LOCUS       sequence-471234-        8888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MOV10 with N-terminal HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   Rheinemann L, Downhour DM, Davenport KA, McKeown AN, Sundquist WI, 
            Elde NC
  TITLE     Recurrent evolution of an inhibitor of ESCRT-dependent virus budding
            and LINE-1 retrotransposition in primates.
  JOURNAL   Curr Biol. 2022 Feb 25. pii: S0960-9822(22)00240-8. doi: 
            10.1016/j.cub.2022.02.018.
  PUBMED    35245459
REFERENCE   2  (bases 1 to 8888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2022 Feb 25. pii: S0960-9822(22)00240-8. doi: 
            10.1016/j.cub.2022.02.018."
COMMENT     Sequence Label: pHA-MOV10
FEATURES             Location/Qualifiers
     source          1..8888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    330..341
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(743..767)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(799..818)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(800..818)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(829..846)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(829..845)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(838..860)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    1250..1384
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1287..1306)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1341..1360
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1510..1965
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1597..1616)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1807..1828
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2082..2439
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2082..2273
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2105..2125)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2290..2425
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2490..3284
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2544..2563)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3154..3173
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3348..3396
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(3437..3456)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3556..3578
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        3702..3806
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3807..3875,3876..4667)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4025..4044)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4838..5426
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5327..5346
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5580..5597
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        complement(5671..5775)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5885..6188
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6189..6392
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6343..6363
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6389..6413
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6509..6535
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    6545..6569
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctgggcggg cctacctcga cagggctttc ccatcatctt tcacggcgta atgggcaaag
       61 atgagcgtga aggcaacagc ccatccttct tcaaccctga agaggctgcc acagtgactt
      121 cctacctgaa gctgctcctg gccccctcct ccaagaaggg caaagctcgc ctgagccctc
      181 gaagtgtggg cgtcatctcc ccgtaccgga aacaggtgga gaaaatccgt tactgcatca
      241 ccaaacttga cagggagctt cgaggactgg atgacatcaa ggacttgaag gtgggttcag
      301 tagaagaatt ccaaggccaa gaacgaagcg tcatcctcat ctccaccgtg cgaagcagcc
      361 agagctttgt gcagctggat ctggacttta atctgggttt ccttaagaac cccaagaggt
      421 tcaatgtagc tgtgacccgg gccaaggccc tgctcatcat cgtggggaac ccccttctcc
      481 tgggccatga ccctgactgg aaagtattcc tggagttctg taaagaaaac ggagggtata
      541 ccgggtgtcc cttccctgcc aaactggacc tgcaacaggg acagaattta ctgcaaggtc
      601 tgagcaagct cagcccctct acctcagggc cccacagcca tgactacctc ccccaggagc
      661 gggagggtga agggggcctg tctctgcaag tggagccaga gtggaggaat gagctctgag
      721 cggccgcact cgagatatct agacccagct ttcttgtaca aagtggttga tcgcgtgcat
      781 gcgacgtcat agctctctcc ctatagtgag tcgtattata agctaggcac tggccgtcgt
      841 tttacaacgt cgtgactggg aaaactgcta gcttgggatc tttgtgaagg aaccttactt
      901 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
      961 aaaattttta agtgtataat gtgttaaact agctgcatat gcttgctgct tgagagtttt
     1021 gcttactgag tatgatttat gaaaatatta tacacaggag ctagtgattc taattgtttg
     1081 tgtattttag attcacagtc ccaaggctca tttcaggccc ctcagtcctc acagtctgtt
     1141 catgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     1201 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     1261 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     1321 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     1381 gatcgatcct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattggct
     1441 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     1501 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     1561 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     1621 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     1681 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     1741 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     1801 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     1861 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     1921 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt
     1981 tctccttacg catctgtgcg gtatttcaca ccgcatacgc ggatctgcgc agcaccatgg
     2041 cctgaaataa cctctgaaag aggaacttgg ttaggtacct tctgaggcgg aaagaaccag
     2101 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     2161 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     2221 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     2281 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     2341 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     2401 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttgattc ttctgacaca
     2461 acagtctcga acttaaggct agagccacca tgattgaaca agatggattg cacgcaggtt
     2521 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     2581 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     2641 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     2701 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     2761 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     2821 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     2881 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     2941 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     3001 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     3061 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     3121 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     3181 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     3241 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     3301 cgaaatgacc gaccaagcga cgcccaacct gccatcacga tggccgcaat aaaatatctt
     3361 tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagcgata aggatccgcg
     3421 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     3481 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     3541 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     3601 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     3661 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     3721 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     3781 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     3841 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     3901 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     3961 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4021 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4081 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4141 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4201 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4261 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4321 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     4381 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     4441 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     4501 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     4561 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     4621 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     4681 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     4741 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     4801 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     4861 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     4921 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     4981 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5041 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5101 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5161 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5221 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5281 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5341 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5401 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5461 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     5521 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     5581 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     5641 gttggccgat tcattaatgc agagcttgca attcgcgctt tttcaatatt attgaagcat
     5701 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     5761 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     5821 tatcatgaca ttaacctata aaaataggcg tattacgagg ccctttcacg cattagatgc
     5881 atgtcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     5941 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     6001 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     6061 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     6121 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     6181 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     6241 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     6301 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     6361 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
     6421 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
     6481 ctctagccta ggccgcggta ccaccatgta tccgtatgat gtgccggatt atgcgtctag
     6541 atcaacaagt ttgtacaaaa aagcaggctc caccatggga accaattcag tcgacatgcc
     6601 cagtaagttc agctgccggc agctccggga ggcgggccag tgtttcgaga gtttcctggt
     6661 cgttcgggga ctggacatgg agacagatcg cgagcggctg cggaccattt ataaccgcga
     6721 cttcaagatc agctttggga cccccgcccc tggcttctcc tccatgctgt atggaatgaa
     6781 gattgcaaat ctggcctacg tcaccaagac tcgggtcagg ttcttcagac tcgaccgctg
     6841 ggccgacgtg cggttcccag aaaagaggag aatgaagctg gggtcagata tcagcaaaca
     6901 ccacaagtca ctgctagcca agatctttta tgacagggct gagtatcttc atgggaaaca
     6961 tggtgtggat gtggaagtcc aggggcccca tgaagcccga gatgggcagc tccttatccg
     7021 cctggatttg aaccgcaaag aggtgctgac cctgaggctt cggaatggcg gaacccagtc
     7081 tgttaccctc actcacctct tcccactctg ccggacaccc cagtttgctt tctacaatga
     7141 agaccaggag ttgccctgtc cactgggccc cggtgaatgc tatgaactcc atgtccattg
     7201 taagaccagc tttgtgggct acttcccagc cacagtgctc tgggagctgc tgggacctgg
     7261 ggagtcgggt tcagaaggag ccggcacatt ctacattgcc cgcttcttgg ctgccgtcgc
     7321 ccacagcccc ctggctgcac agctgaagcc catgactccc ttcaagcgga cccggatcac
     7381 cggaaaccct gtggtgacca atcggataga ggaaggagag agacctgacc gcgctaaggg
     7441 ctatgacctg gagttaagta tggcgctggg gacatactac ccacctcccc gcctcaggca
     7501 gctgctcccc atgcttcttc agggaacaag tatcttcact gcccctaagg agatcgcaga
     7561 gatcaaggcc cagctggaga cagccctgaa gtggaggaac tatgaggtga agctgcggct
     7621 gctgctgcac ctggaggaac tgcagatgga gcatgatatc cggcactatg acctggagtc
     7681 ggtgcccatg acctgggacc ctgtggacca gaaccccagg ctgctcacgc tggaggttcc
     7741 tggagtgact gagagccgcc cctcagtgct acggggcgac cacctgtttg cccttttgtc
     7801 ctcggagaca caccaggagg accccatcac atataagggc tttgtgcaca aggtggaatt
     7861 ggaccgtgtc aagctgagct tttccatgag cctcctgagc cgctttgtgg atgggctgac
     7921 cttcaaggtg aactttacct tcaaccgcca gccgctgcga gtccagcacc gtgccctgga
     7981 gctgacaggg cgctggctgc tgtggcccat gctctttcct gtggcacctc gggacgtccc
     8041 gctgctgccc tcagatgtga aactcaagct gtacgaccgg agtctggagt caaacccaga
     8101 gcagctgcag gccatgaggc acattgttac gggcaccacc cgtccagccc cctacatcat
     8161 ctttgggcct ccaggcaccg gcaagactgt cacgttagtg gaggcaatta agcaggtggt
     8221 gaagcacttg cccaaagccc acatcttggc ctgcgctcca tccaactcag gggctgacct
     8281 actctgtcaa aggctccggg tccaccttcc tagctccatc taccgcctcc tggcccccag
     8341 cagggacatc cgcatggtac ctgaggacat caagccctgc tgcaactggg acgcaaagaa
     8401 gggggagtat gtatttcccg ccaagaagaa gctgcaggaa taccgggtct taattaccac
     8461 cctcatcact gccggcaggt tggtctcggc ccagtttccc attgatcact tcacacacat
     8521 cttcatcgat gaggctggcc actgcatgga gcctgagagt ctggtagcta tagcagggct
     8581 gatggaagta aaggaaacag gtgatccagg agggcagctg gtgctggcag gagaccctcg
     8641 gcagctgggg cctgtgctgc gttccccact gacccagaag catggactgg gatactcact
     8701 gctggagcgg ctgctcacct acaactccct gtacaagaag ggccctgatg gctatgaccc
     8761 ccagttcata accaagctgc tccgcaacta caggtctcat cccaccatcc tggacattcc
     8821 taaccagctc tattatgaag gggagctgca ggcctgtgct gatgtcgtgg atcgagaacg
     8881 cttctgcc
//
