LOCUS       sequence-471434-        2124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Promoter dummy sequence used as a baseline for promoter 
            characterization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_B_0_2_020_Pdummy
FEATURES             Location/Qualifiers
     source          1..2124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      76..170
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      353..941
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     842..861
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1095..1112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1136..1193)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1269..1290
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1293..1336
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1340..1358)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(1520..2124,1..55)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1586..1605)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cagaatgctt aatgaattac aacagtactg cgacgagtgg cagggcgggg cgtaatttga
       61 tatcgagctc gcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg
      121 atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaagcctcg
      181 agctgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
      241 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
      301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      481 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
      541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      901 gatttttgtg atgctcgtca ggggggcgga gcctgtggaa aaacgccagc aacgcggcct
      961 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1021 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     1081 gaacgaccga gcgcagcgag tcagtgagcg aggaagcctg cataacgcga agtaatcttt
     1141 tcggttttaa agaaaaaggg cagggtggtg acaccttgcc cttttttgcc ggactgcagc
     1201 ggccgctact agtcgcgatg agtggtctca agtaaagggg cgccaggggc tcccgagacc
     1261 actcatcgct ctagaagcgg ccgcgaattc cagaaatcat ccttagcgaa agctaaggat
     1321 tttttttatc tgaaattctg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     1381 ataatggttt cttagacgtc aggtggcact cgagttgatc gggcacgtaa gaggttccaa
     1441 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
     1501 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
     1561 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
     1621 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
     1681 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaatttcg
     1741 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
     1801 tttccatgag caaactgaaa cgttttcatc actctggagt gaataccacg acgatttccg
     1861 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
     1921 ccctaaaggg tttattgaga atatgttttt cgtatcagcc aatccctggg tgagtttcac
     1981 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     2041 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     2101 cgtttgtgat ggcttccatg tcgg
//
