LOCUS       sequence-471415-        2831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' Homology psbH in the M1 position used for genome integration at 
            the psbH locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_1_001_psbH_5'Hom_Cr
FEATURES             Location/Qualifiers
     source          1..2831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(365..459)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(480..1139)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1054..1073
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1301..1319
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1323..1366)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1369..1390
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2173..2230
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2254..2271)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2425..2831,1..182))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2505..2524)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
       61 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      121 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      181 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      241 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      301 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc
      361 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
      421 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
      481 tacgccccgc cctgccactc gtcgcagtac tgttgtaatt cattaagcat tctgccgaca
      541 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
      601 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
      661 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgatacgaa aaacatattc
      721 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
      781 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagtga tgaaaacgtt
      841 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
      901 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
      961 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     1021 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     1081 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     1141 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1201 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga
     1261 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
     1321 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc
     1381 gcttctagag cgatgagtgg tctcgaacac gtctcgaaca gaattgtcaa tttttaatgt
     1441 acacttagtt gaaagtgccc ctgtcccctt ggccatattt aacagaagtt atttataacg
     1501 cagctgtttt ttggagtcta taaatttata acatcagtta ctatggattt ccctttagtt
     1561 ttatggccta ggacgtcccc ttccccttcg atgctggagg catcctttta cgggacaata
     1621 aataaatttg ttgcctcgcc tatcggctaa caagttcctt cggagtatat aaatatagga
     1681 tgttaatact gctataaact ttagttgccc aatatttata ttaggacgcc agtggcagtg
     1741 gtaccgccac tgcctgcttc gcagtatata aatataggca gttggcaggc aactgccact
     1801 gacgtcctat tttaatactc ccaagtttac ttgcctaggc agttggcagg caacaaattt
     1861 atttattgtc cactaaaatt tatttgcccg aaggggacgt ccactaaaat ttatttaccc
     1921 gaaggggacg tcctaatata aatatgggga tgtcaatgct ccgttaggaa gtaactaacg
     1981 tttttcaaat aaattttatc ccggagggaa gtaggcagta gcccgccact gtcatccttt
     2041 aagtggatct ctcgtcaggc aatttgctta cacctttaaa ttaaaaatta aatttaaaga
     2101 aaagtgagct attaacgcgt ttatcttaac gggagtgaga ccactcatcg cgactagtag
     2161 cggccgctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa
     2221 accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt
     2281 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2341 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2401 taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa
     2461 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2521 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2581 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2641 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2701 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2761 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     2821 tacagagttc t
//
