LOCUS       sequence-471439-        2534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  atpA 5'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3_002_atpA_5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(227..321)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(342..1001)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     916..935
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1163..1181
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1185..1228)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1231..1252
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1738..1795
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1819..1836)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(1990..2534,1..44))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2070..2089)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
       61 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
      121 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
      181 tctaaagtat atatgagtaa acttggtctg acagctcgag gcttggattc tcaccaataa
      241 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
      301 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
      361 tcgtcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
      421 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
      481 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
      541 gtgaaactca cccagggatt ggctgatacg aaaaacatat tctcaataaa ccctttaggg
      601 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
      661 cggaaatcgt cgtggtattc actccagagt gatgaaaacg tttcagtttg ctcatggaaa
      721 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
      781 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
      841 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
      901 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
      961 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     1021 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     1081 ttggaacctc ttacgtgccc gatcaactcg agtgccacct gacgtctaag aaaccattat
     1141 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
     1201 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agcgatgagt
     1261 ggtctcgtac tactatataa atacatttac ctttttataa atacatttac ctttttttta
     1321 atttgcatga ttttaatgct tatgctatct tttttattta gtccataaaa cctttaaagg
     1381 accttttctt atgggatatt tatattttcc taacaaagca atcggcgtca taaactttag
     1441 ttgcttacga cgcctgtgga cgtccccccc ttccccttac gggcaagtaa acttagggat
     1501 tttaatgcaa taaataaatt tgtcctcttc gggcaaatga attttagtat ttaaatatga
     1561 caagggtgaa ccattacttt tgttaacaag tgatcttacc actcactatt tttgttgaat
     1621 tttaaactta tttaaaattc tcgagaaaga ttttaaaaat aaactttttt aatcttttat
     1681 ttattttttc ttttttaatg tgagaccact catcgcgact agtagcggcc gctgcagtcc
     1741 ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact
     1801 tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1861 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     1921 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1981 gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2041 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2101 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2161 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2221 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2281 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2341 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2401 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2461 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2521 tagcggtggt tttt
//
