LOCUS       sequence-47125-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3040-01
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..760
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
ORIGIN
        1 ggatccccgg gttattaacg gtgacggtgc tggtttaatt aacatgtcta aaggtgaaga
       61 attattcact ggtgttgtcc caattttggt tgaattagat ggtgatgtta atggtcacaa
      121 attttctgtc tccggtgaag gtgaaggtga tgctacttac ggtaaattga ccttaaaatt
      181 tatttgtact actggtaaat tgccagttcc atggccaacc ttagtcacta ctttaactta
      241 tggtgttcaa tgtttttcta gatacccaga tcatatgaaa caacatgact ttttcaagtc
      301 tgccatgcca gaaggttatg ttcaagaaag aactattttt ttcaaagatg acggtaacta
      361 caagaccaga gctgaagtca agtttgaagg tgatacctta gttaatagaa tcgaattaaa
      421 aggtattgat tttaaagaag atggtaacat tttaggtcac aaattggaat acaactataa
      481 ctctcacaat gtttacatca tggctgacaa acaaaagaat ggtatcaaag ttaacttcaa
      541 aattagacac aacattgaag atggttctgt tcaattagct gaccattatc aacaaaatac
      601 tccaattggt gatggtccag tcttgttacc agacaaccat tacttatcca ctcaatctgc
      661 cttatccaaa gatccaaacg aaaagagaga ccacatggtc ttgttagaat ttgttactgc
      721 tgctggtatt acccatggta ttgatgaatt gtacaaataa ggcgcgccac ttctaaataa
      781 gcgaatttct tatgatttat gatttttat
//
