LOCUS       sequence-471419-        2120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' Connector 2 Short Forward in the M1 position used to dictate 
            position for lvl2 assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_G_0_1_008_5C2SF
FEATURES             Location/Qualifiers
     source          1..2120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(20..114)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(135..794)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     709..728
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     956..974
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(978..1021)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1024..1045
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1117..1174
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1198..1215)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1369..1957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1449..1468)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
       61 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
      121 agctcgatat caaattacgc cccgccctgc cactcgtcgc agtactgttg taattcatta
      181 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
      241 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
      301 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgat
      361 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
      421 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
      481 agtgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
      541 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg
      601 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
      661 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
      721 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
      781 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
      841 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaac
      901 tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
      961 gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt
     1021 ctggaattcg cggccgcttc tagagcgatg agtggtctcg aacacgtctc gtactggagt
     1081 gagaccactc atcgcgacta gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc
     1141 accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc
     1201 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     1261 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1321 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct
     1381 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1441 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1501 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1561 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1621 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1681 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1741 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1801 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1861 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1921 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1981 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     2041 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     2101 tatatatgag taaacttggt
//
