LOCUS       sequence-471373-       10292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac-integrated plasmid containing hygromycin resistance gene, 
            ZNF35/IKZF1-driven BFP, and GFP-NZF dummy transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10292)
  AUTHORS   Wolfsberg E, Paul J-P, Tycko J, Chen B, Bassik MC, Bintu L, Alizadeh
            AA, Gao XJ
  TITLE     Machine-Guided Dual-Objective Protein Engineering for Deimmunization
            and Therapeutic Functions
  JOURNAL   bioRxiv 2025.02.24.639918
REFERENCE   2  (bases 1 to 10292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.02.24.639918"
COMMENT     Sequence Label: EW375 (PB) (ZNF35/IKZF1tar-6bp spacers)x4-H2B-...
FEATURES             Location/Qualifiers
     source          1..10292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(170..961,962..1030))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     793..812
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1031..1135)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1161..1616)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1298..1319)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1510..1529
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1743..1765
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1757..1774
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1758..1774
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1904..1966
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     5'UTR           2600..2642
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             2691..3068
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             3087..3794
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     polyA_signal    3828..4035
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4068..4088)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4073..4402
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4253..4388
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4315..4334
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4451..5476
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4451..5476
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(5536..5556)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5598..5719
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5635..5654)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5689..5708
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        6214..6593
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6594..6797
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6748..6768
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    6799..6817
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6820..6838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6848..6872
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6964..6973
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6979..6981,6982..7692)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             8206..8235
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    8297..8504
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8776..8810
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9301..9317)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9301..9317)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9314..9336)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9325..9341
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9349..9355,9356..9373,9374..9379))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9394..9415
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9532..9549)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9703..10291)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9783..9802)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
       61 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
      121 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
      181 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
      241 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
      301 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
      361 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
      421 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
      481 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
      541 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
      601 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
      661 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
      721 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
      781 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
      841 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
      901 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      961 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     1021 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     1081 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     1141 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     1201 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     1261 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     1321 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     1381 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     1441 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     1501 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     1561 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
     1621 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     1681 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     1741 ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt
     1801 tgaacccgtg gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca
     1861 gatgaagatg ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca
     1921 tattgtgacg tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct
     1981 gtatatcgag gtttatttat taatttgaat agatattaag ttttattata tttacactta
     2041 catactaata ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac
     2101 aaaaactcaa aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca
     2161 aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg
     2221 ggctccgctc cggtccggcg ctccccccgc atcccagagc cggcagcgtg cggggacagc
     2281 ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg
     2341 agcctgcaga cacctggggg gatacgggga aaaggcctcc acggccacaa ttgacgattg
     2401 agtaaacagg aaatatataa agagctcttc aaacaggaaa tatataaaga ggaggtcaaa
     2461 caggaaatat ataaagagca gaacaaacag gaaatatata aagagagtct agagggtata
     2521 taatggatcc catcgcgtct cagcctcact ttgagctcct ccacacgaat tccccgatac
     2581 cgtcgattca aggagcttgc ttgttctttt tgcagaagct cagaataaac gctcaacttt
     2641 ggcagatcta ccaagcttgg taccgagctc ggatccaact cgaggccacc atgccagagc
     2701 cagcgaagtc tgctcccgcc ccgaaaaagg gctccaagaa ggcggtgact aaggcgcaga
     2761 agaaaggcgg caagaagcgc aagcgcagcc gcaaggagag ctattccatc tatgtgtaca
     2821 aggttctgaa gcaggtccac cctgacaccg gcatttcgtc caaggccatg ggcatcatga
     2881 attcgtttgt gaacgacatt ttcgagcgca tcgcaggtga ggcttcccgc ctggcgcatt
     2941 acaacaagcg ctcgaccatc acctccaggg agatccagac ggccgtgcgc ctgctgctgc
     3001 ctggggagtt ggccaagcac gccgtgtccg agggtactaa ggccatcacc aagtacacca
     3061 gcgctaagga tccccgggta ccggtcgtgt ctaagggcga agagctgatt aaggagaaca
     3121 tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg
     3181 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
     3241 gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct
     3301 tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca
     3361 catgggagag agtcaccaca tacgaggacg ggggcgtgct gaccgctacc caggacacca
     3421 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca
     3481 acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgaa acgctgtacc
     3541 ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc
     3601 atctgatcgc aaacgccaag accacatata gatccaagaa acccgctaag aacctcaaga
     3661 tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaaa
     3721 cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg
     3781 ggcacaagct taattagcta gcgaattcga atttaaatcg gcctcgactg tgccttctag
     3841 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     3901 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     3961 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     4021 caggcatgct ggggacgcgg ccgcaattta acgcgaatta attctgtgga atgtgtgtca
     4081 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4141 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4201 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     4261 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4321 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     4381 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     4441 tcagcacgtg atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
     4501 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
     4561 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
     4621 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
     4681 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     4741 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
     4801 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     4861 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     4921 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     4981 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     5041 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     5101 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     5161 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     5221 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     5281 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     5341 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     5401 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     5461 gagggcaaag gaataggtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     5521 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     5581 gtatcatgcg ttaactaaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5641 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5701 aactcatcaa tgtatcttat catgtctgga attgactcaa atgatgtcaa ttagtctatc
     5761 agaagctcat ctggtctccc ttccggggga caagacatcc ctgtttaata tttaaacagc
     5821 agtgttccca aactgggttc ttatatccct tgctctggtc aaccaggttg cagggtttcc
     5881 tgtcctcaca ggaacgaagt ccctaaagaa acagtggcag ccaggtttag ccccggaatt
     5941 gactggattc cttttttagg gcccattggt atggcttttt ccccgtatcc ccccaggtgt
     6001 ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc
     6061 cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg cggggggagc gccggaccgg
     6121 agcggagccc cgggcggctc gctgctgccc cctagcgggg gagggacgta attacatccc
     6181 tgggggcttt gggggggggc tgtccctgat gttgacattg attattgact agttattaat
     6241 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     6301 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     6361 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     6421 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     6481 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     6541 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     6601 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     6661 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     6721 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     6781 tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc
     6841 gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     6901 acctccatag aagacaccgg gaccgatcca gcctccggac tctagcgttt aaacttaagc
     6961 ttcgccacca tgggatccgt ctcaaagggc gaggaacttt ttacgggcgt tgttccgatc
     7021 ctcgtcgaat tggacgggga cgttaatggg cataaattct cagttagtgg tgagggtgag
     7081 ggggacgcga catatgggaa acttacgctg aagttcatat gtaccaccgg aaaattgcca
     7141 gtgccgtggc ctactctcgt caccaccctg acatatggtg ttcagtgttt tagccggtat
     7201 ccagaccata tgaaacagca cgattttttt aaatccgcta tgcctgaagg ttacgtacag
     7261 gagcgaacga tattttttaa agacgacgga aactacaaaa ctagagccga ggtaaaattc
     7321 gagggtgaca ctctcgtgaa ccggatcgaa ctgaagggca tagattttaa ggaggatggc
     7381 aatatactgg gccacaagct cgaatacaac tataattctc ataatgtcta tatcatggcg
     7441 gacaaacaga aaaacggcat caaggtaaac tttaaaatcc gacacaatat tgaggacggc
     7501 tctgtccagc ttgcggacca ttaccaacag aacacgccga taggggatgg tccagtactt
     7561 ctccctgata atcactattt gtctactcag tcagctctta gcaaggaccc aaacgaaaaa
     7621 cgggatcaca tggtccttct tgagttcgtc actgcggctg ggataacact cgggatggac
     7681 gaactgtata aggctgctgc atgggagcta ggaatgtctg gcggatctga gggccagagc
     7741 gacgagagag ccctgctgga ccagctgcac accctgctga gcaacaccga cgccaccggc
     7801 ctggaggaga tcgacagagc cctgggcatc cccgagctgg tgaaccaggg ccaggccctg
     7861 gagcccaagc aggacggagg ctggggatct atggccgagg aattcgtgac cctgaaggac
     7921 gtgggcatgg acttcaccct gggcgactgg gagcagctgg gcctggagca gggcgacacc
     7981 ttctgggaca ccgccctgga caactgccag gacctgttcc tgctggaccc ccccggcgga
     8041 ggagacggcc ttggtttggg acacgagaag ttccccagcg acctggacct ggacatgttc
     8101 aacggcagcc tggagtgcga catggagagc atcatcagaa gcgagctgat ggacgccgac
     8161 ggcctggact tcaacttcga cagctaggcc gctcgaggag ggcccgaaca aaaactcatc
     8221 tcagaagagg atctgaatag cgccgtcgac catcatcata atcatcattg agtttaaacc
     8281 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     8341 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     8401 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     8461 agcaaggggg aggattggga agacaatagc aggcatgctg gggaatctat aacaagaaaa
     8521 tatatatata ataagttatc acgtaagtag aacatgaaat aacaatataa ttatcgtatg
     8581 agttaaatct taaaagtcac gtaaaagata atcatgcgtc attttgactc acgcggtcgt
     8641 tatagttcaa aatcagtgac acttaccgca ttgacaagca cgcctcacgg gagctccaag
     8701 cggcgactga gatgtcctaa atgcacagcg acggattcgc gctatttaga aagagagagc
     8761 aatatttcaa gaatgcatgc gtcaatttta cgcagactat ctttctaggg ttaatctagc
     8821 tgcatcagga tcatatcgtc gggtcttttt tccggctcag tcatcgccca agctggcgct
     8881 atctgggcat cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa gcggcatgga
     8941 aagagtttgc cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc acgtttacca
     9001 tgatgattcg ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc gttcaggcca
     9061 cctgggatac cagttcgtcg cggcttttcc ggacacagtt ccggatggtc agcccgaagc
     9121 gcatcagcaa cccgaacaat accggcgaca gccggaactg ccgtgccggt gtgcagatta
     9181 atgacagcgg tgcggcgctg ggatattacg tcagcgagga cgggtatcct ggctggatgc
     9241 cgcagaaatg gacatggata ccccgtgagt tacccggcgg gcgcgcttgg cgtaatcatg
     9301 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9361 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9421 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9481 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9541 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9601 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9661 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9721 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9781 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9841 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9901 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9961 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10021 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10081 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10141 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10201 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10261 gcagattacg cgcagaaaaa aaggatctca ag
//
