LOCUS       sequence-471447-        2201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rbcL 5'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3_019_rbcL_5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(61..155)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(176..835)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     750..769
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     997..1015
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1019..1062)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1065..1086
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1239..1296
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1320..1337)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1491..2079)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1571..1590)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg
       61 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
      121 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
      181 ccccgccctg ccactcgtcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
      241 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
      301 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
      361 ttaaatcaaa actggtgaaa ctcacccagg gattggctga tacgaaaaac atattctcaa
      421 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
      481 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagtgatgaa aacgtttcag
      541 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
      601 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
      661 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
      721 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
      781 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
      841 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
      901 cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc
      961 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
     1021 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt
     1081 ctagagcgat gagtggtctc gtactaaatg tatttaaaat ttttcaacaa tttttaaatt
     1141 atatttccgg acagattatt ttaggatcgt caaaagaagt tacatttatt tatataaaat
     1201 gtgagaccac tcatcgcgac tagtagcggc cgctgcagtc cggcaaaaaa gggcaaggtg
     1261 tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc
     1321 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1381 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1441 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg
     1501 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1561 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1621 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1681 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1741 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1801 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1861 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1921 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1981 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2041 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2101 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2161 tcaaaaagga tcttcaccta gatcctttta aattaaaaat g
//
