LOCUS       sequence-47123-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3020-02
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..762
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSRLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 cggatccccg ggttaattaa cggtgacggt gctggtttaa ttaacatgtc taaaggtgaa
       61 gaattattca ctggtgttgt cccaattttg gttgaattag atggtgatgt taatggtcac
      121 aaattttctg tctccggtga aggtgaaggt gatgctactt acggtaaatt gaccttaaaa
      181 tttatttgta ctactggtaa attgccagtt ccatggccaa ccttagtcac tactttaact
      241 tggggtgttc aatgtttttc tagataccca gatcatatga aacaacatga ctttttcaag
      301 tctgccatgc cagaaggtta tgttcaagaa agaactattt ttttcaaaga tgacggtaac
      361 tacaagacca gagctgaagt caagtttgaa ggtgatacct tagttaatag aatcgaatta
      421 aaaggtattg attttaaaga agatggtaac attttaggtc acaaattgga atacaactat
      481 aactctcaca atgtttacat catggctgac aaacaaaaga atggtatcaa agttaacttc
      541 aaaattagac acaacattga agatggttct gttcaattag ctgaccatta tcaacaaaat
      601 actccaattg gtgatggtcc agtcttgtta ccagacaacc attacttatc ctatcaatct
      661 agattatcca aagatccaaa cgaaaagaga gaccacatgg tcttgttaga atttgttact
      721 gctgctggta ttacccatgg tatggatgaa ttgtacaaat aaggcgcgcc agatctgttt
      781 agcttgcctc
//
