LOCUS       sequence-471313-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression and nuclear localization of mTagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Goldman, C., Kareva, T., Sarrafha, L., Schuldt, B.R., Sahasrabudhe, 
            A., Ahfeldt, T.,, Blanchard, J.W
  TITLE     Genetically encoded and modular subcellular organelle probes reveal 
            dysfunction in lysosomes and mitochondria driven by PRKN knockout
  JOURNAL   iScience 28 (2025)
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience 28
            (2025)"
COMMENT     Sequence Label: pFUW H2B-mTagBFP2-P2A-PuroR
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..378
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      391..400
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgccagagc cagcgaagtc tgctcccgcc ccgaaaaagg gctccaagaa ggcggtgact
       61 aaggcgcaga agaaaggcgg caagaagcgc aagcgcagcc gcaaggagag ctattccatc
      121 tatgtgtaca aggttctgaa gcaggtccac cctgacaccg gcatttcgtc caaggccatg
      181 ggcatcatga attcgtttgt gaacgacatt ttcgagcgca tcgcaggtga ggcttcccgc
      241 ctggcgcatt acaacaagcg ctcgaccatc acctccaggg agatccagac ggccgtgcgc
      301 ctgctgctgc ctggggagtt ggccaagcac gccgtgtccg agggtactaa ggccatcacc
      361 aagtacacca gcgctaagga tccaccggta gccaccatgg tgtctaaggg cgaagagctg
      421 attaaggaga acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc
      481 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
      541 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac
      601 ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc
      661 cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct
      721 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg
      781 aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc
      841 gagacgctgt accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc
      901 gtgggcggga gccatctgat cgcaaacgcc aagaccacat atagatccaa gaaacccgct
      961 aagaacctca agatgcctgg cgtctactat gtggactaca gactggaaag aatc
//
