LOCUS       sequence-471209-        6017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation SIV-based lentiviral packaging plasmid; Contains SIV 
            Rev.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Benedetti L, Fan R, Weigel AV, Moore AS, Houlihan PR, Kittisopikul 
            M, Park G, Petruncio A, Hubbard PM, Pang S, Xu CS, Hess HF, Saalfeld
            S, Rangaraju V, Clapham DE, De Camilli P, Ryan TA, 
            Lippincott-Schwartz J
  TITLE     Periodic ER-plasma membrane junctions support long-range Ca(2+) 
            signal integration in dendrites.
  JOURNAL   Cell. 2025 Jan 23;188(2):484-500.e22. doi: 
            10.1016/j.cell.2024.11.029. Epub 2024 Dec 20.
  PUBMED    39708809
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.11.029"; journalName: "Cell"; date: 
            "2025-01-23- 23"; volume: "188"; issue: "2"; pages: "484-500.e22"
COMMENT     Sequence Label: SIV_Rev
FEATURES             Location/Qualifiers
     source          1..6017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     primer_bind     complement(1733..1755)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(2696..2972)
                     /label=chicken beta-actin promoter
     enhancer        2974..3353
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3384..3488
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3489..3557,3558..4349)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3707..3726)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4520..5108
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5009..5028
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5262..5279
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    5347..5481
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5371..5390)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5425..5444
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(5487..5682)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5501..5636
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    5741..5762
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5777..5782,5783..5800,5801..5807)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5815..5831
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5820..5842
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5839..5855
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5839..5855
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gacctggacc gcctcatgga aatagttcca
      361 cccatattgt aggtaggtca gttcagtcct gaggacttct cgaatccttc gtagggtcgc
      421 agagagcctc tggagtattg gttggaggat ctggtatact ctcgatagca aggttctgca
      481 gttgctgaat agccaagtca agaggcgtat cagttggcgg atcaggaaat gaatatattc
      541 tatctgccaa ggccaggagc tgttgccacc gccttctcca ccgtctcctt ctttgccttc
      601 tctggttggc agtgcctggt cctgttggat atgggtctac tggaaataag agggtgggga
      661 agagaacact ggcctatacc cctgccttaa cttagctagc atttgtacta tatagattac
      721 tattcttaac agtattactc ctacaactat ataaactcca tattgtatat actttatcca
      781 agaagcaagg tcaaaccaat tgccaaacac atcccagcta tttaactttt gtaattcata
      841 catgttcttc tcttgttgaa tttgtgcctc ctctaggagg gctgttatat tttcctccaa
      901 gaagtcaacc tttcgctccc actcttgcca agtctcattg ttccactttg gtgttagact
      961 tgcatttggc catggtacag tagtgtggca gacttgtcta aacgcacatc cccaagcatt
     1021 cagctgcgcc tggtccttta agtatttctc gatggcagtg accctagtct ggaggttctt
     1081 tgttccccag acggtcagtc gcaacaattc ttgttgtctc ttgaccacgt ccaacagcag
     1141 ttgctgttgc tgcactatcc cagccaataa agtccgggac tgagcggtca gcgtcaacga
     1201 cgccgcgccc attgcagaac ctgccgttgc gagaaaaccc aagaacccta gcacaaagac
     1261 ccctctttta tttcttgagg tgccaccagt agtgtacctc ctcacatttg tgggggccaa
     1321 gccaattgga gtgatctcta ctaatttata atctcccaat tccaatcgat acttacttgt
     1381 ttgatgcaga agatgtatta gccttagcct ttttcggagt tcttcttctc tttcgtgact
     1441 gctcataaca tatccccaag ccttttttaa gaaaacaaaa ctggcaatgg tagcaacact
     1501 ttttacaata gcatgtgtta tagcatgctt ctagagggcg gtatagctga gagaggattt
     1561 cttcccccag gttggccaat tctggagtgg atgcagccgc ctctgaagtg catgaagagt
     1621 gcccgctgga ggattctaat gagttctcct gctccctcaa gggtgtctcc atgtctatta
     1681 tagatatgat taccaagtgc agttagcaag cgaggagaat tctagagccg ccggtcacac
     1741 gccagaagcc gaaccccgcc ctgccccgtc ccccccgaag gcagccgtcc ccccgcggac
     1801 agccccgagg ctggagaggg agaaggggac ggcggcgcgg cgacgcacga aggccctccc
     1861 cgcccatttc cttcctgccg gcgccgcacc gcttcgcccc gcgcccgcta gagggggtgc
     1921 ggcggcgcct cccagatttc ggctccgcac agatttggga caaaggaagt ccctgcgccc
     1981 tctcgcacga ttaccataaa aggcaatggc tgcggctcgc cgcgcctcga cagccgccgg
     2041 cgctccgggg gccgccgcgc ccctcccccg agccctcccc ggcccgaggc ggccccgccc
     2101 cgcccggcac ccccacctgc cgccaccccc cgcccggcac ggcgagcccc gcgccacgcc
     2161 ccgtacggag ccccgcaccc gaagccgggc cgtgctcagc aactcgggga ggggggtgca
     2221 gggggggttg cagcccgacc gacgcgccca caccccctgc tcaccccccc acgcacacac
     2281 cccgcacgca gcctttgttc ccctcgcagc cccccccgca ccgcggggca ccgcccccgg
     2341 ccgcgctccc ctcgcgcaca ctgcggagcg cacaaagccc cgcgccgcgc ccgcagcgct
     2401 cacagccgcc gggcagcgcg gagccgcacg cggcgctccc cacgcacaca cacacgcacg
     2461 caccccccga gccgctcccc ccgcacaaag ggccctcccg gagcccctca aggctttcac
     2521 gcagccacag aaaagaaaca agccgtcatt aaaccaagcg ctaattacag cccggaggag
     2581 aagggccgtc ccgcccgctc acctgtggga gtaacgcggt cagtcagagc cggggcgggc
     2641 ggcgcgaggc ggcggcggag cggggcacgg ggcgaaggca gcgcgcagcg actcccgccc
     2701 gccgcgcgct tcgcttttta tagggccgcc gccgccgccg cctcgccata aaaggaaact
     2761 ttcggagcgc gccgctctga ttggctgccg ccgcacctct ccgcctcgcc ccgccccgcc
     2821 cctcgccccg ccccgccccg cctggcgcgc gccccccccc ccccccgccc ccatcgctgc
     2881 acaaaataat taaaaaataa ataaatacaa aattgggggt ggggaggggg gggagatggg
     2941 gagagtgaag cagaacgtgg ggctcacctc gaccatggta atagcgatga ctaatacgta
     3001 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     3061 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     3121 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     3181 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     3241 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg
     3301 aactaatgac cccgtaattg attactatta ataactagtc aataatcaat gtcgacccag
     3361 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     3421 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     3481 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     3541 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     3601 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     3661 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     3721 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     3781 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     3841 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     3901 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     3961 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4021 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4081 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     4141 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4201 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4261 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     4321 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     4381 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     4441 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4501 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4561 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4621 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     4681 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4741 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     4801 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     4861 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     4921 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     4981 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5041 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5101 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5161 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5221 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     5281 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     5341 gcagcggatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     5401 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     5461 ataagctgca ataaacaagt tagctttttg caaaagccta ggcctccaaa aaagcctcct
     5521 cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa
     5581 attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc
     5641 ggagttaggg gcgggactat ggttgctgac taattgagat gcggatccgc tggcacgaca
     5701 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     5761 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     5821 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cttgggctgc
     5881 aggtcgaggg atctccataa gagaagaggg acagctatga ctgggagtag tcaggagagg
     5941 aggaaaaatc tggctagtaa aacatgtaag gaaaatttta gggatgttaa agaaaaaaat
     6001 aacacaaaac aaaatat
//
