LOCUS       sequence-471444-        2389 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  psbK 5'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3_010_psbK_5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(151..245)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(266..925)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     840..859
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1087..1105
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1109..1152)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1155..1176
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1517..1574
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1598..1615)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1769..2357)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1849..1868)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
       61 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
      121 gtaaacttgg tctgacagct cgaggcttgg attctcacca ataaaaaacg cccggcggca
      181 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
      241 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcgtcg cagtactgtt
      301 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
      361 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
      421 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
      481 gattggctga tacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
      541 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
      601 attcactcca gagtgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
      661 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
      721 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
      781 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
      841 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
      901 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
      961 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     1021 gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     1081 tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct
     1141 aaggatgatt tctggaattc gcggccgctt ctagagcgat gagtggtctc gtacttttga
     1201 ttttagaaag aaaaaacgag ctttaaggtg agcttatttt gttgcgtgta aatttttaaa
     1261 atctaaggtg tatagacaaa aatctacatt ttcatatgct aaaaacatac tctttacggg
     1321 tacgcgaatg ttaggtaaat tttcacaact aactctatgg ttgtgggaag aaaaccaaat
     1381 acatagagat atttttaaaa agatatctct cactttaata gattttatta taaatactat
     1441 caacaatttc ttaaactttt taatgaagga tatttaatgt gagaccactc atcgcgacta
     1501 gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt
     1561 taaaaccgaa aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct
     1621 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1681 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1741 accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc
     1801 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1861 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1921 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1981 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2041 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2101 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2161 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2221 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2281 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2341 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctga
//
