LOCUS       sequence-471233-       11621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the FlnA actin binding domain and Ig15 , a FRET construct 
            containing GFP-F40-RFP, the FlnA Ig 16 and Ig 24 domains..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11621)
  AUTHORS   Amiri S, Muresan C, Shang X, Huet-Calderwood C, Schwartz MA, 
            Calderwood DA, Murrell M
  TITLE     Intracellular tension sensor reveals mechanical anisotropy of the 
            actin cytoskeleton.
  JOURNAL   Nat Commun. 2023 Dec 4;14(1):8011. doi: 10.1038/s41467-023-43612-5.
  PUBMED    38049429
REFERENCE   2  (bases 1 to 11621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-12-4"; volume: "14(1):8011. doi"; pages: " 
            10.1038/s41467-023-43612-5"
COMMENT     Sequence Label: pLenti-CMV-puro_FlnaABD-15-TS-16-24flag
FEATURES             Location/Qualifiers
     source          1..11621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4159..4462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4463..4666
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4617..4637
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4663..4687
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4771..4795
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(6145..6147,6148..6150,6151..6663)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(6190..6211)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6451..6470)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6798..6819
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6955..7665
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             8413..8436
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(8444..8468)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8499..9087
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8552..8572)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8970..8981)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9101..9600
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9136..9159)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9533..9552
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9621..10220
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9621..9640)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10117..10137
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             10358..10591
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10663..10784
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10700..10719)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10754..10773
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10824..10959
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10979..10998)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10980..10998)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11008..11025)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11008..11024)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11017..11039)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11166..11594
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11253..11272)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11463..11484
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 ataagcttgg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     4201 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     4261 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     4321 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     4381 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     4441 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     4501 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     4561 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     4621 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     4681 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta
     4741 gtccagtgtg gtggaattct gcagatatca acaagtttgt acaaaaaagc aggcttcggt
     4801 accatgagta gctcccactc tcgggcgggc cagagcgcag caggcgcggc tccgggcggc
     4861 ggcgtcgaca cgcgggacgc cgagatgccg gccaccgaga aggacctggc ggaggacgcg
     4921 ccatggaaga agatccagca gaacactttc acgcgctggt gcaacgagca cctgaagtgc
     4981 gtgagcaagc gcatcgccaa cctgcagacg gacctgagcg acgggctgcg gcttatcgcg
     5041 ctgttggagg tgctcagcca gaagaagatg caccgcaagc acaaccagcg gcccactttc
     5101 cgccaaatgc agcttgagaa cgtgtcggtg gcactcgagt tcctggaccg cgagagcatc
     5161 aaactggtgt ccatcgacag caaggccatc gtggacggga acctgaagct gatcctgggc
     5221 ctcatctgga ccctgatcct gcactactcc atctccatgc ccatgtggga cgaggaggag
     5281 gatgaggagg ccaagaagca gacccccaag cagaggctcc tgggctggat ccagaacaag
     5341 ctgccgcagc tgcccatcac caacttcagc cgggactggc agagcggccg ggccctgggc
     5401 gccctggtgg acagctgtgc cccgggcctg tgtcctgact gggactcttg ggacgccagc
     5461 aagcccgtta ccaatgcgcg agaggccatg cagcaggcgg atgactggct gggcatcccc
     5521 caggtgatca cccccgagga gattgtggac cccaacgtgg acgagcactc tgtcatgacc
     5581 tacctgtccc agttccccaa ggccaagctg aagccagggg ctcccttgcg ccccaaatcc
     5641 gggatcgatg caaaggacgc cggggagggc ctgctggctg tccagatcac ggatcccgaa
     5701 ggcaagccga agaagacaca catccaagac aaccatgacg gcacgtatac agtggcctac
     5761 gtgccagacg tgacaggtcg ctacaccatc ctcatcaagt acggtggtga cgagatcccc
     5821 ttctccccgt accgcgtgcg tgccgtgccc accggggacg ccagcaagtg cactgtcaca
     5881 ggtgctggca tcggccccac cattcagatt ggggaggaga cggtgattac ggtcgacact
     5941 aaggcggcag gcaaaggcaa agtgacgtgc accgtgtgca cgcctgatgg ctcagaggtg
     6001 gatgtggacg tggtggagaa tgaggacggc actttcgaca tcttctacac ggccccccag
     6061 ccgggcaaat acgtcatctg tgtgcgcttt ggtggcgagc acgtgcccaa cagccccttc
     6121 caagtgacgg ctctggctgc tagcatggtg agcaagggcg aggagctgtt caccggggtg
     6181 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     6241 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     6301 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     6361 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     6421 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     6481 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     6541 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     6601 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     6661 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     6721 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     6781 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg ggggcccggt
     6841 ggtgcaggac caggtggtgc aggtccaggt ggtgcaggac caggtggtgc aggacctggt
     6901 ggtgcaggtc caggtggtgc aggtccaggt ggtgcaggtc caggtggtgc tcatatggtg
     6961 tctaagggcg aagagctgat taaggagaac atgcacatga agctgtacat ggagggcacc
     7021 gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc
     7081 cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg
     7141 gctaccagct tcatgtacgg cagcagaacc ttcatcaacc acacccaggg catccccgac
     7201 ttctttaagc agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac
     7261 gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac
     7321 gtcaagatca gaggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc
     7381 ggctgggagg ccaacaccga gatgctgtac cccgctgacg gcggcctgga aggcagaagc
     7441 gacatggccc tgaagctcgt gggcgggggc cacctgatct gcaacttcaa gaccacatac
     7501 agatccaaga aacccgctaa gaacctcaag atgcccggcg tctactatgt ggaccacaga
     7561 ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg agcagcacga ggtggctgtg
     7621 gccagatact gcgacctccc tagcaaactg gggcacaaac ttaatgctag cggggaccag
     7681 ccctcggtgc agccccctct acggtctcag cagctggccc cacagtacac ctacgcccag
     7741 ggcggccagc agacttgggc cccggagagg cccctggtgg gtgtcaatgg gctggatgtg
     7801 accagcctga ggccctttga ccttgtcatc cccttcacca tcaagaaggg cgagatcaca
     7861 ggggaggttc ggatgccctc aggcaaggtg gcgcagccca ccatcactga caacaaagac
     7921 ggcaccgtga ccgtgcggta tgcacccagc gaggctggcc tgcacgagat ggacatccgc
     7981 tatgacaaca tgcacatccc aggaagcccc ttgcagttct atgtggatta cgagctcagc
     8041 aaccacagcc tccacgagac atcatcagtg tttgtagact ctctgaccaa ggccacctgt
     8101 gccccccagc atggggcccc gggtcctggg cctgctgacg ccagcaaggt ggtggccaag
     8161 ggcctggggc tgagcaaggc ctacgtaggc cagaagagca gcttcacagt agactgcagc
     8221 aaagcaggca acaacatgct gctggtgggg gttcatggcc caaggacccc ctgcgaggag
     8281 atcctggtga agcacgtggg cagccggctc tacagcgtgt cctacctgct caaggacaag
     8341 ggggagtaca cactggtggt caaatggggg cacgagcaca tcccaggcag cccctaccgc
     8401 gttgtggtgc ccgactacaa agacgatgac gacaagtaat aagacccagc tttcttgtac
     8461 aaagtggttg atatccagca cagtggcggc cgctcgacaa tcaacctctg gattacaaaa
     8521 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     8581 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     8641 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     8701 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     8761 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     8821 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     8881 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     8941 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     9001 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     9061 ggatctccct ttgggccgcc tccccgcctg gaattctacc gggtagggga ggcgcttttc
     9121 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt
     9181 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
     9241 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
     9301 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
     9361 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
     9421 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc
     9481 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
     9541 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
     9601 acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     9661 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     9721 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     9781 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     9841 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     9901 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     9961 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
    10021 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
    10081 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
    10141 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
    10201 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg
    10261 cacgacccca tgcatctcga gggcccggta cctttaagac caatgactta caaggcagct
    10321 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctagc tcactcccaa
    10381 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
    10441 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
    10501 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
    10561 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
    10621 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
    10681 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    10741 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
    10801 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
    10861 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
    10921 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
    10981 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
    11041 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
    11101 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
    11161 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
    11221 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
    11281 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
    11341 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
    11401 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
    11461 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
    11521 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
    11581 atttaacgcg aattttaaca aaatattaac gcttacaatt t
//
