LOCUS       sequence-471443-        2179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  psbE 5'UTR  Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3_008_psbE_5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      213..801
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     702..721
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     955..972
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(996..1053)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1184..1205
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1208..1251
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1255..1273)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1435..2094
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1501..1520)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      join(2115..2179,1..30)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 tgccgccggg cgttttttat tggtgagaat ccaagcctcg agctgtcaga ccaagtttac
       61 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
      121 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
      181 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
      241 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
      301 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
      361 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      421 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      481 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      541 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      601 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
      661 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
      721 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
      781 ggggggcgga gcctgtggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
      841 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
      901 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
      961 tcagtgagcg aggaagcctg cataacgcga agtaatcttt tcggttttaa agaaaaaggg
     1021 cagggtggtg acaccttgcc cttttttgcc ggactgcagc ggccgctact agtcgcgatg
     1081 agtggtctca cattaaaaaa tacctcctaa gtgtgcttac ttagcttttt caacaatttg
     1141 tctgctttat aatcaatgta ttatagtacg agaccactca tcgctctaga agcggccgcg
     1201 aattccagaa atcatcctta gcgaaagcta aggatttttt ttatctgaaa ttctgcctcg
     1261 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     1321 gcactcgagt tgatcgggca cgtaagaggt tccaactttc accataatga aataagatca
     1381 ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag
     1441 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
     1501 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
     1561 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
     1621 cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg
     1681 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
     1741 tcatcactct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
     1801 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
     1861 tttttcgtat cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
     1921 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
     1981 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
     2041 agaatgctta atgaattaca acagtactgc gacgagtggc agggcggggc gtaatttgat
     2101 atcgagctcg cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga
     2161 tttgttcaga acgctcggt
//
