LOCUS       sequence-471250-       14714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of voltage-gated calcium channel, CaV2.1, 
            N-terminally tagged with mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14714)
  AUTHORS   Benedetti L, Fan R, Weigel AV, Moore AS, Houlihan PR, Kittisopikul 
            M, Park G, Petruncio A, Hubbard PM, Pang S, Xu CS, Hess HF, Saalfeld
            S, Rangaraju V, Clapham DE, De Camilli P, Ryan TA, 
            Lippincott-Schwartz J
  TITLE     Periodic ER-plasma membrane junctions support long-range Ca(2+) 
            signal integration in dendrites.
  JOURNAL   Cell. 2025 Jan 23;188(2):484-500.e22. doi: 
            10.1016/j.cell.2024.11.029. Epub 2024 Dec 20.
  PUBMED    39708809
REFERENCE   2  (bases 1 to 14714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.11.029"; journalName: "Cell"; date: 
            "2025-01-23- 23"; volume: "188"; issue: "2"; pages: "484-500.e22"
COMMENT     Sequence Label: pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.1 WPRE
FEATURES             Location/Qualifiers
     source          1..14714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2694..3141
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3164..3173
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3170..3172,3173..3175,3176..3886)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3476..3495)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3823..3844
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             10607..10633
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     misc_feature    10804..11392
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10857..10877)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11275..11286)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11735..11754)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    11800..11855
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(11854..11873)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(12216..12232)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12216..12232)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12229..12251)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12240..12256
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12264..12270,12271..12288,12289..12294))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12309..12330
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12389..12584
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12435..12570
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    12590..12724
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12627..12646)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12681..12700
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12792..12809)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12963..13551)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13043..13062)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13722..14513,14514..14582))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14345..14364
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14583..14687)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgatcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacccttctt aacatggctg acaagaagga aactagatga gacagcaggg actttccaca
      421 aggggtgtta cggggaggta ctggggagga gccggtcggg aacacccact ttcttgatgt
      481 ataaatatca ctgcattttg ctatgtagtc agtcgctctg cggagaggct ggcagattga
      541 gccctgggag gttctctcca gcactagcag gtagagcctg ggtgttccct gctagactct
      601 caccagcact tggccggtgc tgggcagagt gactccacgc ttgcttgctt aaagccctct
      661 tcaataaagc tgccatttta gaagtaagct agtgtgtgtt cccatctctc ctagtcgccg
      721 cctggtcaac tcggtactcg gtaataagaa gaccctggtc tgttaggacc ctttctgctt
      781 tgggaaaccg aagcaggaaa atccctagca gattggcgcc cgaacaggga cttgaaggag
      841 agtgagagac tcctgagtac ggctgagtga aggcagtaag ggcggcagga accaaccacg
      901 acggagtgct cctataaagg cgcgggtcgg taccagacgg cgtgaggagc gggagaggaa
      961 gaggcctccg gttgcaggta agtgcaacac aaaaaagaaa tagctgtctt ttatccagga
     1021 agggataata agatagagtg ggagatgggc gcgagaaact ccgtcttgtc agggaagaaa
     1081 gcagatgaat tagaaaaaat taggctacga cccaacggaa agaaaaagta catgttgaag
     1141 catgtagtat gggcagcaaa tgaattagat agatttggat tagcagaaag cctgttggag
     1201 aacaaagaag gatgtcaaaa aatactttcg gtcttagctc cattagtgcc aacaggctca
     1261 gaaaatttaa aaagccttta taatactgtc tgcgtcatct ggtgcattca cgcagaagag
     1321 aaagtgaaac acactgagga agcaaaacag atagtacaga gacacagagg aagagcacca
     1381 tacaatccac agagtcaggg agtagtggaa gcaatgaatc accacctgaa aaatcaaata
     1441 gatagaatca gggaacaagc aaattcagta gaaaccatag tattaatggc agttcattgc
     1501 atgaatttta aaagaagggg aggaataggg gatatgactc cagcagaaag attaattaac
     1561 atgatcacta cagaacaaga aatacaattt caacaatcaa aaaactcaaa atttaaaaat
     1621 tttcgggtct attacagaga aggcagagat caactgtgga agggacccgg tgagctattg
     1681 tggaaagggg aaggagcagt catcttaaag gtagggacag acattaaggt agtacccaga
     1741 agaaaggcta aaattatcaa agattatgga ggaggaaaag agatggatag cagttcccac
     1801 atggaggata ccggagaggc tagagaggtg gcatagcctc atacaattcg attggaattg
     1861 ggagattata aattagtaga gatcactcca attggcttgg cccccacaaa tgtgaggagg
     1921 tacactactg gtggcacctc aagaaataaa agaggggtct ttgtgctagg gttcttgggt
     1981 tttctcgcaa cggcaggttc tgcaatgggc gcggcgtcgt tgacgctgac cgctcagtcc
     2041 cggactttat tggctgggat agtgcagcaa cagcaactgc tgttggacgt ggtcaagaga
     2101 caacaagaat tgttgcgact gaccgtctgg ggaacaaaga acctccagac tagggtcact
     2161 gccatcgaga aatacttaaa ggaccaggcg cagctgaatg cttggggatg tgcgtttaga
     2221 caagtctgcc acactactgt accatggcca aatgcaagtc taacaccaaa gtggaacaat
     2281 gagacttggc aagagtggga gcgaaaggtt gacttcttgg aggaaaatat aacagccctc
     2341 ctagaggagg cacaaattca acaagagaag aacatgtatg aattacaaaa gttaaatagc
     2401 tgggatgtgt ttggcaattg gtttgacctt gcttcttgga taaagtatat acaatatgga
     2461 gtttatatag ttgtaggagt aatactgtta agaatagtaa tctatatagt acaaatgcta
     2521 gctaagttaa ggcaggggta taggccagtg ttctcttccc caccctctta tttccagtag
     2581 acccatatcc aacaggacca ggcactgcca accagagaag gcaaagaagg agacggtgga
     2641 gaaggcggtg gttcgaacgc gccgtttcta gactgcagag ggccctgcgt atgagtgcaa
     2701 gtgggtttta ggaccaggat gaggcggggt gggggtgcct acctgacgac cgaccccgac
     2761 ccactggaca agcacccaac ccccattccc caaattgcgc atcccctatc agagaggggg
     2821 aggggaaaca ggatgcggcg aggcgcgtgc gcactgccag cttcagcacc gcggacagtg
     2881 ccttcgcccc cgcctggcgg cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg
     2941 tcactcgccg gtcccccgca aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc
     3001 gccggcccag ccggaccgca ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat
     3061 ctgcgctgcg gcgccggcga ctcagcgctg cctcagtctg cggtgggcag cggaggagtc
     3121 gtgtcgtgcc tgagagcgca gtcgagaaac ccgggaaccg gtcgccacca tggtgagcaa
     3181 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     3241 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     3301 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     3361 cttgacctac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     3421 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     3481 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     3541 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     3601 caactacaac agccacaagg tctatatcac cgccgacaag cagaagaacg gcatcaaggt
     3661 gaacttcaag acccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     3721 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     3781 ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     3841 cgtgaccgcc gccgggatca ctctcggcat ggacgagctc tacaagtccg gactcagatc
     3901 tcgagctcaa gcttcgaatt ctgcagtcga cggtatcgat aagctttgca gaatggcccg
     3961 tttcggagac gagatgcctg cccgctacgg gggaggaggc gccggggcag ccgccggggt
     4021 ggtcgtgggc gccgcaggcg ggcgaagagc cgggggcagc cggcagggcg ggcagcccgg
     4081 ggcgcaaagg atgtacaagc aatccatggc gcagaggaca cggaccatgg ccctgtacaa
     4141 ccccatcccg gtccgacaga actgcctcac ggtcaaccgc tccctcttcc tcttcagcga
     4201 ggacaacgtg gtgagaaaat acgcgaagaa gatcaccgaa tggcctccct ttgagtacat
     4261 gattttagcc accatcatag caaactgcat tgtcctggcg ctggagcagc atctgccaga
     4321 tgacgacaag accccgatgt ctgagcggct ggatgacaca gaaccgtact tcattggcat
     4381 tttttgtttc gaggctggaa ttaaaattat cgccctgggg tttgccttcc acaaagggtc
     4441 ctacctaagg aacggttgga acgtgatgga ctttgtggtg gtgctaactg gcatcttggc
     4501 gacagttggg acagagtttg accttcggac actgcgagca gtgcgggtgc tgcggccgct
     4561 caagctggtg tctggaatcc caagtttaca agtcgtcttg aagtcaatca tgaaggcgat
     4621 gattcctctg ctgcagatcg gactcctcct attttttgca atacttattt ttgcaatcat
     4681 agggttagaa ttttatatgg gaaaatttca taccacctgc tttgaagagg ggacagacga
     4741 cattcagggg gagtctccgg ctccatgcgg gacagaggaa cccgcccgca cctgccccaa
     4801 tgggaccaga tgccagccct actgggaagg acccaacaat gggatcaccc agttcgacaa
     4861 catcctgttc gccgtactga ctgtttttca gtgcatcaca atggaaggat ggacggacct
     4921 cctttacaac agcaacgatg cctcagggaa cacttggaac tggttgtact tcatccccct
     4981 catcatcatc ggctcctttt ttatgctgaa cctcgtgctg ggtgtgctgt caggggagtt
     5041 tgccaaagaa agggagcggg tggagaaccg gcgcgcattc ctgaagctgc ggcggcagca
     5101 gcagattgaa cgcgagctca acgggtacat ggagtggatc tcaaaagcag aagaggtgat
     5161 cctcgcagag gacgagaccg acgtggagca gagacatccc tttgatggag ctctgcggag
     5221 agccactatc aagaagagca agacggacct gctccaccca gaggaggcgg aggatcagct
     5281 ggccgacatc gcctccgtgg ggtctccctt tgcccgagcc agcattaaaa gtgccaagct
     5341 ggagaactcg agttttttcc acaaaaaaga gaggagaatg cgtttctaca tccgtcgcat
     5401 ggtcaaaact caggccttct actggactgt gctcagtctg gtagccctca acacgctgtg
     5461 cgtggctatc gtccattaca accagcccga gtggctctcc gacttcctgt actatgcaga
     5521 attcattttc ttaggactct ttatgtccga aatgtttata aaaatgtacg ggcttgggac
     5581 gcggccttac ttccactcct ctttcaactg ctttgactgt ggggtcatca tcggcagtat
     5641 ctttgaagtc atctgggctg tcataaaacc cggcacgtcc tttgggatca gcgtgttacg
     5701 agccctcagg ttactgcgta ttttcaaagt cacaaagtac tgggcgtctc tccgaaacct
     5761 ggtcgtgtca cttctcaact ccatgaaatc catcatcagt ctgctgttcc tcctcttcct
     5821 gtttatcgtt gtctttgcac ttctggggat gcagctcttc ggtggccagt ttaatttcga
     5881 cgaaggaact cctcccacca actttgacac ctttccggca gcaataatga cggtgtttca
     5941 gatcctgacg ggagaggact ggaacgaggt catgtacgac ggcatcaagt cgcagggcgg
     6001 cgtgcagggc ggcatggtgt tctccatcta cttcatcgtc ctcacactct ttgggaacta
     6061 caccctcctg aatgtgttct tggccatcgc cgtggacaac ctggccaatg cccaggagct
     6121 caccaaggat gagcaagagg aagaggaggc cgtcaatcaa aagcttgccc tgcagaaggc
     6181 caaggaggtg gcggaagtga gtcccctgtc tgcagccaac atgtccatag ccatgaagga
     6241 acagcaaaag aaccagaagc cggccaagtc ggtgtgggag cagcggacca gcgagatgcg
     6301 gaaacagaac ctgctggcca gccgcgaggc cctgtacagc gagatggacc cggaggagcg
     6361 ctggaaggcg tcgtacgcgc gccacctgcg gccggacatg aaaacgcacc tggaccggcc
     6421 gctggtcgtg gacccgcagg agaaccgcaa caacaacacc aacaagagcc gcgtggccga
     6481 gcccacggtg gaccagcgcc tcgggcagca gcgggccgag gacttcctcc ggaagcaggc
     6541 ccgccaccac gatcgcgcgc gggaccccag cgcccacgcc gccgccggcc tggacgcgcg
     6601 caggccctgg gcgggcagcc aggaggccga gctgagccgg gaggggccgt acggccgcga
     6661 gtcggaccac caggcccggg agggcggcct ggagcctccg ggcttctggg agggcgaggc
     6721 ggagcggggc aaggctgggg acccgcaccg caggcacgcg caccggcaag gcgttggcgg
     6781 cagcgggggt agccgcagcg gctcgccccg caccggcacc gccgacgggg agccccgcag
     6841 gcaccgggtc catcgcaggc ccggagagga cggaccggac gacaaggccg aaaggagggg
     6901 ccggcatcgc gagggcagcc ggccagcccg gagcggcgag ggagaggccg aggggccgga
     6961 cggcggtggc ggtggtggtg gcgaacgcag gaggcggcat cggcatggcc ccccgcccgc
     7021 ctacgacccc gacgcgcgga gggacgacag ggaacgcagg caccggagga ggaaagacac
     7081 ccagggctcc ggcgtccccg tgtccggccc caacctctcc accacccggc cgatccagca
     7141 ggatctgagc cgccaggagc ccccgctggc cgaggacatg gacaacctga agaacagccg
     7201 gctagccacc gccgagccgg tcagccccca cgagaacctc agccacgccg gcctgcccca
     7261 gagcccggcc aagatgggga gcagcaccga ccccgccggc cccacgccgg ccacggccgc
     7321 caacccccag aacagcaccg ccagccgccg cacacccaac aacccgggga acccgtccaa
     7381 ccccggcccc cccaagaccc ccgagaacag ccttatcgtc accaacccca gcaccgccca
     7441 aaccaactca gccaagactg ccaggaaacc tgaccacacc acggtggaaa tcccaccagc
     7501 ctgcccaccg cccctcaacc acaccgtggt ccaagtgaac aaaaatgcca acccggaccc
     7561 actaccaaag aaagaagatg agaagaagga ggaggtggac gagggccctg gggaagacgg
     7621 ccccaagccc atgcctccct acagttccat gtttatcctg tccaccacca acccccttcg
     7681 ccgcctgtgc cattacatcc tgaacctgcg ctacttcgag atgtgcatcc tcatggtcat
     7741 cgccatgagc agcatcgccc tggccgccga ggaccccgtg cagcccaatg cgccgcgcaa
     7801 caacgtgctg cgatatttcg actacgtttt cactggcgtc tttacctttg agatggtgat
     7861 caagatgatc gacttagggc ttgtcctgca tcaaggtgcc tacttccggg acctctggaa
     7921 cattctcgac ttcatagtgg tcagtggggc cctggtggcc tttgctttca ccggcaatag
     7981 caaagggaag gatataaaca cgatcaaatc cctccgagtg ctccgggtgc tacgacctct
     8041 caaaaccatc aagcggttgc caaagctcaa ggccgtgttc gactgcgtgg tgaactcact
     8101 caaaaacgtc ttcaacatcc tcattgtgta catgctgttc atgttcatct tcgccgtggt
     8161 ggctgtccag ctgttcaagg gcaagttttt tcactgcacc gatgagtcca aggaatttga
     8221 gaaagattgt cgcggcaagt acctcctgta cgagaagaac gaggtgaaag cccgcgatcg
     8281 cgagtggaag aagtacgagt ttcactatga caacgtgctg tgggccctgc tcaccctctt
     8341 caccgtgtcc acgggagaag gctggccaca ggtcctcaag cactcggtgg acgccacctt
     8401 tgaaaaccag ggccccagcc ccggctaccg catggagatg tccatcttct acgtggtcta
     8461 cttcgtcgtc ttccctttct tctttgtcaa catcttcgtg gccttgatca tcatcacctt
     8521 ccaggagcag ggcgacaaga tgatggagga gtacagcttg gagaaaaacg agagggcctg
     8581 catcgacttc gccatcagtg ccaagccgct gaccaggcac atgccccaga acaagcagag
     8641 cttccagtac cgcatgtggc agttcgtggt gtccccgccc ttcgagtaca ccatcatggc
     8701 catgatcgcc ctcaacacca tcgtgctgat gatgaagttc tacggagcct cagtggccta
     8761 cgacaatgcc ctgaaggtat tcaacatcgt cttcacctcg ctcttctccc tggagtgcct
     8821 gctaaaggtc ctggcttttg ggattctgaa ttatttccgc gatgcctgga atatctttga
     8881 ctttgtgacc gtcctcggta gcatcaccga catccttgtg acggagtttg ggaacaactt
     8941 catcaacctg agcttcctgc gcctcttccg cgccgcgcgg ctcatcaagc tcctgcggca
     9001 gggctacacc atccgcatcc tcctctggac gttcgtgcag tccttcaagg cgctgcccta
     9061 cgtctgcctg ctgatcgcca tgctcttctt catctacgcc atcatcggga tgcaggtgtt
     9121 cggcaacatc ggcatcgaca tggaggacga ggacagcgac gaggacgagt tccagatcac
     9181 cgagcacaat aacttccgga ccttcttcca ggccctcatg ctgctcttcc ggagcgccac
     9241 cggagaagcc tggcacaaca tcatgctgtc ctgcctcagc gggaagccgt gcgacaagaa
     9301 ctcgggcatc ctgacgcctg agtgcggcaa cgaatttgcc tatttttact tcgtgtcctt
     9361 catcttcctc tgctccttcc tgatgttgaa cctctttgtg gccgtcatca tggacaactt
     9421 cgagtacctc acgcgcgact cctccatcct gggcccccac cacctggatg aatacgtgcg
     9481 ggtctgggcc gagtacgacc ctgctgcttg gggacgcatg ctttaccggg acatgtatgc
     9541 gatgctgaga cacatgcccc cacccctggg tctggggaag aactgcccgg ccagagtggc
     9601 ctacaagagg ctcctgcgga tggatctgcc cgtggccgac gacaacactg tgcacttcaa
     9661 ctccacactc atggctttga tccgtacggc gctggacatc aagatcgcca agggcggagc
     9721 agataaacag cagatggacg ctgagctgcg caaagagatg atggccattt ggcccaacct
     9781 gtcccagaag acactggacc tgctggtcac accccacaag tccacggacc tgacagtggg
     9841 caagatctat gccgccatga tgatcatgga gtactaccgg cagagcaaag ccaaaaagct
     9901 gcaggccatg cgcgaggagc agaaccggac accgctcatg ttccagcgca tggagccccc
     9961 gccggatgag gggggcgccg gccagaacgc cctgccctcc acgcagctgg acccggcagg
    10021 aggcctgatg gctcacgaag acggcctcaa agacagccca tcctgggtga cccagcgcgc
    10081 tcaggagatg ttccagaaga cgggcacgtg gagcccggag cgcgcgcccc ccgccgacat
    10141 ggccgacagc cagcccaagc ctcagtccgt ggagatgcga gaaatgagcc aggacggcta
    10201 ctcggacagc gagcactgcc tccccatgga aggccaggcc cgcgccgcct ctatgccccg
    10261 cctccccgcc gagaaccaga ggagaagggg ccggccacgt gggagtgatc tcagtaccat
    10321 ctgtgacacc agccccatga agcgctcggc ctccgtgctg ggccccaagg cgtcccggcg
    10381 cctggacgac tactccctgg agcgcgtccc acccgaggag aaccagcgac accacccgcg
    10441 gcgtcgcgag cgcgcgcatc gcacctccga gcgctccctg ggccgctaca ccgacgtgga
    10501 cacaggcttg ggcacggacc tcagcatgac cacccagtcc ggggacctgc cgtccagaga
    10561 gcgagagcag gagcgcggcc ggcccaagga ccggaagcac cggccgcatc accaccacca
    10621 ccaccaccac caccccggcc cggcggccgc cgacaaggaa cgctacggcc cgcaggaccg
    10681 gcccgaccat ggccacggcc gcgcccgcgc ccgggaccag cgctggtccc gctcgcccag
    10741 cgagggccgg gagcacacca cgcaccggca gtaggttaac aagcttcgaa ttctgcagtc
    10801 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
    10861 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
    10921 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
    10981 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
    11041 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
    11101 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
    11161 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
    11221 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
    11281 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
    11341 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gctgaggccg
    11401 gccgcggccg catcgatgcc gtatacggta cccctaagaa caatgagtta caaattggca
    11461 atagacatgt ctcattttat aaaagaaaag gggggactgg aagggattta ttatagtgca
    11521 agaagacaag atctgcattt tgctatgtag tcagtcgctc tgcggagagg ctggcagatt
    11581 gagccctggg aggttctctc cagcactagc aggtagagcc tgggtgttcc ctgctagact
    11641 ctcaccagca cttggccggt gctgggcaga gtgactccac gcttgcttgc ttaaagccct
    11701 cttcaataaa gcttcagctg ctcgagctag cagatctttt tccctctgcc aaaaattatg
    11761 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca
    11821 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
    11881 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc
    11941 tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg
    12001 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt
    12061 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca
    12121 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat
    12181 ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
    12241 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
    12301 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
    12361 tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc
    12421 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    12481 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
    12541 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat
    12601 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    12661 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    12721 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    12781 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    12841 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    12901 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    12961 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    13021 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    13081 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    13141 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    13201 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    13261 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    13321 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    13381 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    13441 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    13501 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    13561 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    13621 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    13681 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    13741 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    13801 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    13861 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    13921 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    13981 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    14041 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    14101 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    14161 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    14221 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    14281 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    14341 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    14401 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    14461 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    14521 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    14581 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    14641 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    14701 aaagtgccac ctgg
//
