LOCUS       sequence-471240-       10275 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac-integrated plasmid containing hygromycin resistance gene, 
            ZNF35/ZNF35-driven BFP, and ZNF35/ZNF35-NZF transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10275)
  AUTHORS   Wolfsberg E, Paul J-P, Tycko J, Chen B, Bassik MC, Bintu L, Alizadeh
            AA, Gao XJ
  TITLE     Machine-Guided Dual-Objective Protein Engineering for Deimmunization
            and Therapeutic Functions
  JOURNAL   bioRxiv 2025.02.24.639918
REFERENCE   2  (bases 1 to 10275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.02.24.639918"
COMMENT     Sequence Label: EW371 (PB) (ZNF35/ZNF35tar-6bp spacers)x4-H2B-...
FEATURES             Location/Qualifiers
     source          1..10275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(170..961,962..1030))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     793..812
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1031..1135)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1161..1616)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1298..1319)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1510..1529
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1743..1765
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1757..1774
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1758..1774
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1904..1966
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     5'UTR           2646..2688
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             2737..3114
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             3133..3840
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     polyA_signal    3874..4081
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4114..4134)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4119..4448
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4299..4434
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4361..4380
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4497..5522
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4497..5522
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(5582..5602)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5644..5765
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5681..5700)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5735..5754
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        6260..6639
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6640..6843
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6794..6814
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    6845..6863
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6866..6884
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6894..6918
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             8189..8218
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    8280..8487
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8759..8793
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9284..9300)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9284..9300)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9297..9319)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9308..9324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9332..9338,9339..9356,9357..9362))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9377..9398
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9515..9532)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9686..10274)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9766..9785)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
       61 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
      121 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
      181 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
      241 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
      301 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
      361 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
      421 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
      481 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
      541 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
      601 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
      661 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
      721 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
      781 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
      841 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
      901 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      961 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     1021 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     1081 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     1141 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     1201 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     1261 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     1321 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     1381 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     1441 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     1501 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     1561 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
     1621 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     1681 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     1741 ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt
     1801 tgaacccgtg gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca
     1861 gatgaagatg ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca
     1921 tattgtgacg tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct
     1981 gtatatcgag gtttatttat taatttgaat agatattaag ttttattata tttacactta
     2041 catactaata ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac
     2101 aaaaactcaa aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca
     2161 aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg
     2221 ggctccgctc cggtccggcg ctccccccgc atcccagagc cggcagcgtg cggggacagc
     2281 ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg
     2341 agcctgcaga cacctggggg gatacgggga aaaggcctcc acggccacaa ttgacgattg
     2401 agtaacactg tatctagtct acctacaggg ctcatatata aagagatata taaagagctc
     2461 ttcatatata aagagatata taaagaggag gtcatatata aagagatata taaagagcag
     2521 aacatatata aagagatata taaagagtat cagtctagag ggtatataat ggatcccatc
     2581 gcgtctcagc ctcactttga gctcctccac acgaattccc cgataccgtc gattcaagga
     2641 gcttgcttgt tctttttgca gaagctcaga ataaacgctc aactttggca gatctaccaa
     2701 gcttggtacc gagctcggat ccaactcgag gccaccatgc cagagccagc gaagtctgct
     2761 cccgccccga aaaagggctc caagaaggcg gtgactaagg cgcagaagaa aggcggcaag
     2821 aagcgcaagc gcagccgcaa ggagagctat tccatctatg tgtacaaggt tctgaagcag
     2881 gtccaccctg acaccggcat ttcgtccaag gccatgggca tcatgaattc gtttgtgaac
     2941 gacattttcg agcgcatcgc aggtgaggct tcccgcctgg cgcattacaa caagcgctcg
     3001 accatcacct ccagggagat ccagacggcc gtgcgcctgc tgctgcctgg ggagttggcc
     3061 aagcacgccg tgtccgaggg tactaaggcc atcaccaagt acaccagcgc taaggatccc
     3121 cgggtaccgg tcgtgtctaa gggcgaagag ctgattaagg agaacatgca catgaagctg
     3181 tacatggagg gcaccgtgga caaccatcac ttcaagtgca catccgaggg cgaaggcaag
     3241 ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc tctccccttc
     3301 gccttcgaca tcctggctac tagcttcctc tacggcagca agaccttcat caaccacacc
     3361 cagggcatcc ccgacttctt caagcagtcc ttccctgagg gcttcacatg ggagagagtc
     3421 accacatacg aggacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc
     3481 tgcctcatct acaacgtcaa gatcagaggg gtgaacttca catccaacgg ccctgtgatg
     3541 cagaagaaaa cactcggctg ggaggccttc accgaaacgc tgtaccccgc tgacggcggc
     3601 ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac
     3661 gccaagacca catatagatc caagaaaccc gctaagaacc tcaagatgcc tggcgtctac
     3721 tatgtggact acagactgga aagaatcaag gaggccaaca acgaaaccta cgtcgagcag
     3781 cacgaggtgg cagtggccag atactgcgac ctccctagca aactggggca caagcttaat
     3841 tagctagcga attcgaattt aaatcggcct cgactgtgcc ttctagttgc cagccatctg
     3901 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     3961 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     4021 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     4081 acgcggccgc aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     4141 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4201 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4261 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4321 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4381 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4441 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgatga
     4501 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     4561 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     4621 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     4681 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     4741 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     4801 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     4861 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     4921 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     4981 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     5041 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     5101 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     5161 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     5221 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     5281 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     5341 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     5401 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     5461 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
     5521 aggtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     5581 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgcgttaa
     5641 ctaaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     5701 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     5761 tcttatcatg tctggaattg actcaaatga tgtcaattag tctatcagaa gctcatctgg
     5821 tctcccttcc gggggacaag acatccctgt ttaatattta aacagcagtg ttcccaaact
     5881 gggttcttat atcccttgct ctggtcaacc aggttgcagg gtttcctgtc ctcacaggaa
     5941 cgaagtccct aaagaaacag tggcagccag gtttagcccc ggaattgact ggattccttt
     6001 tttagggccc attggtatgg ctttttcccc gtatcccccc aggtgtctgc aggctcaaag
     6061 agcagcgaga agcgttcaga ggaaagcgat cccgtgccac cttccccgtg cccgggctgt
     6121 ccccgcacgc tgccggctcg gggatgcggg gggagcgccg gaccggagcg gagccccggg
     6181 cggctcgctg ctgcccccta gcgggggagg gacgtaatta catccctggg ggctttgggg
     6241 gggggctgtc cctgatgttg acattgatta ttgactagtt attaatagta atcaattacg
     6301 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     6361 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     6421 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     6481 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     6541 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     6601 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     6661 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     6721 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     6781 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     6841 gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg acgagctcgt
     6901 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     6961 caccgggacc gatccagcct ccggactcta gcgtttaaac ttaagcttcg ccaccatgta
     7021 cgagtgtaat gagtgcggta agaccttcac acgatcttct aaccttattg tgcatcaacg
     7081 aatccataca ggcgagaagc cctttgcctg caatgactgt gggaaggctt ttactcagag
     7141 tgccaacttg atcgtgcatc agagaagcca tacgggtgag aagccctatg aatgcaagga
     7201 atgcggaaag gccttctcct gcttcagcca cctcattgta caccagcgca tacacacggc
     7261 tgagaagcca tacgattgct ctgaatgtgg aaaggccttt agtcagctgt cttgcttgat
     7321 tgtgcatcag agaattcaca cgggcgaacg cccctacgag tgtaatgagt gcggtaagac
     7381 cttcacacga tcttctaacc ttattgtgca tcaacgaatc catacaggcg agaagccctt
     7441 tgcctgcaat gactgtggga aggcttttac tcagagtgcc aacttgatcg tgcatcagag
     7501 aagccatacg ggtgagaagc cctatgaatg caaggaatgc ggaaaggcct tctcctgctt
     7561 cagccacctc attgtacacc agcgcataca cacggctgag aagccatacg attgctctga
     7621 atgtggaaag gcctttagtc agctgtcttg cttgattgtg catcagagaa ttcacgctgc
     7681 tgcatgggag ctaggaatgt ctggcggatc tgagggccag agcgacgaga gagccctgct
     7741 ggaccagctg cacaccctgc tgagcaacac cgacgccacc ggcctggagg agatcgacag
     7801 agccctgggc atccccgagc tggtgaacca gggccaggcc ctggagccca agcaggacgg
     7861 aggctgggga tctatggccg aggaattcgt gaccctgaag gacgtgggca tggacttcac
     7921 cctgggcgac tgggagcagc tgggcctgga gcagggcgac accttctggg acaccgccct
     7981 ggacaactgc caggacctgt tcctgctgga cccccccggc ggaggagacg gccttggttt
     8041 gggacacgag aagttcccca gcgacctgga cctggacatg ttcaacggca gcctggagtg
     8101 cgacatggag agcatcatca gaagcgagct gatggacgcc gacggcctgg acttcaactt
     8161 cgacagctag gccgctcgag gagggcccga acaaaaactc atctcagaag aggatctgaa
     8221 tagcgccgtc gaccatcatc ataatcatca ttgagtttaa acccgctgat cagcctcgac
     8281 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     8341 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     8401 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     8461 ggaagacaat agcaggcatg ctggggaatc tataacaaga aaatatatat ataataagtt
     8521 atcacgtaag tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt
     8581 cacgtaaaag ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt
     8641 gacacttacc gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc
     8701 taaatgcaca gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca
     8761 tgcgtcaatt ttacgcagac tatctttcta gggttaatct agctgcatca ggatcatatc
     8821 gtcgggtctt ttttccggct cagtcatcgc ccaagctggc gctatctggg catcggggag
     8881 gaagaagccc gtgccttttc ccgcgaggtt gaagcggcat ggaaagagtt tgccgaggat
     8941 gactgctgct gcattgacgt tgagcgaaaa cgcacgttta ccatgatgat tcgggaaggt
     9001 gtggccatgc acgcctttaa cggtgaactg ttcgttcagg ccacctggga taccagttcg
     9061 tcgcggcttt tccggacaca gttccggatg gtcagcccga agcgcatcag caacccgaac
     9121 aataccggcg acagccggaa ctgccgtgcc ggtgtgcaga ttaatgacag cggtgcggcg
     9181 ctgggatatt acgtcagcga ggacgggtat cctggctgga tgccgcagaa atggacatgg
     9241 ataccccgtg agttacccgg cgggcgcgct tggcgtaatc atggtcatag ctgtttcctg
     9301 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     9361 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     9421 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9481 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9541 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9601 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9661 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9721 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9781 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9841 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9901 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9961 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10021 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10081 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    10141 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10201 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10261 aaaaaggatc tcaag
//
