LOCUS       sequence-471424-        2120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' Connector 1 Short Reverse in the M1 position used to dictate 
            position for lvl2 assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_G_0_1_006_5C1SR
FEATURES             Location/Qualifiers
     source          1..2120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      74..168
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      351..939
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     840..859
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1093..1110
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1134..1191)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1263..1284
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1287..1330
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1334..1352)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(1514..2120,1..53)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1580..1599)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gaatgcttaa tgaattacaa cagtactgcg acgagtggca gggcggggcg taatttgata
       61 tcgagctcgc ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat
      121 ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aagcctcgag
      181 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
      241 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
      301 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      361 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      421 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      481 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      541 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      601 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      661 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      721 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
      781 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
      841 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
      901 tttttgtgat gctcgtcagg ggggcggagc ctgtggaaaa acgccagcaa cgcggccttt
      961 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     1021 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     1081 acgaccgagc gcagcgagtc agtgagcgag gaagcctgca taacgcgaag taatcttttc
     1141 ggttttaaag aaaaagggca gggtggtgac accttgccct tttttgccgg actgcagcgg
     1201 ccgctactag tcgcgatgag tggtctcact cctactcgag acgtgttcga gaccactcat
     1261 cgctctagaa gcggccgcga attccagaaa tcatccttag cgaaagctaa ggattttttt
     1321 tatctgaaat tctgcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     1381 gtttcttaga cgtcaggtgg cactcgagtt gatcgggcac gtaagaggtt ccaactttca
     1441 ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc
     1501 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
     1561 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
     1621 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     1681 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat ttcgtatggc
     1741 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     1801 tgagcaaact gaaacgtttt catcactctg gagtgaatac cacgacgatt tccggcagtt
     1861 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     1921 agggtttatt gagaatatgt ttttcgtatc agccaatccc tgggtgagtt tcaccagttt
     1981 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
     2041 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
     2101 tgatggcttc catgtcggca
//
