LOCUS       sequence-471339-        1191 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cytosolic expression of mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Goldman, C., Kareva, T., Sarrafha, L., Schuldt, B.R., Sahasrabudhe, 
            A., Ahfeldt, T.,, Blanchard, J.W
  TITLE     Genetically encoded and modular subcellular organelle probes reveal 
            dysfunction in lysosomes and mitochondria driven by PRKN knockout
  JOURNAL   iScience 28 (2025)
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience 28
            (2025)"
COMMENT     Sequence Label: pFUW mCherry-P2A-NeoR
FEATURES             Location/Qualifiers
     source          1..1191
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             265..321
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             316..1116
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(376..395)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     986..1005
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
       61 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
      121 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
      181 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
      241 gatctgcagt ctagaggatc tggtgccacg aatttctcac tactcaagca ggccggtgat
      301 gtcgaggaaa accctggtcc tatgattgaa caagatggat tgcacgcagg ttctccggcc
      361 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
      421 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
      481 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
      541 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
      601 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
      661 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
      721 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
      781 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
      841 ctcaaggcgc gcatgcccga cggcgatgat ctcgtcgtga cccatggcga tgcctgcttg
      901 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
      961 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     1021 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     1081 atcgccttct atcgccttct tgacgagttc ttctgacgaa ttcgatatca agcttatcga
     1141 taatcaacct ctggattaca aaatttgtga aagattgact ggtatctann c
//
