LOCUS       sequence-471416-        3486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' Homology psbA in the M1 position used for genome integration at 
            the psbA locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_1_004_psbA_5'Hom_Cr
FEATURES             Location/Qualifiers
     source          1..3486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     394..413
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     641..659
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(663..706)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    709..730
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2168..2225
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2249..2266)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2420..3008)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2500..2519)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3191..3285)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3306..3486,1..479))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgatacgaa aaacatattc
       61 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
      121 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagtga tgaaaacgtt
      181 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
      241 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
      301 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
      361 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
      421 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
      481 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
      541 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga
      601 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
      661 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc
      721 gcttctagag cgatgagtgg tctcgaacac gtctcgaaca cctctgttgg agcttctccc
      781 cttcccctta cgggacatcc ccttcccctt acgggatatt tatatactcc gaaggacgtc
      841 cccttcgggc aaataaattt tagtggcagt tgcaaagtat taatatcgta tataaatatc
      901 ctgccaactg cctaggcaag taaacttagg gattttaatg ctccgttagg acgtcccctt
      961 ccccttcggg acgtcccctt acgggaatat aaatattagt ggacgtcccc ttcgggcaaa
     1021 tgaattttag tggcagttgc ctgccaactg ccgatattta tatactgcga taaactttag
     1081 ttgcccgaag gggtttacat acaatttatt tattgtacca ctgccactaa tatttatatt
     1141 cccgtaaggg gacgtcctcc ttccccttcg ggcaagtaaa cttaggagta tgtaaacctg
     1201 ctagcgcagc aaataaattt tattctaagt ttacttgccc gaaggggaag gaggaagcag
     1261 gcagttgcct ccttcccctt ccccttcggg acgtcccctt cgggatttta atgctccgtt
     1321 aggaggcaaa taaattttag tggcagttgc ctgccaactg cctccttcgg agtattaaaa
     1381 taggacgtcc ccttacggga atataaatat tagtggcagt tgcctgccaa ctgcctcctt
     1441 cggagtatta aaataggatg ttaatactgc ggagcaggca gtggcggtac cactgccact
     1501 ggcgtcctcc ttcggagtat gtaaacattc tatatttata tactgcgata aactttagtt
     1561 gcccgaaggg gtttacatac aatttattta ttgtaccact gccactaata tttatattcc
     1621 cgtaagggga cgtcctcctt ccccttcggg caagtaaact taggagtatg taaacctgct
     1681 agcgcagcaa ataaatttta ttctaagttt acttgcccga aggggaagga ggaagcaggc
     1741 agtggtggta ccactgccac taaaatttat ttacccgaag gggacgtcct tccccttccc
     1801 cttcgggacg tcagtggcag ttgcctgcca actgcctaat ataaatattg ggcaagtaaa
     1861 cttaggagta tataaatata ggattttaat actccgaagg aggcagttgg caggcaactg
     1921 ccactgacgt cccgtaaggg gaaggggaca aatttattta ttgtcccgta agggaaagtc
     1981 gtggagtatt taatacagct aaaagactta aagaaacttt agatttagct ataaaactta
     2041 aagcgacagg tacttccgaa acggtggtta ttccaggcca aacttatgaa atgcaagtac
     2101 catcagatat tgctagcatg gatacgggag tgagaccact catcgcgact agtagcggcc
     2161 gctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga
     2221 aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2281 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2341 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2401 aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc
     2461 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2521 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2581 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2641 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2701 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2761 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2821 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2881 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2941 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3001 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3061 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3121 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct
     3181 cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     3241 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
     3301 tcaaattacg ccccgccctg ccactcgtcg cagtactgtt gtaattcatt aagcattctg
     3361 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     3421 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     3481 ttggcc
//
