LOCUS       sequence-471328-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cytosolic expression of mTagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Goldman, C., Kareva, T., Sarrafha, L., Schuldt, B.R., Sahasrabudhe, 
            A., Ahfeldt, T.,, Blanchard, J.W
  TITLE     Genetically encoded and modular subcellular organelle probes reveal 
            dysfunction in lysosomes and mitochondria driven by PRKN knockout
  JOURNAL   iScience 28 (2025)
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience 28
            (2025)"
COMMENT     Sequence Label: pFUW mTagBFP2-P2A-PuroR
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7..717
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             742..798
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(799..818)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gccaccatgg tgtctaaggg cgaagagctg attaaggaga acatgcacat gaagctgtac
       61 atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc
      121 tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc
      181 ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag
      241 ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc
      301 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc
      361 ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag
      421 aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga cggcggcctg
      481 gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacgcc
      541 aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat
      601 gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac
      661 gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa gcttaatgat
      721 ctgcagtcta gaggatctgg tgccacgaat ttctcactac tcaagcaggc cggtgatgtc
      781 gaggaaaacc ctggtcctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
      841 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
      901 cacnccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
      961 acgcgcgtcg ggctcgacat cggcaaggtg
//
