LOCUS       sequence-471429-        2120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' Operon Connector 3 in the M1 position used to construct operons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_G_0_1-2_003_OC5'3
FEATURES             Location/Qualifiers
     source          1..2120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      179..767
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     668..687
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     921..938
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(962..1019)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1091..1112
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1115..1158
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1162..1180)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1342..2001
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1408..1427)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2022..2116
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gcctcgagct gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
       61 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
      121 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
      181 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
      241 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
      301 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
      361 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
      421 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
      481 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
      541 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      601 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      661 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      721 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct gtggaaaaac gccagcaacg
      781 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      841 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      901 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcctgcata acgcgaagta
      961 atcttttcgg ttttaaagaa aaagggcagg gtggtgacac cttgcccttt tttgccggac
     1021 tgcagcggcc gctactagtc gcgatgagtg gtctcaagta cattcgagac gtgttcgaga
     1081 ccactcatcg ctctagaagc ggccgcgaat tccagaaatc atccttagcg aaagctaagg
     1141 atttttttta tctgaaattc tgcctcgtga tacgcctatt tttataggtt aatgtcatga
     1201 taataatggt ttcttagacg tcaggtggca ctcgagttga tcgggcacgt aagaggttcc
     1261 aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt
     1321 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
     1381 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
     1441 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
     1501 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattt
     1561 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
     1621 gttttccatg agcaaactga aacgttttca tcactctgga gtgaatacca cgacgatttc
     1681 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
     1741 ttccctaaag ggtttattga gaatatgttt ttcgtatcag ccaatccctg ggtgagtttc
     1801 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
     1861 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
     1921 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgac
     1981 gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt tgatagatcc
     2041 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt
     2101 ttttattggt gagaatccaa
//
