LOCUS       sequence-471314-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression and nuclear localization of mTagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Goldman, C., Kareva, T., Sarrafha, L., Schuldt, B.R., Sahasrabudhe, 
            A., Ahfeldt, T.,, Blanchard, J.W
  TITLE     Genetically encoded and modular subcellular organelle probes reveal 
            dysfunction in lysosomes and mitochondria driven by PRKN knockout
  JOURNAL   iScience 28 (2025)
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience 28
            (2025)"
COMMENT     Sequence Label: pFUW H2B-mTagBFP2-P2A-PuroR
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             376..432
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             433..1032
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(433..452)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     929..949
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa
       61 acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc
      121 agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa cgccaagacc
      181 acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac
      241 tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg
      301 gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa tgatctgcag
      361 tctagaggat ctggtgccac gaatttctca ctactcaagc aggccggtga tgtcgaggaa
      421 aaccctggtc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
      481 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
      541 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
      601 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
      661 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
      721 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
      781 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
      841 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
      901 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
      961 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     1021 cccggtgcct ga
//
