LOCUS       sequence-471446-        2297 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  psaC 5'UTR  Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3_018_psaC_5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(105..199)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(220..879)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     794..813
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1041..1059
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1063..1106)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1109..1130
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1379..1436
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1460..1477)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1631..2219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1711..1730)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
       61 taaagtatat atgagtaaac ttggtctgac agctcgaggc ttggattctc accaataaaa
      121 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
      181 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc
      241 gtcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
      301 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
      361 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
      421 gaaactcacc cagggattgg ctgatacgaa aaacatattc tcaataaacc ctttagggaa
      481 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
      541 gaaatcgtcg tggtattcac tccagagtga tgaaaacgtt tcagtttgct catggaaaac
      601 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
      661 aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
      721 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      781 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      841 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      901 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      961 ggaacctctt acgtgcccga tcaactcgag tgccacctga cgtctaagaa accattatta
     1021 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat
     1081 ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag cgatgagtgg
     1141 tctcgtactt ggaccaaaag cagtcattca aatataggta tgaggagaga aatatatgcg
     1201 ccgcaaggcg cactgccact gcttaatata aataccttgt ttaaagtcga ttctcaatct
     1261 tctttttgat atggagatga catatttagc acaacgcaaa aatatagcat ctttaaaaat
     1321 taacttaaaa tttaaataat gtgagaccac tcatcgcgac tagtagcggc cgctgcagtc
     1381 cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac
     1441 ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1501 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1561 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1621 tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1681 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1741 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1801 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1861 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1921 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1981 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2041 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     2101 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2161 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2221 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2281 ggattttggt catgaga
//
