LOCUS       sequence-471418-        2120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' Connector 1 Short Forward in the M1 position used to dictate 
            position for lvl2 assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_G_0_1_005_5C1SF
FEATURES             Location/Qualifiers
     source          1..2120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             18..677
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(84..103)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      698..792
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      975..1563
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1464..1483
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1717..1734
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1758..1815)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1887..1908
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1911..1954
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1958..1976)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
       61 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
      121 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
      181 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaatttcgta
      241 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
      301 tccatgagca aactgaaacg ttttcatcac tctggagtga ataccacgac gatttccggc
      361 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
      421 ctaaagggtt tattgagaat atgtttttcg tatcagccaa tccctgggtg agtttcacca
      481 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
      541 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
      601 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgacgagt
      661 ggcagggcgg ggcgtaattt gatatcgagc tcgcttggac tcctgttgat agatccagta
      721 atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt
      781 attggtgaga atccaagcct cgagctgtca gaccaagttt actcatatat actttagatt
      841 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
      901 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
      961 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1021 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1081 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     1141 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1201 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     1261 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     1321 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     1381 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     1441 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     1501 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctgtgg
     1561 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     1621 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     1681 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcc
     1741 tgcataacgc gaagtaatct tttcggtttt aaagaaaaag ggcagggtgg tgacaccttg
     1801 cccttttttg ccggactgca gcggccgcta ctagtcgcga tgagtggtct cactcctgtt
     1861 cgagacgtgt tcgagaccac tcatcgctct agaagcggcc gcgaattcca gaaatcatcc
     1921 ttagcgaaag ctaaggattt tttttatctg aaattctgcc tcgtgatacg cctattttta
     1981 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcactcg agttgatcgg
     2041 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
     2101 tgagttatcg agattttcag
//
