LOCUS       sequence-471417-        2922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' Homology petB in the M1 position used for genome integration at 
            the petB locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_1_007_petB_5'Hom_cr
FEATURES             Location/Qualifiers
     source          1..2922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      308..402
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      585..1173
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1074..1093
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1327..1344
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1368..1425)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    2299..2320
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2323..2366
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(2370..2388)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(2550..2922,1..287)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2616..2635)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
       61 tatcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
      121 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
      181 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
      241 ttaatgaatt acaacagtac tgcgacgagt ggcagggcgg ggcgtaattt gatatcgagc
      301 tcgcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      361 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcct cgagctgtca
      421 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
      481 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
      541 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
      601 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
      661 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
      721 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
      781 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
      841 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
      901 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
      961 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1021 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1081 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     1141 tgatgctcgt caggggggcg gagcctgtgg aaaaacgcca gcaacgcggc ctttttacgg
     1201 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     1261 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     1321 gagcgcagcg agtcagtgag cgaggaagcc tgcataacgc gaagtaatct tttcggtttt
     1381 aaagaaaaag ggcagggtgg tgacaccttg cccttttttg ccggactgca gcggccgcta
     1441 ctagtcgcga tgagtggtct cactccgcgg tttctgccag atcccccgtt ggcccattga
     1501 taaggggtag ctataaatta accatagtta tattctggtt aaaggatcgg aactaacccc
     1561 aagtctctag tctaaacaaa aaattgtgta tgcatttaac acatttagtg tttttaacta
     1621 gacaaaaaaa attaagtatg atattataaa agtaatattt tttagccttc gtgatggaac
     1681 tggtagacat cctggtttta ggaaccagtg ctgaaaggcg tgccggttca aatccggccg
     1741 aaggcatttt aagtttaacg tagagccaat atttgtttga atttatctat tttttaaacc
     1801 attttggttt aaaattttta tttgcttcaa aggagcctgt aaacggtact ttaattttta
     1861 cagtagcact cgcagagctt atttacgtgc aaataaaagc tctatctact aggatattag
     1921 actagtatta ataaaacaca acattttatt aacaaagtaa tttatgccga ttggagtacc
     1981 tcgtattatt tattgttggg gtgaagaatt acctgcacag tggactgata tttataactt
     2041 tattttccgt cgtcgtatgg tatttttaat gcaatattta gatgatgaat tatgtaacca
     2101 aatttgtggt ttattaatta atatccatat ggaagatcgt tctaaagagt tggaaaaaaa
     2161 agaaatggaa aaaagtggat tatttaaaag tggcaccgca aaaacaaaag gaaaagatac
     2221 agtaaaaaaa gaaaatttat ctggcggcgc tagtgcaaaa cgtcaatctg ttcgagacgt
     2281 gttcgagacc actcatcgct ctagaagcgg ccgcgaattc cagaaatcat ccttagcgaa
     2341 agctaaggat tttttttatc tgaaattctg cctcgtgata cgcctatttt tataggttaa
     2401 tgtcatgata ataatggttt cttagacgtc aggtggcact cgagttgatc gggcacgtaa
     2461 gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat
     2521 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
     2581 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
     2641 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
     2701 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
     2761 cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
     2821 gttacaccgt tttccatgag caaactgaaa cgttttcatc actctggagt gaataccacg
     2881 acgatttccg gcagtttcta cacatatatt cgcaagatgt gg
//
