LOCUS       sequence-471435-        2326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rrn16 promoter long Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_2_001_rrn16_prom_Cr
FEATURES             Location/Qualifiers
     source          1..2326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(136..230)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(251..910)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     825..844
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1072..1090
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1094..1137)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1140..1161
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1439..1496
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1520..1537)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1691..2279)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1771..1790)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
       61 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
      121 cagctcgagg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
      181 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
      241 cgatatcaaa ttacgccccg ccctgccact cgtcgcagta ctgttgtaat tcattaagca
      301 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
      361 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
      421 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgatacga
      481 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
      541 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagtg
      601 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
      661 tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg
      721 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
      781 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
      841 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
      901 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
      961 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga
     1021 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     1081 atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg
     1141 aattcgcggc cgcttctaga gcgatgagtg gtctcgggag caggcaacaa atttatttat
     1201 tgtcccgtaa ggggaagggg aaaacaatta ttattttact gcggagcagc ttgttattag
     1261 aaatttttat taaaaaaaaa ataaaaattt gacaaaaaaa aataaaaaag ttaaattaaa
     1321 aacactggga atgttctaac aatcataaaa aaatcaaaag ggtttaaaat cccgacaaaa
     1381 tttaaacttt aaagagttac ttgagaccac tcatcgcgac tagtagcggc cgctgcagtc
     1441 cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac
     1501 ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1561 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1621 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1681 tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1741 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1801 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1861 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1921 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1981 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2041 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2101 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     2161 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2221 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2281 aagatccttt gatcttttct acggggtctg acgctcagtg gaacga
//
