LOCUS       sequence-47121-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3030-02
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..761
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYIYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSRLSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
ORIGIN
        1 ggatccccgg gttaattaac ggtgacggtg ctggtttaat taacatgtct aaaggtgaag
       61 aattattcac tggtgttgtc ccaattttgg ttgaattaga tggtgatgtt aatggtcaca
      121 aattttctgt ctccggtgaa ggtgaaggtg atgctactta cggtaaattg accttaaaat
      181 ttatttgtac tactggtaaa ttgccagttc catggccaac cttagtcact actttaactt
      241 ggggtgttca atgtttttct agatacccag atcatatgaa acaacatgac tttttcaagt
      301 ctgccatgcc agaaggttat gttcaagaaa gaactatttt tttcaaagat gacggtaact
      361 acaagaccag agctgaagtc aagtttgaag gtgatacctt agttaataga atcgaattaa
      421 aaggtattga ttttaaagaa gatggtaaca ttttaggtca caaattggaa tacatttata
      481 actctcacaa tgtttacatc actgctgaca aacaaaagaa tggtatcaaa gctaacttca
      541 aaattagaca caacattgaa gatggttctg ttcaattagc tgaccattat caacaaaata
      601 ctccaattgg tgatggtcca gtcttgttac cagacaacca ttacttatcc actcaatcta
      661 gattatccaa agatccaaac gaaaagagag accacatggt cttgttagaa tttgttactg
      721 ctgctggtat tacccatggt attgatgaat tgtacaaata aggcgcgcca gatctgttta
      781 gcttgcctcg tcccc
//
