LOCUS       sequence-47132-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Parker JB, Palchaudhuri S, Yin H, Wei J, Chakravarti D
  TITLE     A Transcriptional Regulatory Role of the THAP11-HCF-1 Complex in 
            Colon Cancer Cell Function.
  JOURNAL   Mol Cell Biol. 2012 May;32(9):1654-70. Epub 2012 Feb 27.
  PUBMED    22371484
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2012-05"; volume: "32(9)"; pages: "1654-70. Epub 2012
            Feb 27"
COMMENT     Sequence Label: pBABE-EGFP-THAP11-rescue-3xFLAG
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(26..355)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      40..175
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     340..360
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(379..444)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 cgcccttgct accatggtga agctttttgc aaaagcctag gcctccaaaa aagcctcctc
       61 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
      121 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
      181 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
      241 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
      301 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
      361 acagggtcga catcactact tgtcatcgtc atccttgtag tcgatgtcat gatctttata
      421 atcaccgtca tggtctttgt agtcagcccg ggatcctcta gacattccgt gcttcttgcg
      481 gatgacagcc atggcaagca gccgatcctt ctcgcggagc tcttcgcgca gcttggcctc
      541 agtgagacgc aggtggcgaa tgctgccttt catctctttc attttgacct ccatcagagc
      601 caggatgtcc cgctgctcat tcagcttgcg caggagctcc tcctccgtgg tgcctgacga
      661 caaggagtac gaatggtcgg agccagtatc agggaagccc tcttccccta ccaccaccaa
      721 ctgggggccc atagggcact cggcagcctc cagccctgcg gtagcagcct gtaactccga
      781 cgcggcggcc gcagcggctg cgccctctgc ggctgcaaac tccacttgca ctgtgagatc
      841 gatgggcttc acgtcttctc cagtgggant gatgggcgcc ggctgtacgg atcccgga
//
