cctagtctgg aggttctttg ttccccagac
      721 ggtcagtcgc aacaattctt gttgtctctt gaccacgtcc aacagcagtt gctgttgctg
      781 cactatccca gccaataaag tccgggactg agcggtcagc gtcaacgacg ccgcgcccat
      841 tgcagaacct gccgttgcga gaaaacccaa gaaccctagc acaaagaccc ctcttttatt
      901 tcttgaggtg ccaccagtag tgtacctcct cacatttgtg ggggccaagc caattggagt
      961 gatctctact aatttataat ctcccaattc caatcgatac agttctgcca cctctgcact
     1021 catggtgata ttagtttggt ttccatcagt ccaatctatg tttgctatga gactggtcac
     1081 tgtggagata cacaattgtc cctcatatct cctcctctct cgggaacctg cagcaagaca
     1141 gcagttgttc tcccctgatg gcccttctca cttctcccgc tgtaaagcaa gggaaataag
     1201 tgctatgcag taaaatgtct gcatagtttg gtgttacatc tgtccaaaag ttctttgagt
     1261 accaggttac cctcactgca taagtactga gccacccttt ttctggtgtc aaatgccaat
     1321 acccttgtac ttctaaatgg cttccttcct gtagtgggaa gattactctg ctgcaggtcc
     1381 accatgccca tccgacctta aaatggggca catagcaaac cttttgtaga tctttagttt
     1441 tatatttcag atattttatg aggctatgcc acctctctag cctctccggt atcctccatg
     1501 tgggaactgc tatccatctc ttttcctcct ccataatctt tgataatttt agcctttctt
     1561 ctgggtacta ccttaatgtc tgtccctacc tttaagatga ctgctccttc ccctttccac
     1621 aatagctcac cgggtccctt ccacagttga tctctgcctt ctctgtaata gacccgaaaa
     1681 tttttaaatt ttgagttttt tgattgttga aattgtattt cttgttctgt agtgatcatg
     1741 ttaattaatc tttctgctgg agtcatatcc cctattcctc cccttctttt aaaattcatg
     1801 caatgaactg ccattaatac tatggtttct actgaatttg cttgttccct gattctatct
     1861 atttgatttt tcaggtggtg attcattgct tccactactc cctgactctg tggattgtat
     1921 ggtaccccaa aggtgtgctc tatccctgcc caccatgcaa ccatctttac ttcttgcgaa
     1981 gcaaagttag caccattatc tgtgtgtaga tgtgtaatag gccatctgcc tgccaatttt
     2041 aacagaaata gtgctgtctg tcttcctgtc tcttgtggaa ttacctctgc ttctatgaat
     2101 ccactagcta catgtactgc aactatgatt atttttccct ctagatgggt acaatccatt
     2161 tgccaagtcc ctagatctga atttacctgc ccatgtatag cttctccttt ctgatgacat
     2221 ttatcacagg tgtctactat ctgtctggcc actattctgg gtaatccaaa tttgaatacc
     2281 aattctttta cattactatg gtatttatca tgctcttctt gtgctggctc tatcttttcc
     2341 aagaagagaa cttgtctaat cccctgacta actaggtggt ctacttcttg gtttcctcct
     2401 atacctttgt gtgctggtac ccatgctaca taaatttctg actttttaat catttctttt
     2461 attatttggt taactagtct gctctctgat tctgtagggc atcctgttat tattcccata
     2521 acatattgtg aatctactat aatatttgcc tttggccctg agtctgttaa tgccatgagg
     2581 aatgcttcca attctgcctg ttgattagta gtctgttcta acacttttac tttgtctttg
     2641 cccctatctg tgatatatcc tgctttccct tcttttgact gtttattaca tgatccatct
     2701 gtataatagg tttcttctcc ctctataggg tccttcacta gattgaagac taatctcact
     2761 agtggtggtg ttgagataaa attccattct ggtatccagg ttacctgcca atagtctgtc
     2821 caccactgtt cccatacatc cttctcaact ggtaagtgga attttgggac ctgtccccag
     2881 atcactattg cttcctttcc tattttctgt attacatgtg ctaatagtct aactccattg
     2941 gtatgtgtat tctttatctt tgcaaatttt cctactttca gtattttgtc ttcttggtga
     3001 attttataag accactgatt gtcctgattc tttattaccg tggcttctaa tggcttgcct
     3061 tcttggtaat aacatccttc ttgttcctga ctgagaatta ttttattttc ctcatattct
     3121 gcttctgcca tctcagtcca ctgaacttcc tctgttagag tcatttttcc tctaattagc
     3181 ctacagagat gtttggtttt tatacctgga taaatttgag ctgcccaatt taatactcct
     3241 actaacttct gtatatcatt cactgtccag gtctctcttt gtggcaactc tatcttttgc
     3301 aacttccatt ttgttggcca caattcgtac cccatccatt gaaatggggg atctttttgg
     3361 aatttctctt ctggggtaga aaaccctatg ctatttaaga gttcctttaa ctgtaaaact
     3421 accctgtcat gttccaggtt tgtcctgtca ctagctatta agatgtcatc catatactgg
     3481 actaaggtca catctggatt tgccttcctg aagggttcta gcacatgtct catagtgtat
     3541 tggaagatgg ctggtgaccc cttccatcct tgaggcagaa ccttataaat gtatcgtttt
     3601 cctggctctg cattatttac tgatggtaaa gtaaaggcag tgtactgcct aaattcttca
     3661 tctagaggta tggagaaata tgcgtcacct atatccagta ctgtaatcct tttccttttt
     3721 gctagtcctg cagggtgtgg tattcctaat tggacttctg taaagtcctg agtgacccta
     3781 tttagttccc taaaatctat cagcattctc catttgttct tatctttttt ctttatagca
     3841 aatgtggggg tgttgtatgg attggtcggg ggagcttcct ccaactgacc atccttttcc
     3901 atcttttcac agatttctct taatgcaact atcttttctt ttgataatgg ccactgcttc
     3961 aattttggtc caacttttcc tggctttaag gcgactttta caggctctac tttagctatg
     4021 ggaagattta gagacatccc cagagctgtt agtaaatttc taccaaaaat gttaatcggg
     4081 gtgtcccctg tcatgattgt ccctctaatt cttttgccta aaacttctat ttctacattt
     4141 ttgtattctt tagtattaat aaaacctcct attcctccta ctatttttgg ggtataatgt
     4201 ggacctaact ctattcctgt tacaatagaa tcatcagccc ctgtatccag taatacttct
     4261 acaggctgtc cttcaatatg agcagtgact actggtctcc tccaaagaga gaattgaggt
     4321 gcagcaaatc ctctgtcatc tccttgtaag gcttctctct gctttctctc tgtttttctc
     4381 tctgctgctt gcccaactgc atgtagttct ttagcagatc cacagctggg tcctctgggg
     4441 gagcagttgg cgtcagcccc tgatgcactt gagccatggg gaaattgcgg ggcttctttc
     4501 cccatggacc aaggcctaaa aaacccgcct gtctgtctgg gcatttggcc ataacatggt
     4561 ccatttttcc acatttccag catccctgtc ttcttggggc tctgcattgc cttgcagagt
     4621 gtccctcttt cccacaattc caacacttaa ttggctttct tggtcccctc tgttgggctg
     4681 ctgcaaaagg gattggcact ggtgcgaggg cctctttcag ggcttctgcc attaatctag
     4741 ccttctgtcc tggcccccct actccttgac aagccgtcag catttcttct agggtgggat
     4801 tcacacccag ccccttcagc actagcttgc aatctgggtt agcattttga atcagcagtg
     4861 tttgagtcat ccaattcttt actgctgcat ctgtctgttc tgctcttaaa cttttgtaga
     4921 acctgtctac atagctctga aatggctctt ttggcccttg ttttacatct agaatgtttg
     4981 ttgggttata cattctgaca catttttgca accccagttg gatccatctc ctgtaaatgt
     5041 tgcctactgg tatggggttc tgttgtctgt acatccactg gatttgttca tctactgaac
     5101 tagttgttcc tgcaatatct gatcctgacg gctccctaag ctgtccttgt tgtggagctg
     5161 gttgtgggtg ctgcaagtcc caatctgcag cctcctcgtt tatgatgtct ctgataatct
     5221 gcatagccgc ttgatggtct cccacacaat ttaacatctg attaatgtca tagggggtgc
     5281 aaccttctga cagtgcctga aatcctggca ctacttctgc tccaaatttc ttttcctcta
     5341 tcaattttac ccaggcattt aatgttctcg ggcttaatgg caggtggaca tagttaccac
     5401 ctatttgttg tactgggtaa tttcctcctc tgccgctaga tggtgcagtt ggtctgcttg
     5461 tttttggcat aatttctgct gttcctgttt ccaccactag gtgtctctgt actatctgtt
     5521 ttgcttcctc agtgtgtttc actttctctt ctgcgtgaat gcaccagatg acgcagacag
     5581 tattataaag gctttttaaa ttttctgagc ctgttggcac taatggagct aagaccgaaa
     5641 gtattttttg acatccttct ttgttctcca acaggctttc tgctaatcca aatctatcta
     5701 attcatttgc tgcccatact acatgcttca acatgtactt tttctttccg ttgggtcgta
     5761 gcctaatttt ttctaattca tctgctttct tccctgacaa gacggagttt ctcgcgccca
     5821 tctcccactc tatcttatta tcccttcctg gataaaagac agctatttct tttttgtgtt
     5881 gcacttacct gcaaccggag gcctcttcct ctcccggtaa cccctcgaat tctttgccaa
     5941 aatgatgaga cagcacaata accagcacgt tgcccaggag ctgtaggaaa aagaagaagg
     6001 catgaacatg gttagcagag gctctagagc cgccggtcac acgccagaag ccgaaccccg
     6061 ccctgccccg tcccccccga aggcagccgt ccccccgcgg acagccccga ggctggagag
     6121 ggagaagggg acggcggcgc ggcgacgcac gaaggccctc cccgcccatt tccttcctgc
     6181 cggcgccgca ccgcttcgcc ccgcgcccgc tagagggggt gcggcggcgc ctcccagatt
     6241 tcggctccgc acagatttgg gacaaaggaa gtccctgcgc cctctcgcac gattaccata
     6301 aaaggcaatg gctgcggctc gccgcgcctc gacagccgcc ggcgctccgg gggccgccgc
     6361 gcccctcccc cgagccctcc ccggcccgag gcggccccgc cccgcccggc acccccacct
     6421 gccgccaccc cccgcccggc acggcgagcc ccgcgccacg ccccgtacgg agccccgcac
     6481 ccgaagccgg gccgtgctca gcaactcggg gaggggggtg cagggggggt tgcagcccga
     6541 ccgacgcgcc cacaccccct gctcaccccc ccacgcacac accccgcacg cagcctttgt
     6601 tcccctcgca gccccccccg caccgcgggg caccgccccc ggccgcgctc ccctcgcgca
     6661 cactgcggag cgcacaaagc cccgcgccgc gcccgcagcg ctcacagccg ccgggcagcg
     6721 cggagccgca cgcggcgctc cccacgcaca cacacacgca cgcacccccc gagccgctcc
     6781 ccccgcacaa agggccctcc cggagcccct caaggctttc acgcagccac agaaaagaaa
     6841 caagccgtca ttaaaccaag cgctaattac agcccggagg agaagggccg tcccgcccgc
     6901 tcacctgtgg gagtaacgcg gtcagtcaga gccggggcgg gcggcgcgag gcggcggcgg
     6961 agcggggcac ggggcgaagg cagcgcgcag cgactcccgc ccgccgcgcg cttcgctttt
     7021 tatagggccg ccgccgccgc cgcctcgcca taaaaggaaa ctttcggagc gcgccgctct
     7081 gattggctgc cgccgcacct ctccgcctcg ccccgccccg cccctcgccc cgccccgccc
     7141 cgcctggcgc gcsccccccc cccccccgcc cccatcgctg cacaaaataa ttaaaaaata
     7201 aataaataca aaattggggg tggggagggg ggggagatgg ggagagtgaa gcagaacgtg
     7261 gggctcacct cgaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
     7321 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
     7381 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
     7441 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
     7501 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
     7561 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
     7621 gattactatt aataactagt caataatcaa tgtcgaccca ggtggcactt ttcggggaaa
     7681 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7741 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7801 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7861 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7921 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7981 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8041 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8101 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8161 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8221 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8281 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8341 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8401 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8461 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8521 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8581 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8641 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8701 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8761 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8821 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8881 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8941 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     9001 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9061 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     9121 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9181 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9241 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9301 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9361 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9421 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9481 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     9541 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     9601 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcggat ccagacatga
     9661 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     9721 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     9781 ttagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     9841 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     9901 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     9961 tggttgctga ctaattgaga tgcggatccg ctggcacgac aggtttcccg actggaaagc
    10021 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
    10081 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
    10141 aggaaacagc tatgaccatg attacgccaa gcttgggctg caggtcgagg gatctccata
    10201 agagaagagg gacagctatg actgggagta gtcaggagag gaggaaaaat ctggctagta
    10261 aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa taacacaaaa caaaatat
//
