LOCUS       sequence-471249-       14408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of voltage-gated calcium channel, CaV1.2, 
            N-terminally tagged with mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14408)
  AUTHORS   Benedetti L, Fan R, Weigel AV, Moore AS, Houlihan PR, Kittisopikul 
            M, Park G, Petruncio A, Hubbard PM, Pang S, Xu CS, Hess HF, Saalfeld
            S, Rangaraju V, Clapham DE, De Camilli P, Ryan TA, 
            Lippincott-Schwartz J
  TITLE     Periodic ER-plasma membrane junctions support long-range Ca(2+) 
            signal integration in dendrites.
  JOURNAL   Cell. 2025 Jan 23;188(2):484-500.e22. doi: 
            10.1016/j.cell.2024.11.029. Epub 2024 Dec 20.
  PUBMED    39708809
REFERENCE   2  (bases 1 to 14408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.11.029"; journalName: "Cell"; date: 
            "2025-01-23- 23"; volume: "188"; issue: "2"; pages: "484-500.e22"
COMMENT     Sequence Label: pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV1.2 WPRE
FEATURES             Location/Qualifiers
     source          1..14408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2694..3141
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3164..3173
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3170..3172,3173..3175,3176..3886)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3215..3236)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3476..3495)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3823..3844
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(4871..4885)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     misc_feature    10498..11086
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10551..10571)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10969..10980)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11429..11448)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    11494..11549
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(11548..11567)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(11910..11926)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11910..11926)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11923..11945)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11934..11950
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11958..11964,11965..11982,11983..11988))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12003..12024
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12083..12278
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12129..12264
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    12284..12418
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12321..12340)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12375..12394
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12486..12503)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12657..13245)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12737..12756)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13416..14207,14208..14276))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14039..14058
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14277..14381)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgatcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacccttctt aacatggctg acaagaagga aactagatga gacagcaggg actttccaca
      421 aggggtgtta cggggaggta ctggggagga gccggtcggg aacacccact ttcttgatgt
      481 ataaatatca ctgcattttg ctatgtagtc agtcgctctg cggagaggct ggcagattga
      541 gccctgggag gttctctcca gcactagcag gtagagcctg ggtgttccct gctagactct
      601 caccagcact tggccggtgc tgggcagagt gactccacgc ttgcttgctt aaagccctct
      661 tcaataaagc tgccatttta gaagtaagct agtgtgtgtt cccatctctc ctagtcgccg
      721 cctggtcaac tcggtactcg gtaataagaa gaccctggtc tgttaggacc ctttctgctt
      781 tgggaaaccg aagcaggaaa atccctagca gattggcgcc cgaacaggga cttgaaggag
      841 agtgagagac tcctgagtac ggctgagtga aggcagtaag ggcggcagga accaaccacg
      901 acggagtgct cctataaagg cgcgggtcgg taccagacgg cgtgaggagc gggagaggaa
      961 gaggcctccg gttgcaggta agtgcaacac aaaaaagaaa tagctgtctt ttatccagga
     1021 agggataata agatagagtg ggagatgggc gcgagaaact ccgtcttgtc agggaagaaa
     1081 gcagatgaat tagaaaaaat taggctacga cccaacggaa agaaaaagta catgttgaag
     1141 catgtagtat gggcagcaaa tgaattagat agatttggat tagcagaaag cctgttggag
     1201 aacaaagaag gatgtcaaaa aatactttcg gtcttagctc cattagtgcc aacaggctca
     1261 gaaaatttaa aaagccttta taatactgtc tgcgtcatct ggtgcattca cgcagaagag
     1321 aaagtgaaac acactgagga agcaaaacag atagtacaga gacacagagg aagagcacca
     1381 tacaatccac agagtcaggg agtagtggaa gcaatgaatc accacctgaa aaatcaaata
     1441 gatagaatca gggaacaagc aaattcagta gaaaccatag tattaatggc agttcattgc
     1501 atgaatttta aaagaagggg aggaataggg gatatgactc cagcagaaag attaattaac
     1561 atgatcacta cagaacaaga aatacaattt caacaatcaa aaaactcaaa atttaaaaat
     1621 tttcgggtct attacagaga aggcagagat caactgtgga agggacccgg tgagctattg
     1681 tggaaagggg aaggagcagt catcttaaag gtagggacag acattaaggt agtacccaga
     1741 agaaaggcta aaattatcaa agattatgga ggaggaaaag agatggatag cagttcccac
     1801 atggaggata ccggagaggc tagagaggtg gcatagcctc atacaattcg attggaattg
     1861 ggagattata aattagtaga gatcactcca attggcttgg cccccacaaa tgtgaggagg
     1921 tacactactg gtggcacctc aagaaataaa agaggggtct ttgtgctagg gttcttgggt
     1981 tttctcgcaa cggcaggttc tgcaatgggc gcggcgtcgt tgacgctgac cgctcagtcc
     2041 cggactttat tggctgggat agtgcagcaa cagcaactgc tgttggacgt ggtcaagaga
     2101 caacaagaat tgttgcgact gaccgtctgg ggaacaaaga acctccagac tagggtcact
     2161 gccatcgaga aatacttaaa ggaccaggcg cagctgaatg cttggggatg tgcgtttaga
     2221 caagtctgcc acactactgt accatggcca aatgcaagtc taacaccaaa gtggaacaat
     2281 gagacttggc aagagtggga gcgaaaggtt gacttcttgg aggaaaatat aacagccctc
     2341 ctagaggagg cacaaattca acaagagaag aacatgtatg aattacaaaa gttaaatagc
     2401 tgggatgtgt ttggcaattg gtttgacctt gcttcttgga taaagtatat acaatatgga
     2461 gtttatatag ttgtaggagt aatactgtta agaatagtaa tctatatagt acaaatgcta
     2521 gctaagttaa ggcaggggta taggccagtg ttctcttccc caccctctta tttccagtag
     2581 acccatatcc aacaggacca ggcactgcca accagagaag gcaaagaagg agacggtgga
     2641 gaaggcggtg gttcgaacgc gccgtttcta gactgcagag ggccctgcgt atgagtgcaa
     2701 gtgggtttta ggaccaggat gaggcggggt gggggtgcct acctgacgac cgaccccgac
     2761 ccactggaca agcacccaac ccccattccc caaattgcgc atcccctatc agagaggggg
     2821 aggggaaaca ggatgcggcg aggcgcgtgc gcactgccag cttcagcacc gcggacagtg
     2881 ccttcgcccc cgcctggcgg cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg
     2941 tcactcgccg gtcccccgca aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc
     3001 gccggcccag ccggaccgca ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat
     3061 ctgcgctgcg gcgccggcga ctcagcgctg cctcagtctg cggtgggcag cggaggagtc
     3121 gtgtcgtgcc tgagagcgca gtcgagaaac ccgggaaccg gtcgccacca tggtgagcaa
     3181 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     3241 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     3301 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     3361 cttgacctac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     3421 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     3481 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     3541 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     3601 caactacaac agccacaagg tctatatcac cgccgacaag cagaagaacg gcatcaaggt
     3661 gaacttcaag acccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     3721 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     3781 ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     3841 cgtgaccgcc gccgggatca ctctcggcat ggacgagctc tacaagtccg gactcagatc
     3901 tcgagctcaa gcttcgaatt ctgcagtcga cggtatcgat aagctttgca gaatgcttcg
     3961 agcccttgtt cagccagcta cgcccgcata ccagccgctg cctagccacc tgtctgctga
     4021 aacggagagt acatgtaaag gtactgtggt gcatgaagct caactcaacc atttctacat
     4081 ctctcctgga ggttccaact atgggagccc acgcccagct catgccaaca tgaatgccaa
     4141 cgcagctgcg gggctcgccc ctgagcacat ccccacccca ggggcagccc tgtcctggca
     4201 ggcagccatc gatgcggccc ggcaggccaa gctgatgggc agtgctggca acgcgactat
     4261 ctccaccgtc agctccacgc agcggaagcg gcagcagtat gggaagccca agaagcaggg
     4321 cagcaccact gccactcgcc cgccccgtgc cctgctctgc ctcaccctga agaaccccat
     4381 ccggagggcg tgcataagca tcgtcgagtg gaaaccattt gaaataatta ttttactgac
     4441 tatttttgcc aattgtgtgg ccttagcaat ctatattccc tttccagaag atgactccaa
     4501 tgccaccaat tccaacctgg aacgagtgga atatctcttt ctcataattt ttactgtgga
     4561 agcattttta aaagtaatag cctatggact tctgtttcac cccaacgctt acctccgcaa
     4621 tggctggaat ttactagact ttataattgt ggttgtaggg ctttttagtg caattttaga
     4681 acaagcaacc aaagcagacg gggccaatgc cctaggaggg aaaggggctg gattcgacgt
     4741 gaaggcgctg agggctttcc gcgtgctgcg ccccctgcgg ctggtgtctg gagtcccgag
     4801 tctccaggtg gtcctgaact ccatcatcaa ggccatggtc cctctgctgc acattgccct
     4861 gctagtgctg tttgtcatca tcatctatgc catcatcggc ctggagctct tcatggggaa
     4921 gatgcacaag acatgctaca accaggaggg tgtagcagat gtcccagcag aagatgatcc
     4981 ttccccttgt gctctggaga cgggccacgg gcggcagtgc cagaacggca ccgtgtgcaa
     5041 gcctgggtgg gatggaccca agcacggcat caccaacttt gacaattttg ctttcgccat
     5101 gttgacggtg ttccagtgta tcaccatgga gggctggacc gacgtgctgt actggatgca
     5161 ggacgctatg ggctatgagc taccctgggt gtattttgtc agtctggtca tctttggatc
     5221 ctttttcgtt ctaaatctgg ttctcggtgt gttgagcgga gagttttcca aagagaggga
     5281 gaaggccaaa gctcggggag atttccagaa gttgcgggag aagcagcagc tggaagagga
     5341 cctcaaaggc tacctggact ggatcactca ggcagaagac atcgaccctg agaatgagga
     5401 tgaaggcatg gatgaggaga aaccccgaaa catgagcatg cccacaagtg agaccgaatc
     5461 tgtcaacact gaaaacgtgg ctggaggtga catcgaagga gaaaactgcg gggccaggct
     5521 ggcccaccgg atctccaagt cgaaattcag ccgctactgg cgccggtgga ataggttctg
     5581 caggagaaag tgccgcgcag cggtcaagtc gaacgtcttc tactggctgg tgatcttcct
     5641 ggtcttcctg aacacgctca ccattgcctc tgagcactac aaccagcccc actggctcac
     5701 ggaggtccaa gacacggcca ataaggctct actggccctg ttcactgccg agatgctgct
     5761 gaagatgtac agcctgggcc tgcaggccta tttcgtgtcc ctcttcaacc gcttcgactg
     5821 cttcattgtg tgcgggggca tcctggagac catcctggtg gagaccaagg tcatgtcccc
     5881 cctgggcatc tctgtgctga gatgcgtgcg gctcctgaga atattcaaaa ttacaaggta
     5941 ctggaactcc ttgagcaacc tggtggcctc cctgctgaac tcggtgcgct ccatcgcctc
     6001 cctgctcctg ctcctcttcc tcttcatcat catcttctcc ctgctgggga tgcagctgtt
     6061 tggaggcaag ttcaacttcg atgagatgca gacccggagg agcacgttcg acaatttccc
     6121 gcagtccctg ctcaccgtgt ttcagatcct gaccggggag gactggaatt cggtgatgta
     6181 tgatgggatc atggcttatg gcggcccctc ttttccaggg atgttagtct gtatttactt
     6241 catcatcctc ttcatctgtg gaaattatat cctactgaat gtgttcttgg ccattgctgt
     6301 ggacaacctg gctgatgctg agagccttac ttctgcccaa aaggaagagg aagaagagaa
     6361 ggagagaaag aagctggcca ggactgccag cccggagaag aaacaagagg tggtagggaa
     6421 gccggccctg gaggaggcca aggaggagaa aattgagctg aaatccatta cagctgatgg
     6481 agagtccccg cctaccacca agatcaacat ggatgacctc cagcccaatg agagtgagga
     6541 taagagtccc taccccaacc cggaaaccac aggagaagag gatgaggagg agcctgagat
     6601 gcctgtcggc ccccgccctc ggccactctc cgagctgcac cttaaggaga aggccgtgcc
     6661 tatgccagaa gccagtgcgt ttttcatctt cagccccaac aacaggttcc gcctccagtg
     6721 tcaccgtatc gtcaacgaca cgatcttcac caacctgatc ctcttcttca ttctgctcag
     6781 cagcatttcc ctggctgccg aggaccctgt gcagcacacc tccttcagga atcacattct
     6841 gttttatttt gacattgttt ttactaccat tttcaccatt gaaattgctc tcaagatgac
     6901 tgcgtatggg gccttcctgc acaagggctc tttctgcagg aactacttca acatcctgga
     6961 cctgctggtg gtcagcgtgt ccctcatctc cttcggcatc cagtccagcg cgatcaatgt
     7021 cgtgaagatc ttgcgagtgc tgcgagtgct caggccactg cgggccatca acagggccaa
     7081 ggggctaaag cacgtggttc agtgtgtgtt cgtggccatc cggaccattg ggaacatcgt
     7141 gattgtcacc acgctgctgc agttcatgtt cgcctgcatc ggagtccagc tcttcaaggg
     7201 gaagctgtac acctgttcag acagttccaa acagactgag gctgaatgca agggtaacta
     7261 catcacctac aaagatggag aggttgacca tcccatcatc cagccgcgca gctgggagaa
     7321 cagcaagttt gactttgaca acgtcctggc agccatgatg gccctcttca ctgtctccac
     7381 cttcgagggc tggccagagc tgctgtaccg ctccatcgac tcccacacgg aagacaaggg
     7441 ccctatctac aactaccgag tggagatctc catcttcttc atcatctaca tcatcatcat
     7501 cgccttcttc atgatgaaca tcttcgtggg tttcgtcatt gtcaccttcc aggagcaggg
     7561 ggagcaggag tacaagaact gtgagctgga caagaaccag cggcagtgcg tggaatatgc
     7621 cctcaaggcc cggcccctgc ggaggtacat ccccaagaac cagcaccagt acaaagtgtg
     7681 gtacgtggtc aactccacct actttgagta cctgatgttc gtcctcatcc tgctcaacac
     7741 catctgcttg gccatgcagc actacggcca gagctgcctg ttcaaaatcg ccatgaacat
     7801 cctcaacatg ctcttcaccg gcctcttcac cgtggaaatg atcctgaagc tcattgcctt
     7861 caaacccaag ggttacttta gtgatccctg gaatgttttt gacttcctca tcgtaattgg
     7921 cagcataatt gacgtcattc tcagtgagac taatccagct gaacataccc aatgctctcc
     7981 ctctatgaac gcagaggaga actcccgcat ctccatcacc ttcttccgcc tgttccgggt
     8041 catgcgcctg gtcaagctgc tgagccgcgg ggagggcatc cggacgctgc tgtggacctt
     8101 catcaagtcc ttccaggccc tgccctatgt ggctcttctg atcgtaatgc tgttcttcat
     8161 ctatgctgtg atcgggatgc aggtgtttgg gaaaatcgcc ctgaacgaca ccacggagat
     8221 caaccggaac aacaacttcc agaccttccc ccaagctgtg ctgctcctct tcaggtgtgc
     8281 cacgggggag gcttggcagg atatcatgct ggcctgcatg ccaggcaaga agtgtgcccc
     8341 agagtctgag ccccacaaca gcacagaagg ggagaccccc tgcggcagca gcttcgccgt
     8401 cttctacttc atcagcttct acatgctttg tgccttcctg atcatcaatc tctttgtagc
     8461 tgtcatcatg gacaactttg actacctgac aagggactgg tcaatccttg gtccccacca
     8521 tctggatgaa tttaaaagaa tctgggcaga gtatgaccct gaagccaagg gtcgtatcaa
     8581 acacctggat gtggtgaccc tcctccggcg gattcagccc ccactgggtt ttgggaagct
     8641 gtgccctcac cgtgtggctt gcaaacgcct ggtctccatg aacatgcctc tgaacagtga
     8701 cgggacggtc atgttcaacg ccaccctgtt tgccctggtc aggacagctc tgaggatcaa
     8761 aacagaagga aacctggaac aagccaatga ggagctgcgg gccatcatca agaagatctg
     8821 gaagcggacc agcatgaagc tgctggacca agtggtgccc cctgcaggcg atgatgaggt
     8881 cacagtcggc aagttctacg ctaccttcct gatccaagag tacttccgga aattcaagaa
     8941 gcgcaaagag caagggcttg tgggcaagcc ctcccagagg aatgcccttt ccctgcgggc
     9001 tggcctgcgc actctgcacg acatcgggcc tgagatccga cgggccatct ccggagacct
     9061 gacagctgag gaagagctgg acaaggccat gaaggaggct gtgtctgctg cctctgaaga
     9121 tgacatcttc aggagggccg gtggcctgtt tggcaaccat gtcagctact accaaagtga
     9181 cagccggagc gccttccccc agaccttcac tacgcagcgc ccactgcaca tcagcaaggc
     9241 tggcaacaac caaggcgaca ccgagtcacc ctcccacgag aagctggtgg actccacttt
     9301 cacccccagc agctactcgt ccaccggctc caacgccaac atcaacaatg ccaacaacac
     9361 tgccctgggc cgcctccccc gccccgccgg ctaccccagc acagtcagca ctgtggaggg
     9421 ccacgggtcc cccttgtctc ctgccgtccg ggcacaggag gcagcatgga agctcagctc
     9481 caagagatgc cactcccagg agagccagat agccatggcg tgtcaggagg gcgcatccca
     9541 ggacgacaac tacgacgtga ggatcggtga agatgcagag tgctgcagtg agcccagcct
     9601 gctctccaca gagatgctct cctaccagga tgacgaaaac cgacaactgg cgcccccgga
     9661 ggaggagaag cgggacatca ggctgtctcc aaagaagggt ttcctgcgct ccgcatcact
     9721 gggtcgaagg gcttccttcc acctggagtg tctgaagcgg cagaagaatc aagggggaga
     9781 catctctcag aagacagtcc tgcccctgca tctggtccac caccaggcat tggcagtggc
     9841 gggcctgagt cccctcctgc agagaagcca ttcccccacc tcgctcccta ggccctgtgc
     9901 cacgccccct gccacaccgg gcagccgagg ctggccccca cagcccatcc ccaccctgcg
     9961 gctggagggg gccgactcca gtgagaaact caacagcagc ttcccgtcca tccactgcgg
    10021 ctcatggtct ggggagaaca gcccctgcag aggggacagc agcgccgccc ggagagcccg
    10081 gcccgtctcc ctcactgtgc ccagccaggc tggggcccag gggagacagt tccatggcag
    10141 cgccagcagc ctggtggaag cggtcttgat ttccgaagga ctggggcagt ttgctcaaga
    10201 tcccaagttc atcgaggtca cgacccagga gctggctgac gcctgcgatc tgaccataga
    10261 ggagatggag aacgcggccg acgacattct cagcgggggc gcccggcaga gccccaatgg
    10321 caccctgtta ccctttgtga accgcaggga cccgggccgg gacagagcgg ggcagaacga
    10381 gcaggacgcg agcggcgcat gcgccccagg gtgcgggcag agcgaggagg ccctcgcgga
    10441 ccgcagggcc ggcgtcagca gcctgtaggt taacaagctt cgaattctgc agtcgacaat
    10501 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
    10561 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
    10621 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
    10681 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
    10741 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
    10801 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
    10861 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
    10921 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
    10981 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
    11041 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgctgag gccggccgcg
    11101 gccgcatcga tgccgtatac ggtaccccta agaacaatga gttacaaatt ggcaatagac
    11161 atgtctcatt ttataaaaga aaagggggga ctggaaggga tttattatag tgcaagaaga
    11221 caagatctgc attttgctat gtagtcagtc gctctgcgga gaggctggca gattgagccc
    11281 tgggaggttc tctccagcac tagcaggtag agcctgggtg ttccctgcta gactctcacc
    11341 agcacttggc cggtgctggg cagagtgact ccacgcttgc ttgcttaaag ccctcttcaa
    11401 taaagcttca gctgctcgag ctagcagatc tttttccctc tgccaaaaat tatggggaca
    11461 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
    11521 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
    11581 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg
    11641 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
    11701 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
    11761 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
    11821 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg
    11881 acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
    11941 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    12001 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    12061 aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa
    12121 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
    12181 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
    12241 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc
    12301 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    12361 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg
    12421 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
    12481 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    12541 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    12601 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    12661 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    12721 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    12781 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    12841 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    12901 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    12961 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    13021 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    13081 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    13141 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    13201 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    13261 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    13321 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    13381 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    13441 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    13501 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    13561 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    13621 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    13681 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    13741 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    13801 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    13861 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    13921 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    13981 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    14041 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    14101 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    14161 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    14221 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    14281 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    14341 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    14401 ccacctgg
//
