LOCUS       sequence-471235-       14870 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of voltage-gated calcium channel, CaV2.2, 
            N-terminally tagged with mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14870)
  AUTHORS   Benedetti L, Fan R, Weigel AV, Moore AS, Houlihan PR, Kittisopikul 
            M, Park G, Petruncio A, Hubbard PM, Pang S, Xu CS, Hess HF, Saalfeld
            S, Rangaraju V, Clapham DE, De Camilli P, Ryan TA, 
            Lippincott-Schwartz J
  TITLE     Periodic ER-plasma membrane junctions support long-range Ca(2+) 
            signal integration in dendrites.
  JOURNAL   Cell. 2025 Jan 23;188(2):484-500.e22. doi: 
            10.1016/j.cell.2024.11.029. Epub 2024 Dec 20.
  PUBMED    39708809
REFERENCE   2  (bases 1 to 14870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.11.029"; journalName: "Cell"; date: 
            "2025-01-23- 23"; volume: "188"; issue: "2"; pages: "484-500.e22"
COMMENT     Sequence Label: pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.2 WPRE
FEATURES             Location/Qualifiers
     source          1..14870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2694..3141
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3164..3173
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3170..3172,3173..3175,3176..3886)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3215..3236)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3476..3495)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3823..3844
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    10960..11548
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11013..11033)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11431..11442)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11891..11910)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    11956..12011
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(12010..12029)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(12372..12388)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12372..12388)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12385..12407)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12396..12412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12420..12426,12427..12444,12445..12450))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12465..12486
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12545..12740
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12591..12726
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    12746..12880
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12783..12802)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12837..12856
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12948..12965)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13119..13707)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13199..13218)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13878..14669,14670..14738))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14501..14520
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14739..14843)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgatcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacccttctt aacatggctg acaagaagga aactagatga gacagcaggg actttccaca
      421 aggggtgtta cggggaggta ctggggagga gccggtcggg aacacccact ttcttgatgt
      481 ataaatatca ctgcattttg ctatgtagtc agtcgctctg cggagaggct ggcagattga
      541 gccctgggag gttctctcca gcactagcag gtagagcctg ggtgttccct gctagactct
      601 caccagcact tggccggtgc tgggcagagt gactccacgc ttgcttgctt aaagccctct
      661 tcaataaagc tgccatttta gaagtaagct agtgtgtgtt cccatctctc ctagtcgccg
      721 cctggtcaac tcggtactcg gtaataagaa gaccctggtc tgttaggacc ctttctgctt
      781 tgggaaaccg aagcaggaaa atccctagca gattggcgcc cgaacaggga cttgaaggag
      841 agtgagagac tcctgagtac ggctgagtga aggcagtaag ggcggcagga accaaccacg
      901 acggagtgct cctataaagg cgcgggtcgg taccagacgg cgtgaggagc gggagaggaa
      961 gaggcctccg gttgcaggta agtgcaacac aaaaaagaaa tagctgtctt ttatccagga
     1021 agggataata agatagagtg ggagatgggc gcgagaaact ccgtcttgtc agggaagaaa
     1081 gcagatgaat tagaaaaaat taggctacga cccaacggaa agaaaaagta catgttgaag
     1141 catgtagtat gggcagcaaa tgaattagat agatttggat tagcagaaag cctgttggag
     1201 aacaaagaag gatgtcaaaa aatactttcg gtcttagctc cattagtgcc aacaggctca
     1261 gaaaatttaa aaagccttta taatactgtc tgcgtcatct ggtgcattca cgcagaagag
     1321 aaagtgaaac acactgagga agcaaaacag atagtacaga gacacagagg aagagcacca
     1381 tacaatccac agagtcaggg agtagtggaa gcaatgaatc accacctgaa aaatcaaata
     1441 gatagaatca gggaacaagc aaattcagta gaaaccatag tattaatggc agttcattgc
     1501 atgaatttta aaagaagggg aggaataggg gatatgactc cagcagaaag attaattaac
     1561 atgatcacta cagaacaaga aatacaattt caacaatcaa aaaactcaaa atttaaaaat
     1621 tttcgggtct attacagaga aggcagagat caactgtgga agggacccgg tgagctattg
     1681 tggaaagggg aaggagcagt catcttaaag gtagggacag acattaaggt agtacccaga
     1741 agaaaggcta aaattatcaa agattatgga ggaggaaaag agatggatag cagttcccac
     1801 atggaggata ccggagaggc tagagaggtg gcatagcctc atacaattcg attggaattg
     1861 ggagattata aattagtaga gatcactcca attggcttgg cccccacaaa tgtgaggagg
     1921 tacactactg gtggcacctc aagaaataaa agaggggtct ttgtgctagg gttcttgggt
     1981 tttctcgcaa cggcaggttc tgcaatgggc gcggcgtcgt tgacgctgac cgctcagtcc
     2041 cggactttat tggctgggat agtgcagcaa cagcaactgc tgttggacgt ggtcaagaga
     2101 caacaagaat tgttgcgact gaccgtctgg ggaacaaaga acctccagac tagggtcact
     2161 gccatcgaga aatacttaaa ggaccaggcg cagctgaatg cttggggatg tgcgtttaga
     2221 caagtctgcc acactactgt accatggcca aatgcaagtc taacaccaaa gtggaacaat
     2281 gagacttggc aagagtggga gcgaaaggtt gacttcttgg aggaaaatat aacagccctc
     2341 ctagaggagg cacaaattca acaagagaag aacatgtatg aattacaaaa gttaaatagc
     2401 tgggatgtgt ttggcaattg gtttgacctt gcttcttgga taaagtatat acaatatgga
     2461 gtttatatag ttgtaggagt aatactgtta agaatagtaa tctatatagt acaaatgcta
     2521 gctaagttaa ggcaggggta taggccagtg ttctcttccc caccctctta tttccagtag
     2581 acccatatcc aacaggacca ggcactgcca accagagaag gcaaagaagg agacggtgga
     2641 gaaggcggtg gttcgaacgc gccgtttcta gactgcagag ggccctgcgt atgagtgcaa
     2701 gtgggtttta ggaccaggat gaggcggggt gggggtgcct acctgacgac cgaccccgac
     2761 ccactggaca agcacccaac ccccattccc caaattgcgc atcccctatc agagaggggg
     2821 aggggaaaca ggatgcggcg aggcgcgtgc gcactgccag cttcagcacc gcggacagtg
     2881 ccttcgcccc cgcctggcgg cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg
     2941 tcactcgccg gtcccccgca aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc
     3001 gccggcccag ccggaccgca ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat
     3061 ctgcgctgcg gcgccggcga ctcagcgctg cctcagtctg cggtgggcag cggaggagtc
     3121 gtgtcgtgcc tgagagcgca gtcgagaaac ccgggaaccg gtcgccacca tggtgagcaa
     3181 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     3241 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     3301 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     3361 cttgacctac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     3421 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     3481 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     3541 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     3601 caactacaac agccacaagg tctatatcac cgccgacaag cagaagaacg gcatcaaggt
     3661 gaacttcaag acccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     3721 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     3781 ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     3841 cgtgaccgcc gccgggatca ctctcggcat ggacgagctc tacaagaagc ttgcatgcct
     3901 gcaggtcgac atggtccgct tcggggacga gctgggcggc cgctatgggg gcgcgggcgg
     3961 cgcggagcgg gcccggggcg gcggggccgg cggggctggc ggcccgggcc ccggggggct
     4021 gccgccgggc cagcgggtcc tctacaaaca gtcgatcgcg cagcgcgcgc ggaccatggc
     4081 gctgtacaac cccatcccgg tcaagcagaa ctgcttcacc gtcaaccgct cgctcttcgt
     4141 cttcagcgag gacaacgtcg tccgcaaata cgccaagcgc atcaccgagt ggcctccgtt
     4201 cgagtacatg atcctggcca ccatcatcgc caactgcatc gtgctggccc tggagcagca
     4261 cctccctgac ggggacaaga cgcctatgtc cgagcgcctg gatgacaccg agccctactt
     4321 cattgggatc ttctgcttcg aggcggggat caagatcctg gccctgggct ttgttctgca
     4381 caagggctcc tatctgcgga acggctggaa cgtcatggac ttcgtggtag tcctcacggg
     4441 gattcttgcc acagccggaa ccgacttcga cctgcgaacg ctgagggctg tgcgtgtgct
     4501 aaggcccctg aagctggtgt ctgggattcc gagcttgcag gtggtgctca agtccatcat
     4561 gaaggccatg gtcccgctgc tgcagatcgg gctgctgctc ttcttcgcca tcctcatgtt
     4621 tgccatcatc ggcctcgagt tctacatggg caagttccac aaggcctgct tccccaacag
     4681 cacagaccca gatcccgtgg gtgacttccc ctgtggcaag gaggccccgg cccggctctg
     4741 tgagggagac acggagtgcc gggagtactg ggccggaccc aactttggta tcaccaactt
     4801 tgacaacatc ctgtttgcca tcttgaccgt gttccagtgc atcaccatgg agggctggac
     4861 tgacatcctc tacaatacaa acgatgcagc cggcaacacg tggaactggc tctacttcat
     4921 ccctctcatc atcatcggct ccttcttcat gctcaacctg gtgctgggtg tgctctcagg
     4981 agagtttgct aaggagcgcg agagagtgga gaaccgccgc gccttcctga agctccggcg
     5041 gcagcagcag attgagcggg agctcaacgg gtacctggag tggatcttca aggctgagga
     5101 agtcatgctg gctgaggagg acaggaacgc agaggagaag tcccctctgg atgcagtgtt
     5161 gaagagagca gctgccaaga agagcagaag cgacctgatc caggccgagg agggggaggg
     5221 ccggctcaca ggcctctgtg ctcccggttc cccgttcgcc cgtgccagcc tgaaaagcgg
     5281 gaagacggag agctcgtcgt acttccggag gaaggagaag atgttccgct tcttcatccg
     5341 gcgcatggtg aaggcacaga gcttctactg gacggtgctg tgtgtggtgg cactgaacac
     5401 actgtgcgtg gccatggtgc actacaacca gccgcagcgg ctcaccacgg cattgtactt
     5461 tgcagagttt gttttcctgg gtctcttcct cacagagatg tccctgaaga tgtatggcct
     5521 ggggcccagg agctacttcc ggtcctcctt caactgtttt gactttgggg tcatcgtggg
     5581 cagtatcttt gaagtggtct gggctgctgt caagccaggg acctccttcg ggatcagtgt
     5641 tctgcgggct ctgcgcttac tgagaatctt caaggtcacc aaatactgga actccctacg
     5701 gaacctggtg gtgtctctgc tcaactccat gaagtccatc atcagcctcc tcttcctgct
     5761 cttcctgttc attgttgtct ttgctctgct ggggatgcag ctgtttggag gccagttcaa
     5821 ctttaaagac gagaccccaa cgaccaactt tgacaccttc cctgctgcta tcctcaccgt
     5881 cttccagatc ctgaccggag aggactggaa tgcggtcatg taccacggga tcgagtcgca
     5941 aggtggcgtc agcagaggaa tgttctcctc cttctacttc attgtcctca ctctgtttgg
     6001 gaactacacc ctgctgaatg tcttcctggc cattgctgtg gacaaccttg ccaatgctca
     6061 ggagctgacc aaggatgaag aggagatgga agaagcagcc aatcagaaac tcgccttgca
     6121 gaaggccaag gaagtggctg aagtcagccc catgtctgct gcgaacatct ccattgccgc
     6181 caggcagcag aactcggcca aagcgcgctc ggtgtgggag cagcgagcca gccagctgcg
     6241 gctgcagaac ctgagggcca gctgcgaggc gctgtacagc gagatggacc ccgaggagag
     6301 gctgcgctac gccaccgcgc gccacctgcg gcccgacgtg aagacgcacc tggaccggcc
     6361 gctggtggtt gagcccggcc gcgacgctcc gcggggcccc ccggggggga agtcccggcc
     6421 ggatggctcg gaggcccccg agggtgcgga tcccccgcgc aggcatcacc ggcaccgaga
     6481 caaggacaag gctcccgcca cggtcccctc ggcgggggaa caggacagag ccgaagctct
     6541 gagggcagag ggcggggagc tggggccccg cgaggagcgg gggcggccac gccgcagccg
     6601 cagcaaggag gccccgggcg ccccggaggt gcgcagcgat cgcggccgtg gcccgtgtcc
     6661 tgaaggtggt cggcgccacc accggcgcgg gtcaccggag gaggcagctg agagggagcc
     6721 ccgccgccac cgcgcgcacc ggcacgggcc cgacccgggc aaggagggcc cagcgtccgg
     6781 gaccaggggc gagcggcgcg cacgacaccg cacgggcccc cgggcgtgcc cccgggaggc
     6841 tgagagcagc gaggagcctg cgcggcggca ccgggctcgg cacaaggcgc cgcccaccca
     6901 ggagaccgcg gagaaggaca aggaggctgc cgagaagggg ggcgaggcca cggaggccga
     6961 gaaggacaag gaggctcgga accaccagcc caaggagctg ccctgtgacc tggaggccat
     7021 cggcatgctg ggagtgggcg ctgtgcatac gctgcccagc acctgtctgc agaaggtgga
     7081 ggagcagcca gaggatgcgg acaaccagcg gaacgtcacc cgcatgggta gtcagccccc
     7141 ggacaccagc accactgtgc acattcctgt gacgctgacc ggcccccccg gggagaccac
     7201 ggtagtgccc agtggaaacg tggacctgga aagtcaagca gaggggaaga aggaggtgga
     7261 aaccagcgat gtgatgagga gcggccctcg gcccattgtc ccgtacagtt ccatgttctg
     7321 tttaagccca accaacctgc tgcgccgctg ctgccactac attgtgacca tgcgatactt
     7381 cgagatggtc atcctcgtgg tcatcgccct gagcagcatc gccctggctg ccgaggaccc
     7441 cgtgagaaca gactctccca ggaataatgc tctgaagtac atggactaca tcttcacagg
     7501 cgtcttcacc ttcgagatgg tgataaagat gatcgatttg ggactgctgc tacaccctgg
     7561 agcctatttc cgggatctgt ggaacattct ggacttcatt gtggtcagtg gggccctggt
     7621 ggcatttgct ttctcaggat ccaaagggaa agacatcagc accatcaaat ccctgagagt
     7681 cctgcgtgtg ctgcggccac tcaagaccat caagcggctg cccaagctca aggccgtgtt
     7741 tgactgtgtg gtgaactcac tgaaaaacgt cctcaacatc ctgatcgtct acatgctctt
     7801 catgttcatc tttgccgtca ttgccgtgca gctcttcaaa gggaagttct tctactgcac
     7861 ggacgaatcc aaggagctgg agcgtgactg caggggtcag tatttggatt atgagaagga
     7921 ggaagtggaa gctcaaccga ggcagtggaa gaaatatgac ttccactatg acaacgtgct
     7981 ctgggctctg ctaacgctct tcacggtgtc cacaggggaa gggtggccca tggtgctgaa
     8041 acactctgtg gatgccacct acgaggagca gggcccgagc cccgggtacc gcatggagct
     8101 ctccatcttc tacgtggtct actttgtggt ctttcccttc ttctttgtca acatcttcgt
     8161 ggccttgatc atcatcacct tccaggagca gggggacaag gtgatgtccg agtgcagcct
     8221 ggagaagaac gagagggctt gcatcgactt tgccatcagc gccagacccc tgacgcggta
     8281 catgccccag aacaagcagt cgttccagta taagacgtgg acgttcgtgg tgtccccacc
     8341 ttttgagtac ttcatcatgg ctatgatcgc cctcaacact gtggtgctga tgatgaagtt
     8401 ctacgatgcg ccgtacgagt atgagctaat gctgaaatgc ctgaacatcg tgttcacgtc
     8461 catgttctcc atggagtgtg tgctgaagat catcgccttc ggggtgctga actatttcag
     8521 agatgcctgg aatgtctttg actttgtcac ggtgttggga agtattactg atattttggt
     8581 aacagagatt gcgaacaact tcatcaacct cagcttcctg cgcctgttcc gggccgcgcg
     8641 gctcatcaag ctgctgcgcc agggctacac catccgcatc ctgctttgga ccttcgtgca
     8701 gtccttcaag gcgctgccct acgtgtgtct cctcatcgcc atgctcttct tcatctacgc
     8761 catcattggc atgcaggttt ttgggaacat cgccctggac gatgacacca gcatcaacag
     8821 gcacaataac ttccggacgt ttctgcaggc cttgatgttg ctgttcagga gcgccacggg
     8881 ggaggcctgg cacgagataa tgctgtcgtg cctcagcagc cgggcctgcg acgagcactc
     8941 caatgccagc gagtgcggca gcgactttgc ctacttttac ttcgtctcct tcatcttcct
     9001 ctgctccttt ctgatgttga acctctttgt ggctgtgatc atggacaatt ttgagtacct
     9061 tacacgggac tcttccatct tagggcctca ccacctggat gagttcatcc gggtctgggc
     9121 tgaatacgac ccggccgcgt gtgggcgcat cagttacagt gacatgtttg agatgctgaa
     9181 gcacatgtcc ccacccctgg ggctggggaa gaagtgccct gctcgagtcg cgtacaagcg
     9241 cctggtccgc atgaacatgc ccatctccag tgaggacatg acggtgcact tcacatccac
     9301 gctgatggct ctcattcgga cagcgctgga catcaagctg gcaccagctg ggaccaagca
     9361 gcaccagtgc gacgcagagc tgaggaagga gatttcctgt gtgtgggcca atctgcccca
     9421 gaagaccctg gacttactgg taccacccca taagcctgac gagatgacgg tgggcaaagt
     9481 ctatgcagct ctgatgatct tcgacttcta caaacagaac aaaaccagca gagaccagac
     9541 tcagcaagcc cccggaggcc tgtcccagct gggccccgtg tccctgttcc acccgctgaa
     9601 ggccacgctg gagcagactc agccggcgct gcgtggagcc cgggctttcc tgcggcagaa
     9661 gagttctgcc tccctgagca acggcggggc cgtacaaacc caggagagcg gcatcaagga
     9721 atcggtttct tggggcactc agaggaccca ggatgtgctt tgcgaggcca gggcacccct
     9781 ggagcggggc cactcagctg agatcccagt cgggcagcct gggacactgg ctgtagatgt
     9841 ccagatgcag aacatgactc tgagtggccc tgacgccgag ccgcagccag ggctggagag
     9901 ccagggccga gccgcctcca tgccccgcct ggccgcagaa acacagcccg cgcctgacgc
     9961 cagccccatg aagcgctcca tctccacgct ggccccgcgc ccccacacgg cccggctcgg
    10021 cagcaccgcg ctggaccgcc cagcccccag ccaggccccg caccatcacc accaccgctg
    10081 ccaccgccgc agggacagga agcagcggtc cctggagaag ggtcccagcc tgtctgctga
    10141 cacagatggg gcccccgaca gcacggtggg gccaggactg cccacggggg agggcccccc
    10201 cggctgcagg cgggagcggg agcgcaggca ggagcggggc cggtcccagg agcggaggca
    10261 gccctcgtcc tcctcctcgg agaagcatcg cttctactcc tgtgaccgct tcgggggccg
    10321 cgaaccccca cagcccaagc cctccctcag tagtcacccc acgtcgccga cggccgggca
    10381 ggagccggga ccccacccgc aggggagtgg ttcggttcac gggagccccc tgctgtccac
    10441 atctggtgct agcaccccgg gccgggggcg gaggcagctc ccccagaccc ccttgacccc
    10501 ccgccccagc gtcacctaca agacggccaa ctcctcgcct gtccacttcg ccggggcacc
    10561 gagcggcctc cccgccttct cccccggccg gctcagccgg ggcctctcag aacacaacgc
    10621 cctgctgcag agggacccgc tcagccggcc cctggcgccc ggctcgcgca tcggctccga
    10681 cccctacctg gggcagcggc tggacagcga ggcgcccgcc cgtgccctgc ccgaggacgc
    10741 gcccgccttt gaggagaccg cggccagcaa ctcgggccgc tcctcccgga cctcgtacgt
    10801 gtcctcactg acctcgcagc ccccgccgct gcgccgcgtg cccaacggct accactgcac
    10861 tctggggctg ggcgggggcg gccgggcgcg gcgcggctgc caccaccccg accgcgaccg
    10921 ccggtgctag gttaacaagc ttcgaattct gcagtcgaca atcaacctct ggattacaaa
    10981 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
    11041 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
    11101 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
    11161 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
    11221 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
    11281 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
    11341 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
    11401 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
    11461 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
    11521 cggatctccc tttgggccgc ctccccgctg aggccggccg cggccgcatc gatgccgtat
    11581 acggtacccc taagaacaat gagttacaaa ttggcaatag acatgtctca ttttataaaa
    11641 gaaaaggggg gactggaagg gatttattat agtgcaagaa gacaagatct gcattttgct
    11701 atgtagtcag tcgctctgcg gagaggctgg cagattgagc cctgggaggt tctctccagc
    11761 actagcaggt agagcctggg tgttccctgc tagactctca ccagcacttg gccggtgctg
    11821 ggcagagtga ctccacgctt gcttgcttaa agccctcttc aataaagctt cagctgctcg
    11881 agctagcaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
    11941 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
    12001 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
    12061 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc
    12121 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
    12181 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
    12241 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
    12301 tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg
    12361 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
    12421 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
    12481 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
    12541 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
    12601 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
    12661 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
    12721 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata
    12781 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    12841 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc
    12901 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
    12961 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    13021 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    13081 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    13141 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    13201 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    13261 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    13321 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    13381 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    13441 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    13501 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    13561 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    13621 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    13681 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    13741 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    13801 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    13861 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    13921 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    13981 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    14041 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    14101 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    14161 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    14221 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    14281 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    14341 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    14401 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    14461 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    14521 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    14581 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    14641 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    14701 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    14761 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    14821 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg
//
