LOCUS       sequence-471440-        2203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  petB 5'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3_005_petB_5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      221..809
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     710..729
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     963..980
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1004..1061)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1216..1237
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1240..1283
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1287..1305)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1467..2126
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1533..1552)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      join(2147..2203,1..38)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aagcctcgag ctgtcagacc
       61 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
      121 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
      181 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
      241 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
      301 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
      361 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
      421 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
      481 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
      541 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
      601 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      661 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      721 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      781 gctcgtcagg ggggcggagc ctgtggaaaa acgccagcaa cgcggccttt ttacggttcc
      841 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      901 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
      961 gcagcgagtc agtgagcgag gaagcctgca taacgcgaag taatcttttc ggttttaaag
     1021 aaaaagggca gggtggtgac accttgccct tttttgccgg actgcagcgg ccgctactag
     1081 tcgcgatgag tggtctcaca ttataaaatt tttataagat tgtgacatga ccattaggct
     1141 ttcttaagac taaaaaaatg tgtagcttaa ttatttaaag tttaaattaa agttatagta
     1201 cgagaccact catcgctcta gaagcggccg cgaattccag aaatcatcct tagcgaaagc
     1261 taaggatttt ttttatctga aattctgcct cgtgatacgc ctatttttat aggttaatgt
     1321 catgataata atggtttctt agacgtcagg tggcactcga gttgatcggg cacgtaagag
     1381 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga
     1441 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
     1501 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
     1561 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
     1621 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
     1681 aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
     1741 acaccgtttt ccatgagcaa actgaaacgt tttcatcact ctggagtgaa taccacgacg
     1801 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
     1861 cctatttccc taaagggttt attgagaata tgtttttcgt atcagccaat ccctgggtga
     1921 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     1981 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
     2041 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
     2101 gcgacgagtg gcagggcggg gcgtaatttg atatcgagct cgcttggact cctgttgata
     2161 gatccagtaa tgacctcaga actccatctg gatttgttca gaa
//
