LOCUS       sequence-471432-        2120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' Operon Connector 7 in the M1 position used to construct operons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_G_0_1-2_007_OC5'7
FEATURES             Location/Qualifiers
     source          1..2120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      608..702
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      885..1473
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1374..1393
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1627..1644
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1668..1725)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1797..1818
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1821..1864
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1868..1886)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(2048..2120,1..587)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(join(2114..2120,1..13))
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
       61 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
      121 gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat
      181 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcac
      241 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
      301 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
      361 tatcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
      421 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
      481 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
      541 ttaatgaatt acaacagtac tgcgacgagt ggcagggcgg ggcgtaattt gatatcgagc
      601 tcgcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      661 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcct cgagctgtca
      721 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
      781 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
      841 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
      901 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
      961 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1021 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1081 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1141 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1201 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     1261 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1321 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1381 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     1441 tgatgctcgt caggggggcg gagcctgtgg aaaaacgcca gcaacgcggc ctttttacgg
     1501 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     1561 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     1621 gagcgcagcg agtcagtgag cgaggaagcc tgcataacgc gaagtaatct tttcggtttt
     1681 aaagaaaaag ggcagggtgg tgacaccttg cccttttttg ccggactgca gcggccgcta
     1741 ctagtcgcga tgagtggtct caagtatacc cgagacgtgt tcgagaccac tcatcgctct
     1801 agaagcggcc gcgaattcca gaaatcatcc ttagcgaaag ctaaggattt tttttatctg
     1861 aaattctgcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     1921 tagacgtcag gtggcactcg agttgatcgg gcacgtaaga ggttccaact ttcaccataa
     1981 tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga
     2041 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
     2101 taaagaacat tttgaggcat
//
