LOCUS       sequence-471320-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression and lysosomal localization of mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Goldman, C., Kareva, T., Sarrafha, L., Schuldt, B.R., Sahasrabudhe, 
            A., Ahfeldt, T.,, Blanchard, J.W
  TITLE     Genetically encoded and modular subcellular organelle probes reveal 
            dysfunction in lysosomes and mitochondria driven by PRKN knockout
  JOURNAL   iScience 28 (2025)
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience 28
            (2025)"
COMMENT     Sequence Label: pFUW LAMP1Sig-mCherry
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..816
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(251..269)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     519..538
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggcggccc ccggcagcgc ccggcgaccc ctgctgctgc tactgctgtt gctgctgctc
       61 ggcctcatgc attgtgcgtc agcaggggat ccaccggtcg ccaccatggt gagcaagggc
      121 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      181 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      241 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      301 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      361 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      421 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      481 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      541 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
      601 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
      661 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
      721 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
      781 cactccaccg gcggcatgga cgagctgtac aagtaa
//
